A5027796676- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- GABA and Rice Research
- Synthesis of heterocyclic compounds
- Biosensors and Analytical Detection
- Biochemical and Molecular Research
- Pharmacogenetics and Drug Metabolism
- Rice Cultivation and Yield Improvement
- Fungal Biology and Applications
- Chemical Reaction Mechanisms
- Analytical Chemistry and Chromatography
- Microbial Metabolism and Applications
- Advanced Nanomaterials in Catalysis
- Synthesis and Biological Evaluation
- Chemical Synthesis and Reactions
- Asymmetric Hydrogenation and Catalysis
- Electrochemical sensors and biosensors
- Enzyme Production and Characterization
- Synthesis and Reactions of Organic Compounds
- SARS-CoV-2 detection and testing
Shanghai Jiao Tong University
2025
Taiyuan University of Technology
2024
Wuhan University
2014-2023
University of Jinan
2006-2023
Wuhan Donghu University
2016-2021
Shanghai Institute of Organic Chemistry
2017
Chinese Academy of Sciences
2013-2017
East China University of Science and Technology
2010-2014
Zhejiang Hisun Pharmaceutical (China)
2014
Fujian Academy of Agricultural Sciences
2003-2011
A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For first time, (R)-HPBE >99% ee produced via bioreduction of OPBE at 1 M without external addition cofactors. The space-time yield (700 g·L–1·d–1) 27 times higher than highest record.
Abstract In order to search for oxidoreductases suitable the preparation of methyl ( R )‐ o ‐chloromandelate [( )‐CMM], key intermediate clopidogrel, homologous proteins Gre2p were expressed in Escherichia coli , among which CgKR1 showed most satisfactory activity and stereoselectivity towards ‐chlorobenzoylformate (CBFM). Using crude enzyme glucose dehydrogenase (GDH), as much 300 g⋅L −1 CBFM was almost stoichiometrically converted )‐CMM with excellent enantiomeric excess (98.7% ee ). More...
Tetrahydroisoquinolines (THIQs) with a C1-aryl-substituted groups are common in many natural and synthetic compounds of biological importance. Currently, their enantioselective synthesis primarily reliant on chemical catalysis. Enzymatic using imine reductase is very attractive, because the cost-effectiveness, high catalytic efficiency, enantioselectivity. However, steric hindrance 1-aryl substituents make this conversion challenging, current successful examples mostly restricted to simple...
Natural products are critical for drug discovery and development; however their is challenged by the wide inactivation or silence of microbial biosynthetic pathways. Currently strategies targeting this problem mainly concentrated on chromosome dissembling, transcription, translation-stage regulations as well chemical stimulation. In study, we developed a novel approach to awake cryptic/silenced pathways through augmentation conserved protein modification step-phosphopantetheinylation carrier...
Selective modification of carbon scaffolds via biosynthetic engineering is important for polyketide structural diversification. Yet, this scope currently restricted to simple aliphatic groups due (1) limited variety CoA-linked extender units, which lack aromatic structures and chemical reactivity, (2) narrow acyltransferase (AT) specificity, units. In report, we uncovered characterized the first unit benzylmalonyl-CoA from pathways splenocin enterocin in Streptomyces sp. CNQ431. Its...
Abstract Background Monascus azaphilone pigments (MonAzPs), which were produced by species, have been used as important food colorant and supplements for more than one billion people during their daily life. Moreover, MonAzPs recently received attention because of diverse physiological activities. However, the high microbial production is still not always guaranteed. Herein, aim this study was to develop an efficient biotechnological process production. Results In study, exogenous cyclic...
Significance Enzymatic modifications of anthracycline antibiotics are urgently needed in the fields biosynthesis, biocatalysis, and even medical chemistry. However, neither hydroxyl regioisomerization nor dehydroxylation core was described previously. Here, we discover an unprecedented process biosynthesis a rare carbon-4 deoxyanthracycline, which includes three tailoring steps performed by four-enzyme cascade: two-component hydroxylases mediated cryptic hydroxylation, two NmrA-like...
Abstract A versatile keto ester reductase Cg KR1, exhibiting a broad substrate spectrum, was obtained from Candida glabrata by genome data mining. It showed the highest activity toward an aliphatic β‐keto ester, ethyl 4‐chloro‐3‐oxobutanoate (COBE), but much lower bulkier α‐keto esters with aromatic group, such as methyl ortho ‐chlorobenzoylformate (CBFM) and 2‐oxo‐4‐phenylbutyrate (OPBE). By rational design of active pocket, specificity significantly altered this tailor‐made higher (∼7‐fold...
Angustmycin A has anti-mycobacterial and cytokinin activities, contains an intriguing structure in which unusual sugar with C5'-C6' dehydration is linked to adenine via N-glycosidic bond. However, the logic underlying biosynthesis of this molecule long remained obscure. Here, we address angustmycin by full deciphering its pathway. We demonstrate that AgmD, C, A, E, B function as D-allulose 6-phosphate 3-epimerase, pyrophosphokinase, phosphoallulosyltransferase, phosphoribohydrolase,...
Thienodolin (THN) features a tricyclic indole-S-hetero scaffold that encompasses two unique carbon-sulfur bonds. Although its biosynthetic gene cluster has been recently identified in Streptomyces albogriseolus, the essential enzymes for formation of C-S bonds have relatively unexplored. Here, we isolated and characterized new from sp. FXJ1.172. Heterologous expression, systematic inactivation, vitro biochemical characterization enable us to determine minimum set genes THN synthesis, an...
Halohydrin dehalogenases (HHDHs) are biocatalytically interesting enzymes due to their ability form C-C, C-N, C-O, and C-S bonds. One of most important application HHDH was the protein engineering HheC (halohydrin dehalogenase from Agrobacterium radiobacter AD1) for industrial manufacturing ethyl (R)-4-cyano-3-hydroxybutanoate (HN), a key chiral synthon cholesterol-lowering drug atorvastatin. During our development an alternative, more efficient economic route chemo-enzymatic preparation...
T-2 toxin, one of the type A trichothecenes, presents a potential hazard to human and animal health. Our previous work demonstrated that porcine cytochrome P450 3A29 (CYP3A29) played an important role in hydroxylation toxin. To identify amino acids involved this metabolic process, toxin was docked into homology model CYP3A29 based on crystal structure CYP3A4 using AutoDock 4.0. Nine residues CYP3A29, Arg105, Arg106, Phe108, Ser119, Lys212, Phe213, Phe215, Arg372 Glu374, which were found...