A5081321173- Microbial Natural Products and Biosynthesis
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Regulation of Appetite and Obesity
- Mesenchymal stem cell research
- Chronic Myeloid Leukemia Treatments
- Cancer-related molecular mechanisms research
- Retinoids in leukemia and cellular processes
- Multiple Myeloma Research and Treatments
- Biochemical Analysis and Sensing Techniques
- X-ray Diffraction in Crystallography
- Enzyme Structure and Function
- HIV/AIDS drug development and treatment
- Chronic Lymphocytic Leukemia Research
- Crystallization and Solubility Studies
- Bone and Joint Diseases
- Adipose Tissue and Metabolism
- Magnesium in Health and Disease
- Polyamine Metabolism and Applications
- Cancer Mechanisms and Therapy
- Amoebic Infections and Treatments
- Neutropenia and Cancer Infections
- Diet and metabolism studies
Howard Hughes Medical Institute
2015-2025
Janelia Research Campus
2020-2025
First Affiliated Hospital of Kunming Medical University
2015-2024
Kunming Medical University
2015-2024
Zhongnan Hospital of Wuhan University
2022-2024
Wuhan University
2016-2024
Chinese Institute for Brain Research
2024
Beijing Normal University
2024
Shanxi Medical University
2005-2023
Shanxi Academy of Medical Sciences
2017-2023
A receptor for gut hormones also functions in the brain, where its loss affects attention.
The present study aimed to investigate the effects of sodium butyrate on intestinal barrier and mast cell activation, as well inflammatory mediator production, determine whether mitogen-activated protein kinase signaling pathways are involved in these processes. A total 72 piglets, weaned at 28 ± 1 d age, were allotted two dietary treatments (control vs. 450 mg/kg butyrate) for 2 wk. results showed that supplemental increased daily gain, improved morphology, indicated by greater villus...
Abstract Glycine decarboxylase (GLDC) is a key enzyme of glycine cleavage system that converts into one-carbon units. GLDC commonly up-regulated and plays important roles in many human cancers. Whether how regulated by post-translational modifications unknown. Here we report mechanistic target rapamycin complex 1 (mTORC1) signal inhibits acetylation at lysine (K) 514 inducing transcription the deacetylase sirtuin 3 (SIRT3). Upon inhibition mTORC1, acetyltransferase acetyl-CoA (ACAT1)...
Hedonic eating is defined as food consumption driven by palatability without physiological need. However, neural control of palatable intake poorly understood. We discovered that hedonic controlled a pathway from the peri–locus ceruleus to ventral tegmental area (VTA). Using photometry-calibrated optogenetics, we found VTA dopamine (VTA DA ) neurons encode bidirectionally regulate consumption. neuron responsiveness was suppressed during semaglutide, glucagon-like peptide receptor 1 (GLP-1R)...
Aurantimycin (ATM), produced by Streptomyces aurantiacus JA 4570, is a potent antimicrobial and antitumor antibiotic. Although the chemical structure of ATM highly distinctive features cyclohexadepsipeptide scaffold attached with C14 acyl side chain, little known about its biosynthetic pathway regulatory mechanism.In this work, we report identification characterization gene cluster from S. 4570. Targeted inactivation artG, coding for NRPS enzyme, completely abolished production, thereof...
Background Aberrant DNA methylation occurs frequently in cancer. The aim of this study was to identify novel markers lung cancer Xuanwei, China, through integrated genome-wide and gene expression studies. Methods Differentially methylated regions (DMRs) differentially expressed genes (DEGs) were detected on 10 paired tissues noncancerous by immunoprecipitation combined with microarray (MeDIP-chip) analyses, respectively. Integrated analysis DMRs DEGs performed screen out candidate...
Tubercidin (TBN), an adenosine analog with potent antimycobacteria and antitumor bioactivities, highlights intriguing structure, in which a 7-deazapurine core is linked to the ribose moiety by N-glycosidic bond. However, molecular logic underlying biosynthesis of this antibiotic has remained poorly understood. Here, we report discovery characterization TBN biosynthetic pathway from Streptomyces tubercidicus NBRC 13090 via reconstitution its production heterologous host. We demonstrated that...
FOR-A and PRF-A are C -nucleoside antibiotics known for their unusual chemical structures remarkable biological activities. Deciphering the enzymatic mechanism construction of a scaffold during FOR-A/PRF-A biosynthesis will not only expand biochemical repertoire novel reactions but also permit target-oriented genome mining FOR-A/PRF-A-related antibiotics. Moreover, availability biosynthetic gene clusters pave way rational generation designer derivatives with enhanced/selective bioactivity...
The morbidity and mortality of lung cancer in Xuanwei, China, are the highest world. This study attempts to identify differentially expressed genes (DEGs) related adenocarcinoma Xuanwei. expression profiles eight paired tissues corresponding nontumor were acquired by microarrays. Functional annotations DEGs carried out bioinformatics analysis. results microarrays further verified real-time quantitative PCR (RTq-PCR). A total 5290 classified as Xuanwei; 3325 upregulated 1965 downregulated,...
Non-heme Fe2+ and 2-oxoglutarate (Fe2+/2OG)-dependent halogenases are a promising platform for biocatalytic halogenations owing to their ability functionalize unactivated sp3 C–H bonds. To date, however, relatively few Fe2+/2OG-dependent have been identified that act on small stand-alone molecules. AdaV, member of the carrier-protein-independent halogenases, selectively modifies C2′ dAMP in 2′-chloropentostatin biosynthesis. In this study, we report X-ray crystallographic structures AdaV...
This study was aimed at investigating whether dietary copper/zinc-loaded montmorillonite (Cu/Zn-Mt) could alleviate Escherichia coli LPS-induced intestinal injury through pro- and anti-inflammatory signaling pathways (TLRs, NLRs TGF-β1) in weaned piglets. Eighteen 21-d-old pigs were randomly divided into three groups (control, LPS + Cu/Zn-Mt). After 21 d of feeding, the group Cu/Zn-Mt received i.p. administration LPS, whereas control saline. At 4 h post-injection, jejunum samples collected...
Purine nucleoside antibiotic pairs, concomitantly produced by a single strain, are an important group of microbial natural products. Here, we report target-directed genome mining approach to elucidate the biosynthesis purine pair aristeromycin (ARM) and coformycin (COF) in Micromonospora haikouensis DSM 45626 (a new producer for ARM COF) Streptomyces citricolor NBRC 13005 COF producer). We also provide biochemical data that MacI MacT function as unusual phosphorylases, catalyzing...
ABSTRACT Polyoxin (POL) is an unusual peptidyl nucleoside antibiotic, in which the moiety and skeleton are linked by amide bond. However, their biosynthesis remains poorly understood. Here, we report deciphering of PolG as ATP-dependent ligase responsible for assembly POL. A polG mutant capable accumulating multiple intermediates, including (carbamoylpolyoxamic acid [CPOAA]) skeletons (POL-C previously overlooked thymine POL-C). We further demonstrate that employs mechanism bond formation...
To investigate the efficacy and toxicity of modified FLAG CAG on relapsed or refractory acute myeloid leukemia (AML).Sixty-one patients with AML were divided into group. In group: G-CSF 200 μg·m⁻²·d⁻¹ days 0-5; fludarabine 30 mg·m⁻²·d⁻¹ 1-5; Ara-C 1.0 g·m⁻²·d⁻¹ 1-5. 10 mg·m⁻²·12 h⁻¹ 1-14, aclarubicin 20 mg/d 1-4, 0 1-14.The complete response (CR) rate was 43% (12/28) partial (PR) 18% (5/28) overall (OR) 61% in CR 21% (7/33) PR 15% (5/33) OR 36% There significant statistical difference...
Lung cancer is one of the most frequently diagnosed cancers worldwide, associated with a poor survival rate. Taxol (Paclitaxel) commonly used as chemotherapeutic treatment for advanced lung cancers. While has improved clinical outcomes patients, significant number them develop resistance to Taxol, resulting in failure. The role long noncoding RNA HCG18 and not yet been fully understood. To investigate this, we examined expression miR-34a-5p tumors normal tissues using qRT-PCR. We also...
ABSTRACT Formycin A (FOR-A) and pyrazofurin (PRF-A) are purine-related C -nucleoside antibiotics, in which ribose a pyrazole-derived base linked by -glycosidic bond, however, the logic underlying biosynthesis of these molecules has remained largely unexplored. Here, we report discovery pathways for FOR-A PRF-A from diverse actinobacteria, demonstrate that their is initiated lysine N 6 -monooxygenase. Moreover, show forT prfE (individually related to biosynthesis) mutants correspondingly...