A5029257186- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Silicon Effects in Agriculture
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Silk-based biomaterials and applications
- Insect and Arachnid Ecology and Behavior
- Biosensors and Analytical Detection
- Light effects on plants
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Monoclonal and Polyclonal Antibodies Research
- Aluminum toxicity and tolerance in plants and animals
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Plant nutrient uptake and metabolism
- Tree Root and Stability Studies
- Polysaccharides and Plant Cell Walls
- Glycosylation and Glycoproteins Research
Zhejiang A & F University
2016-2025
Ministry of Agriculture and Rural Affairs
2022-2025
First Affiliated Hospital of Kunming Medical University
2016-2024
Kunming Medical University
2008-2024
State Key Laboratory of Silkworm Genomic Biology
2011-2015
Southwest University
2011-2015
Second Affiliated Hospital of Kunming Medical College
2008
Donghua University
2008
Fudan University
2002-2005
State Key Laboratory of Genetic Engineering
2002-2004
Human utilization of the mulberry–silkworm interaction started at least 5,000 years ago and greatly influenced world history through Silk Road. Complementing silkworm genome sequence, here we describe a mulberry species Morus notabilis. In 330-Mb assembly, identify 128 Mb repetitive sequences 29,338 genes, 60.8% which are supported by transcriptome sequencing. Mulberry gene appear to evolve ~3 times faster than other Rosales, perhaps facilitating species' spread worldwide. The tree is among...
Temporally regulated microRNAs have been identified as master regulators of developmental timing in both animals and plants. In plants, vegetative development is by a temporal decrease miR156 level, but how this decreased expression initiated then maintained during shoot remains elusive. Here, we show that miR159 required for the correct Arabidopsis thaliana Loss increases level throughout delays development, whereas overexpression slightly accelerated development. The repression...
After germination, plants progress through juvenile and adult phases of vegetative development before entering the reproductive phase. The character timing these vary significantly between different plant species, which makes it difficult to know whether temporal variations in various traits represent same, or different, developmental processes. miR156 has been shown be master regulator plants. Overexpression prolongs phase development, whereas knocking-down level promotes development....
Silicon (Si) is a widely recognized beneficial element in plants. With the emergence of nanotechnology agriculture, silicon nanoparticles (SiNPs) demonstrate promising applicability sustainable agriculture. Particularly, application SiNPs has proven to be high-efficiency and cost-effective strategy for protecting plant against various biotic abiotic stresses such as insect pests, pathogen diseases, metal stress, drought salt stress. To date, rapid progress been made unveiling multiple...
Plants progress from a juvenile vegetative phase of development to an adult before they enter the reproductive phase. miR156 has been shown be master regulator juvenile-to-adult transition in plants. However, mechanism how is transcriptionally regulated still remains elusive. In forward genetic screen, we identified that mutation SWI2/SNF2 chromatin remodeling ATPase BRAHMA (BRM) exhibited accelerated change phenotype by reducing expression miR156, which turn caused corresponding increase...
Cadmium (Cd) pollution is one of the major threats in agricultural production, and can cause oxidative damage growth limitation plants. MicroRNA398 (miR398) involved plant resistance to different stresses, post-transcriptional regulation miR398 on CSDs plays a key role. Here, we report that was down-regulated tomato response Cd stress. Simultaneously, CSD1 SOD were up-regulated, with CSD2 unchanged, suggesting miR398-induced under In addition, role tolerance evaluated using transgenic line...
Heat stress is one of the most important environmental problems in agriculture, which severely restricts growth and yield plants. In plants, microRNA398 (miR398) negatively regulates activity superoxide dismutase (SOD) by modulating expression its coding genes (CSDs) post-transcriptionally, thereby regulating reactive oxygen species (ROS) homeostasis resistance. this study, role miR398 heat tolerance tomatoes was investigated. Under stress, upregulated tomatoes, while target (CSD1 CSD2) SOD...
Abstract Protein digestion inside the nanoreactor channels of mesoporous silica (SBA‐15) is reported, and evaluated by using peptide‐mass mapping. Both proteases substrates were efficiently captured within these biocompatible nanoreactors. After 10 minutes, mass spectrum protein digests released from mesoporous‐silica‐based nanoreactors revealed presence eight peptides covering 58 % sequence with an intense signal (signal/noise ratio >70). In comparison, conventional overnight in‐solution...
Higher plants progress through a juvenile and an adult phase of development before they enter the reproductive phase. The transition from to is referred as vegetative change, this signified by production trichomes on abaxial side leaf blades in Arabidopsis; however, molecular mechanism underlying process poorly understood. We identified dominant mutation (gl1-D) forward genetic screen that accelerates trichome during shoot development. This phenotype result G-to-A substitution 3' noncoding...
The design and characterization of titania-based alumina-based Poly(dimethylsiloxane) (PDMS) microfluidics enzymatic-reactors along with their analytical features in coupling MALDI-TOF ESI−MS were reported. Microfluidics microchannel stainless steel tubing (SST) fabricated using PDMS casting O2-plasma techniques, used for the preparation an enzymatic-reactor. Plasma oxidation microfluidic system enabled channel wall to present a layer silanol (SiOH) groups. These SiOH groups act as anchors...
MicroRNAs (miRNAs) have been shown to be critical components in plant immunity. MicroRNA398 (miR398) is a highly conserved miRNA all land plants and plays crucial roles diverse biotic stress responses. However, the role of miR398 has not yet characterized tomato resistance against Botrytis cinerea. In this report, transcript levels sly-miR398b were strongly decreased B. cinerea-infected leaves overexpression resulted enhanced susceptibility. The attenuated expression cytosol Cu/Zn-SOD...
The midgut is the major organ for food digestion, nutrient absorption and also a barrier foreign substance. 5th-instar larval stage of silkworm very important growth, development, silk production. In present study, we used 2-DE matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze proteins from larvae as well under starvation condition. A total 96 were identified in this study; among them, 69 observed first time. We found that responded by...
The design and characterization of two kinds poly(dimethylsiloxane) (PDMS) microfluidic enzymatic-reactors along with their analytical utility coupled to MALDI TOF ESI MS were reported. Microfluidic devices integrated microchannel stainless steel tubing (SST) was fabricated using a PDMS casting technique, used for the preparation enzymatic-reactor. chemical modification performed by introducing carboxyl groups surface based on ultraviolet graft polymerization acrylic acid. covalent physical...
Abstract The nodulin 26-like intrinsic protein (NIP) family belonging to a group of aquaporin proteins is unique plants. NIPs have wide transport activities and are involved in developmental processes stress tolerance. well reported Lsi1 Lsi6 NIP III were characterized as Si transporters. However, except Lsi6, most remain unknown. Here, we identified 43 putative aquaporins tomato. We found there 12 NIPs, including 8 I proteins, 3 II 1 among the aquaporins. Also, two efflux transporters...
Multidimensional LC-tandem MS was used to investigate the protein compositions of three tissues silkworm, Bombyx mori. A total 162, 259, and 175 peptides from silkworm larval integument trachea, adult scale obtained by database search were matched 48, 51, 40 proteins, respectively. Forty-one cuticular proteins identified covered all five families silkworm. In scale, seven for first time in final pellet after SDS extraction. The majority found each tissue differentially, suggesting that...
Summary Tendril is a morphological innovation during plant evolution, which provides the plants to obtain climbing ability. However, tendril morphogenesis poorly understood. A novel defective mutant ( tmd1 ) was identified in cucumber. The apical part of replaced by leaf blade mutant, and it lost Map‐based cloning, qPCR detection, bioinformatic analysis, yeast one‐hybrid assay, electrophoretic mobility shift luciferase assay were used explore molecular mechanism CsaTMD1 regulating...
Abstract As a climbing organ, the tendril undergoes rapid elongation to increase its length locate support within short growth time. However, molecular mechanism underlying this observation is poorly understood. Here, development was divided into 4 stages in cucumber (Cucumis sativus L.) along with growth. Phenotypic observations and section analyses showed that of primarily happened during stage 3 mainly due cell expansion. RNA-seq analysis PACLOBUTRAZOL-RESISTANCE4 (CsPRE4) highly...
The tendril is a climbing organ in cucurbits and functions physical support to avoid shading by neighboring vegetation. However, how produce tendrils obtain ability largely unknown. In this study, phenotypes were investigated during different developmental stages. Our results revealed that growth exhibited an age-dependent pattern cucurbits. Tendril was inhibited, the formed as short [nonfunctional (nonF-tendril), approximately 0.1 cm] seedling stage. contrast, enhanced cell proliferation...