A5072517724- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Metal-Catalyzed Oxygenation Mechanisms
- Enzyme-mediated dye degradation
- Oxidative Organic Chemistry Reactions
- Pharmacogenetics and Drug Metabolism
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Catalysis for Biomass Conversion
- bioluminescence and chemiluminescence research
- Biochemical Acid Research Studies
- Carbohydrate Chemistry and Synthesis
- Asymmetric Hydrogenation and Catalysis
- Monoclonal and Polyclonal Antibodies Research
- Advanced Nanomaterials in Catalysis
- Aquatic Ecosystems and Phytoplankton Dynamics
- Biofuel production and bioconversion
- Covalent Organic Framework Applications
- Mesoporous Materials and Catalysis
- Electrocatalysts for Energy Conversion
- Water Quality Monitoring and Analysis
- Aldose Reductase and Taurine
Eindhoven University of Technology
2018-2024
Xinjiang Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Guangxi University
2023
Guizhou Normal University
2022
University of Groningen
2020
Nanjing Tech University
2016-2019
State Key Laboratory of Materials-Oriented Chemical Engineering
2019
Institute of Forest Ecology, Environment and Protection
2018
Chinese Academy of Forestry
2017-2018
It's not easy being green: Biocatalysis is generally recognized as an environmentally benign technology. This green claim, however, seldom substantiated. The use of simple mass-based metrics such the E factor may represent appropriate compromise between significance and effort.
Abstract Peroxygenases catalyze a broad range of (stereo)selective oxyfunctionalization reactions. However, to access their full catalytic potential, peroxygenases need balanced provision hydrogen peroxide achieve high activity while minimizing oxidative inactivation. Herein, we report an enzymatic cascade process that employs methanol as sacrificial electron donor for the reductive activation molecular oxygen. Full oxidation is achieved, generating three equivalents can be used completely...
Peroxygenases are promising oxyfunctionalisation catalysts for organic synthesis.
Metal–organic polyhedra (MOPs) have attracted great attention due to their intriguing structure. However, the applications of MOPs are severely hindered by two shortcomings, namely low dispersity and poor stability. Here we report introduction four (constructed from dicopper carboxylates) cavity-structured mesoporous silica SBA-16 via a double-solvent strategy overcome both shortcomings simultaneously. By judicious design, dimension is just between size cavities entrances SBA-16, MOP...
Abstract Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with ratiometric bioluminescent readout. The concept entails analyte-induced...
Light-driven oxidation of water catalyzed by a photocatalyst can fuel the activity LPMOs, key enzymes in processing recalcitrant polysaccharides.
Peroxygenases are very promising catalysts for oxyfunctionalization reactions. Their practical applicability, however, is hampered by their sensitivity against the oxidant (H2O2), therefore necessitating in situ generation of H2O2. Here, we report a photoelectrochemical approach to provide peroxygenases with suitable amounts H2O2 while reducing electrochemical overpotential needed reduction molecular oxygen When tethered on single-walled carbon nanotubes (SWNTs) under illumination, flavins...
Antibodies are important biomarkers in clinical diagnostics addition to being increasingly used for therapeutic purposes. Although numerous methods their detection and quantification exist, they predominantly require benchtop instruments operated by specialists. To enable the of antibodies at point-of-care (POC), development simple rapid assay independent laboratory equipment is high relevance. In this study, we demonstrate microfluidic thread-based analytical devices (μTADs) as a new...
A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For first time, (R)-HPBE >99% ee produced via bioreduction of OPBE at 1 M without external addition cofactors. The space-time yield (700 g·L–1·d–1) 27 times higher than highest record.
The hyperthermophile Pyrococcus furiosus catalyses the hydrogenation of a broad range carboxylic acids selectively to corresponding primary alcohols. Other functional groups such as isolated C=C-double bonds are not touched. chemoselectivity carboxylate reduction may be directed towards aldehydes by simple medium engineering.
Abstract In order to search for oxidoreductases suitable the preparation of methyl ( R )‐ o ‐chloromandelate [( )‐CMM], key intermediate clopidogrel, homologous proteins Gre2p were expressed in Escherichia coli , among which CgKR1 showed most satisfactory activity and stereoselectivity towards ‐chlorobenzoylformate (CBFM). Using crude enzyme glucose dehydrogenase (GDH), as much 300 g⋅L −1 CBFM was almost stoichiometrically converted )‐CMM with excellent enantiomeric excess (98.7% ee ). More...
Abstract The vanadium‐dependent chloroperoxidase from Curvularia inaequalis is an efficient biocatalyst for the in situ generation of hypohalous acids and subsequent electrophilic oxidation/halogenation reactions. Especially, its superb activity stability under operational conditions make it attractive catalyst organic synthesis. Herein, bromination thymol was investigated, turnover numbers enzyme were found to exceed 2 000 000. major novelty work that vanadium more useful as a brominating...
Bioluminescent sensor proteins provide attractive tools for applications ranging from in vivo imaging to point-of-care testing. Here we introduce a new class of ratiometric bioluminescent that do not rely on direct modulation BRET efficiency, but are based competitive intramolecular complementation split NanoLuc luciferase. Proof concept the feasibility this principle was provided by developing blue-red light emitting protein detection anti-HIV1-p17 antibodies with 500% change emission ratio...
Selective adsorption and efficient regeneration are two crucial issues for processes; unfortunately, only one of them instead both is favored by traditional adsorbents with fixed pore orifices. Herein, we fabricated a new generation smart through grafting photoresponsive molecules, namely, 4-(3-triethoxysilylpropyl-ureido)azobenzene (AB-TPI), onto orifices the support mesoporous silica. The azobenzene (AB) derivatives serve as molecular gates mesopores reversibly opened closed upon light...
Monitoring the levels of therapeutic antibodies in individual patients would allow patient-specific dose optimization, with potential for major and financial benefits. Our group recently developed a new platform bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that antibody detection directly blood plasma. In this study, we targeted four clinically important antibodies, Her2-receptor targeting trastuzumab, anti-CD20 rituximab obinutuzumab, EGFR-blocking cetuximab. A...
Abstract A β‐ketoacyl‐ACP reductase (FabG) gene from Bacillus sp. ECU0013 was heterologously overexpressed in Escherichia coli and the encoded protein purified to homogeneity. The recombinant could reduce a broad spectrum of prochiral ketones including aromatic keto esters showed highest activity asymmetric reduction ethyl 2‐oxo‐4‐phenylbutyrate (OPBE). Using E. cells coexpressing both FabG glucose dehydrogenase (GDH) genes, as much 620 g⋅L −1 OPBE almost stoichiometrically converted ( S...
The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as medium together with the immobilized biocatalyst and tertBuOOH oxidant. Stability activity issues still have be addressed. Nevertheless, gram-scale production enantiopure achieved respectable 90,000 turnovers biocatalyst.
A carbonyl reductase gene (yueD) from Bacillus sp. ECU0013 was heterologously overexpressed in Escherichia coli, and the encoded protein (BYueD) purified to homogeneity characterized. The NADPH-dependent showed a broad substrate spectrum towards different aromatic ketones, α- β-ketoesters. Although enantioselectivity high moderate for reduction of α-ketoesters, all tested β-ketoesters ketones were reduced corresponding chiral alcohols enantiomerically pure forms. Furthermore, practical...
Optically pure α-hydroxy acids and their derivatives are versatile chiral building blocks in the pharmaceutical industry. In this study, potential of a recombinant Pseudomonas putida esterase (rPPE01) for enzymatic resolution α-acetoxy was significantly improved by combinatorial engineering both biocatalyst substrate. Semirational design based on homologous modeling molecular docking provided single-point variant, W187H, whose kcat/KM sodium 2-acetoxy-2-(2′-chlorophenyl)acetate (Ac-CPA-Na)...
Here we present LUCOS (Luminescent Competition Sensor), a modular and broadly applicable bioluminescent diagnostic platform enabling the detection of both small molecules protein biomarkers. The construction sensors entails covalent site-specific coupling sensor component to an analyte-specific antibody via G-mediated photoconjugation. Target is accomplished through intramolecular competition with tethered analyte competitor for binding. We established two variants LUCOS: inherent...