A5008997636- Antiplatelet Therapy and Cardiovascular Diseases
- Platelet Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Hemostasis and retained surgical items
- Bone Tissue Engineering Materials
- Surgical Sutures and Adhesives
- Inflammatory mediators and NSAID effects
- Blood groups and transfusion
- Atrial Fibrillation Management and Outcomes
- Traditional Chinese Medicine Analysis
- Coenzyme Q10 studies and effects
- Nuclear Receptors and Signaling
- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood Coagulation and Thrombosis Mechanisms
- Antioxidant Activity and Oxidative Stress
- NF-κB Signaling Pathways
- Coronary Interventions and Diagnostics
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Periodontal Regeneration and Treatments
- Myasthenia Gravis and Thymoma
McMaster University
2024-2025
University of Toronto
2016-2024
St. Michael's Hospital
2016-2024
Unity Health Toronto
2019-2023
University of Washington
2023
Bloodworks Northwest
2023
Universidad de Salamanca
2023
Toronto Public Health
2019
Centre of Biomedical Research
2019
Abstract Platelet αIIbβ3 integrin and its ligands are essential for thrombosis hemostasis, play key roles in myocardial infarction stroke. Here we show that apolipoprotein A-IV (apoA-IV) can be isolated from human blood plasma using platelet β3 integrin-coated beads. Binding of apoA-IV to platelets requires activation integrin, the direct apoA-IV-αIIbβ3 interaction detected a single-molecule Biomembrane Force Probe. We identify aspartic acids 5 13 at N-terminus required binding which is...
Summary Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by antiplatelet autoantibodies, with many patients refractory or relapsing on conventional treatments. GPIbα, important autoantigen in ITP, notably linked to refractoriness, highlighting the need for novel We assessed CD19 chimeric antigen receptor (CAR)‐T cell therapy's potential a modified murine model targeting GPIbα. CAR‐T infusion accelerated platelet count recovery compared control group, effectively depleted...
Salvianolic acid B (SAB) is a major component of
Platelet integrin αIIbβ3 is the key mediator of atherothrombosis. Supplementation coenzyme Q10 (CoQ10), a fat-soluble molecule that exists in various foods, exerts protective cardiovascular effects. This study aims to investigate whether and how CoQ10 acts on signaling thrombosis, major cause diseases.Using series platelet functional assays vitro, it demonstrated reduces human aggregation, granule secretion, spreading, clot retraction. It further inhibits outside-in signaling. These...
This study evaluates the hemostatic properties of tantalum-containing mesoporous bioactive glasses (Ta-MBGs) through a suite in-vitro methods: hemolysis percentage, zeta potential, blood coagulation assays (Activated Partial Thromboplastin Time – APTT and Prothrombin - PT) cytotoxicity tests. Five compositions Ta-MBG, with x mol% Ta 2 O 5 added to glass series (80- x)SiO -15CaO-5P xTa where x=0 (0Ta), x=0.5 (0.5Ta), x=1 (1Ta), x=5 (5Ta), x=10 (10Ta) mol%, were synthesised. The potential all...
Synthesis and characterization of the first mesoporous bioactive glasses (MBGs) containing tantalum are reported here, along with their potential application as hemostats. Silica MBGs were synthesized using molar composition (80-x)% Si, 15% Ca, 5% P, x% Ta. It was found that incorporation >1 mol % Ta into changes physical chemical properties. Increasing content from 0 to 10 causes a decrease in surface area pore volume ~20 ~35%, respectively. This is due increase nonbridging oxygens mismatch...
Abstract Apoptotic-like phase is an essential step in thrombopoiesis from megakaryocytes. Anthocyanins are natural flavonoid pigments that possess a wide range of biological activities, including protection against cardiovascular diseases and induction tumour cell apoptosis. We investigated the effects underlying mechanisms cyanidin-3-o-β-glucoside (Cy-3-g, major bioactive compound anthocyanins) on apoptosis human primary megakaryocytes Meg-01 line vitro. found Cy-3-g dose-dependently...
Coenzyme Q10 (CoQ10) exists in a wide variety of foods and has promising cardiovascular benefits. However, its effects on platelets integrin αIIbβ3 signaling during atherosclerosis have not been previously explored. Here, apolipoprotein E-deficient (ApoE-/-) mice were fed standard diet, high-fat diet (HFD) or CoQ10-supplemented HFD for 12 weeks. We found that CoQ10 supplementation ApoE-/- significantly alleviated formation HFD-induced atherosclerotic lesions, attenuated platelet...
Acetylsalicylic acid (ASA), also known as aspirin, appears to be ineffective in inhibiting platelet aggregation 20–30% of patients. Light transmission aggregometry (LTA) is a gold standard function assay. In this pilot study, we used LTA personalize ASA therapy ex vivo atherosclerotic Patients were recruited who on 81 mg ASA, presenting ambulatory clinics at St. Michael’s Hospital (n = 64), with evidence disease defined clinical symptoms and diagnostic findings indicative symptomatic...
Background and Objectives: Aspirin (acetylsalicylic acid—ASA) is a first-line antiplatelet therapy provided to patients with coronary artery disease (CAD). However, it has been demonstrated that 20–30% of these are non-sensitive their ASA therapy. non-sensitivity phenomenon where low-dose (81–325 mg) does not completely inhibit arachidonic-acid-induced platelet aggregation, putting at risk adverse cardio-thrombotic events. Ticagrelor P2Y12 receptor inhibitor alternative approved reduce the...
We report a case of pure white cell aplasia (PWCA) postthymoma resection in 74-year-old male presenting with 2-week history fevers, night sweats, and severe febrile neutropenia. His was treated intravenous immunoglobulin (IVIg), granulocyte colony-stimulating factor (G-CSF), prednisone, cyclosporine mixed response. He also developed immune thrombocytopenia, which responded well to short course eltrombopag. With continued treatment, his platelet counts were stable after stopping The patient's...
Haemorrhage is the leading cause of battlefield deaths and second most common for civilian mortality worldwide. Biomaterials-based haemostatic agents are used to aid in bleeding stoppage; mesoporous bioactive glasses (MBGs) candidates haemostasis. Previously made Tantalum-containing MBG (Ta-MBG) powders' compositions were fabricated as electrospun fibres applications present study. The address challenges associated with powder form: difficult compress without gauze, getting washed away...
Abstract Many biotechnologies require direct contact between biomolecules and (semi)solid substrates. However, little information is available regarding site‐directed covalent surface immobilization of heterodimeric proteins. Integrins are heterodimeric, conformationally dependent membrane adhesion‐receptors, which important in the (patho)biology almost all human diseases. In this study, a biomimetic‐platform developed for oriented integrin heterodimers onto most hydroxyl bearing surfaces,...
Aspirin (ASA) therapy is proven to be effective in preventing adverse cardiovascular events; however, up 30% of patients are non-sensitive their prescribed ASA dosage. In this pilot study, we demonstrated, for the first time, how non-sensitivity can diagnosed using Plateletworks
Background: Physiologically resting platelets circulate in the blood discoid shape.Upon vascular injury or during thrombosis, become activated, extend filopodia, spread and expand surface area, aggregate form thrombi, which relies on dynamic F-actin disassembly reassembly.Although several actin-binding proteins have been identified, largely remain unknown.L-plastin, an actin bundling protein, has not studied platelets.It is predicted with two C-terminal binding domains that can cross-link...