A5100689759- Single-cell and spatial transcriptomics
- Natural Antidiabetic Agents Studies
- Lipoproteins and Cardiovascular Health
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Gene Regulatory Network Analysis
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Immune cells in cancer
- Evolutionary Algorithms and Applications
- Phytochemicals and Antioxidant Activities
- Fatty Acid Research and Health
- Immune Response and Inflammation
- MicroRNA in disease regulation
- NF-κB Signaling Pathways
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Machine Learning in Healthcare
- Diet, Metabolism, and Disease
- Cardiovascular Health and Risk Factors
- Cholesterol and Lipid Metabolism
- Artificial Intelligence in Healthcare and Education
- Corneal Surgery and Treatments
- Liver Disease Diagnosis and Treatment
- Reinforcement Learning in Robotics
- Adversarial Robustness in Machine Learning
Sun Yat-sen University
2011-2024
China International Science and Technology Cooperation
2024
Shantou University
2023-2024
Aerospace Testing (United States)
2022
Gansu Provincial Hospital
2021
XinHua Hospital
2020
Shanghai Jiao Tong University
2020
Shandong University
2019
Peking University
2013-2016
Peking University People's Hospital
2013-2016
Background: S-adenosylhomocysteine (SAH) is a risk factor of cardiovascular disease; inhibition SAH hydrolase (SAHH) results in accumulation and induces endothelial dysfunction atherosclerosis. However, the effect mechanism SAHH atherosclerotic calcification still unclear. We aimed to explore role calcification. Methods: The relationship between was investigated patients with coronary Different vivo genetic models were used examine deficiency on Human aortic murine vascular smooth muscle...
Our previous studies have demonstrated that anthocyanin extract from black rice (AEBR) inhibits atherosclerosis. Whether dietary AEBR supplementation can affect platelet function, an important factor in the pathogenesis of cardiovascular diseases, remains unclear. The aim present study is to explore effects and mechanisms on function lipid profile dyslipidemic rats. We herein thromboxane A2, thrombogenic ratio A2 prostacyclin, serum calmodulin, soluble P-selectin were significantly decreased...
BACKGROUND: Cyanidin-3-O-β-glucoside (Cy-3-g)-rich foods have been reported to inhibit the onset of obesity, but whether pure anthocyanin supplementation affects obesity remains uncertain. RESULTS: Cy-3-g significantly reduced accumulation fat in visceral adipose and liver tissues, plasma triglyceride levels. Furthermore, adenosine monophosphate (AMP)-activated protein kinase phosphorylation (pAMPK) skeletal muscle were increased by consumption. This was followed activation lipoprotein...
Abstract: Anthocyanins are naturally occurring compounds that impart color to fruits, vegetables, and plants. This study aims optimize the microwave‐assisted extraction (MAE) conditions of anthocyanins from mulberry ( M. atropurpurea Roxb.) using response surface methodology (RSM). A Box–Behnken experiment was employed in this regard. Methanol concentration, microwave power, time were chosen as independent variables. The optimized MAE follows: 59.6% acidified methanol, 425 W 25 (v/w)...
Recent studies have shown the role of miRNAs in macrophage reverse cholesterol transport and atherogenesis. We hypothesized that coenzyme Q10 (CoQ10) may increase by regulating miRNA expression contributes to prevention atherosclerosis.CoQ10 treatment suppressed oxidized low-density lipoprotein-induced foam cell formation ameliorating binding activator protein-1 putative promoter region miR-378 primary transcript, thus decreasing level enhancing ATP-binding cassette transporter G1-mediated...
Scope Although previous studies have shown that consumption of anthocyanin extract from plant foods reduces hypercholesterolemia and the severity atherosclerosis in different animal models, mechanisms these actions remained unclear. This study investigated whether pure inhibit development reduce apolipoprotein E ( A po )‐deficient mice through enhancement fecal bile acid excretion, a critical pathway for eliminating circulation cholesterol body. Methods results Five‐week‐old male ‐deficient...
The proatherogenic effect of low-density lipoprotein cholesterol (LDL-C) and antiatherogenic high-density (HDL-C) have been confirmed in general population. But controversy arises among coronary artery disease (CAD) patients. goal this study was to identify the association different lipid measurements with CAD prognosis. cohort included 1916 patients who were 40–85 years age. Cox proportional hazards regression models used estimate baseline 6 factors 3 ratios all-cause cardiovascular (CVD)...
We previously found that miR-10b inhibits cholesterol efflux from thioglycollate-elicited mouse peritoneal macrophages through repressing ATP binding cassette transporter (ABCA1). Herein, we deciphered the mechanism underlying macrophage expression and role of in atherosclerosis.MiR-10b was increased arteries with advanced but not early atherosclerotic plaques ApoE-/- mice. Free cholesterol-induced apoptotic cells (FC-AM) promoted resident (RPM) by up-regulation Twist1/2 a Mer receptor...
Endothelial cells (ECs) with senescence-associated secretory phenotypes (SASP) have been identified as a key mechanism of aging that contributes to various age-related kidney diseases. In this study, we used single-cell RNA sequencing (scRNA-seq) create transcriptome atlas murine renal ECs and identify transcriptomic changes occur during aging. We seven different subtypes ECs, glomerular angiogenic being the most affected by senescence. confirmed our scRNA-seq findings using double...
Scope Since protocatechuic acid exerts an atheroprotective role, we investigated how chicory (Cichorium intybus L. var. foliosum, Belgian endive) rich in acid, a typical vegetable Mediterranean diet, affects preestablished atherosclerosis progression. Methods and results Apolipoprotein E‐deficient mice fed AIN diets containing 0.5% freeze‐dried for 10 weeks displayed reduction lesion size with concomitant improvement stability indicated by fewer macrophages more collagen content. Chicory...
Replenishing the retina with retinal pigment epithelial (RPE) cells derived from pluripotent stem (PSCs) has great promise for treating degenerative diseases, but it is limited by poor cell survival and integration in vivo. Herein, porcine acellular sclera uvea extracellular matrix (ECM) their counterpart hydrogels are developed, effects on biological behavior of human induced (hiPSC)-derived RPE (hiPSC-RPE) embryoid body (hiPSC-EB) differentiation investigated. Both ECM have excellent...
Virus-like particles (VLP) are a promising tool for intracellular gene delivery, yet their potential in ocular therapy remains underexplored. In this study, we bridged knowledge gap by demonstrating the successful generation and application of vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped mouse PEG10 (MmPEG10)-VLP intraocular mRNA delivery. Our findings revealed that PEG10-VLP can efficiently deliver GFP to adult retinal pigment epithelial cell line-19 (ARPE-19) cells, leading...
Background Large language models (LLMs) demonstrated advanced performance in processing clinical information. However, commercially available LLMs lack specialized medical knowledge and remain susceptible to generating inaccurate Given the need for self-management diabetes, patients commonly seek information online. We introduce Retrieval-augmented Information System Enhancement (RISE) framework evaluate its enhancing provide accurate responses diabetes-related inquiries. Objective This...
Protein disulfide isomerase (PDI, EC 5.3.4.1) is a chaperone and catalyzes the formation rearrangement of bonds in proteins. Domain c-(463-491), containing 18 acidic residues, an interesting important C-terminal extension PDI. In this study, PDI mutant abb'a', which domain c truncated, was used to investigate relationship between structure function. Reactivation light-scattering experiments show that both wild-type abb'a' interact with lactate dehydrogenase (LDH, 1.1.1.27), tends...
<h3>Objective</h3> Epidemiological evidence suggests that different lengths of carbon chains might predict cardiovascular disease (CVD) events differently. However, little data exist concerning the effects specific types monounsaturated fatty acids (MUFAs) stratified by chain length. Therefore, study aimed to explore whether associations long-chain MUFAs (LC-MUFAs: 16:1n-7 and 18:1n-9) very (VLC-MUFAs: 20:1n-9, 22:1n-9 24:1n-9) with mortality were among patients coronary artery (CAD)....
Abstract Apoptotic-like phase is an essential step in thrombopoiesis from megakaryocytes. Anthocyanins are natural flavonoid pigments that possess a wide range of biological activities, including protection against cardiovascular diseases and induction tumour cell apoptosis. We investigated the effects underlying mechanisms cyanidin-3-o-β-glucoside (Cy-3-g, major bioactive compound anthocyanins) on apoptosis human primary megakaryocytes Meg-01 line vitro. found Cy-3-g dose-dependently...
Endothelial cell (EC) function declines with age and contributes to the development of many vascular-related disease processes. Currently, effects aging on molecular regulatory mechanisms liver ECs have not been fully elucidated. Here, we employed single-cell RNA sequencing map transcriptome analyzed their relationship aging. We identified 8 different EC subtypes, interestingly, 2 which were specially expressed in aged mice namely capillary (Aged ECs) pro-inflammation (Proinfla.ECs). Double...
Purpose: Corneal epithelial homeostasis is maintained by coordinated gene expression across distinct cell populations, but the regulatory programs underlying this cellular diversity remain to be characterized. Here we applied single-cell multi-omics analysis delineate profile of mouse corneal cells under normal homeostasis. Methods: Single isolated from cornea epithelium (with marginal conjunctiva) adult mice were subjected scRNA-seq and scATAC-seq using 10×Genomics platform. Cell types...
Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to differentiate into osteoblasts or adipocytes, and an imbalance between adipogenesis osteogenesis causes age-related bone loss. In this study, we determined influence of tumor necrosis factor receptor-associated 3 (TRAF3) on senescence osteoblastic adipocytic differentiation rat BMSCs. TRAF3 expression increased during osteogenic but decreased BMSCs, compared with day 0 cultures, 14, differences were significant....
Patients with Graves' disease (GD) can develop ophthalmopathy (GO), but the underlying pathological mechanisms driving this development remain unclear. In our study, which included patients GD and GO, we utilized single-cell RNA sequencing (scRNA-seq) multiplatform analyses to investigate CD169
Abstract Background Aging is associated with significant structural and functional changes in the spleen, leading to immunosenescence, yet detailed effects on splenic vascular endothelial cells (ECs) their immunomodulatory roles are not fully understood. In this study, a single-cell RNA (scRNA) atlas of EC transcriptomes from young aged mouse spleens was constructed reveal age-related molecular changes, including increased inflammation reduced development also potential interaction between...