A5100418334- Pluripotent Stem Cells Research
- Angiogenesis and VEGF in Cancer
- Algal biology and biofuel production
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- RNA modifications and cancer
- Plant Virus Research Studies
- Proteoglycans and glycosaminoglycans research
- Cleft Lip and Palate Research
- Phytochemical compounds biological activities
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Marine and coastal ecosystems
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Dental Radiography and Imaging
- Glycosylation and Glycoproteins Research
- Orthodontics and Dentofacial Orthopedics
- dental development and anomalies
- AI in cancer detection
- Insect and Pesticide Research
First Affiliated Hospital of Henan University
2024
University Medical Center Groningen
2024
University of Groningen
2024
Nanjing Agricultural University
2024
Shandong University
2016-2023
Wenzhou Medical University
2023
First Affiliated Hospital of Wenzhou Medical University
2023
Nanjing Forestry University
2022
Xuzhou Medical College
2018-2022
Beijing University of Chinese Medicine
2022
Abstract Background Plant microbiota contributes to plant growth and health, including enhancing resistance various diseases. Despite remarkable progress in understanding diseases plants, the precise role of rhizosphere watermelon against soil-borne remains unclear. Here, we constructed a synthetic community (SynCom) 16 core bacterial strains obtained from grafted plants. We further simplified SynCom investigated bacteria with synergistic interactions promoting through simple community....
The DEAD-box-protein DDX5 is an ATP-dependent RNA helicase that frequently overexpressed in various cancers and acts as a transcriptional co-activator of several transcription factors, including β-catenin. reported to be involved cancer progression by promoting cell proliferation epithelial-mesenchymal transition. However, the clinical significance biological role non-small-cell lung (NSCLC) remain largely unknown. In this study, we examined expression NSCLC samples, investigated its...
Diabetes is a metabolic disease with high incidence and serious harm to human health. Islet β-cell function defects can occur in the late stage of type 1 diabetes 2 diabetes. Studies have shown that stem cell promising new approach bioengineering regenerative medicine. In study differentiation, three-dimensional (3D) culture more capable mimicking microenvironment growth vivo than two-dimensional (2D) culture. The natural contact between cells cells, extracellular matrix regulate development...
Multidrug resistance is one of the major causes limiting efficacy chemotherapeutic agents used to control osteosarcoma. protein 1 (MDR1 or ABCB1) was considered play a critical role in multidrug resistance. Agents from traditional Chinese medicines (TCMs) have great potential prevent onset delay progression carcinogenic process, and also enhance mainstream antitumor agents. Herein, we investigated effect mechanism icariin human osteosarcoma doxorubicin (DOX)-resistant cell line MG-63/DOX. In...
Aurantiochytrium limacinum has received attention because of its abundance polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). DHA is synthesized through the polyketide synthase (PKS) pathway in A. limacinum. The related enzymes PKS are mainly expressed by three gene clusters, called pks1, pks2 and pks3. In this study, full-length pks3 was obtained polymerase chain reaction amplification Genome Walking technology. Based on a domain analysis deduced amino sequence...
Transcription factor FoxA1 plays a critical role during embryonic development and is activated retinoic acid (RA)-induced neural differentiation of pluripotent P19 embryonal carcinoma cells at the early stage, which marked by decreased expression Nanog increased stem cell marker Nestin. To further understand how mediates differentiation, we have overexpressed through an adenovirus vector in identified that neurogenesis-related sonic hedgehog (Shh) gene directly FoxA1. Knockdown results...
The well known and most important function of nucleoli is ribosome biogenesis. However, the nucleolus showed delayed development malfunction in somatic cell nuclear transfer (NT) embryos. Previous studies indicated that nearly half rRNA genes (rDNA) cells were inactive not transcribed. We compared rDNA methylation level, active nucleolar organizer region (NORs) numbers, proteins (upstream binding factor (UBF), nucleophosmin (B23)) distribution, nucleolar-related gene expression three...
Schisandrin A reversed chemoresistance in doxorubicin-induced MG-63 subline by inhibiting NF-kappaB-mediate expression of P-gp.
Real-time reverse transcription quantitative polymerase chain reaction (qPCR) has become the most frequently used system for studies of gene expression. Manystudies have provided reliable evidence that levels reference genes are not constant at different developmental stages and in experimental conditions. However, suitable which stably expressed polyploid preimplantation embryos yet been identified. Therefore, it is critical to verify candidate analyze expression accurately both diploid...
Abstract We hypothesized that microRNA binding site single nucleotide polymorphisms (SNPs) in fibroblast growth factors ( FGFs ) and their receptor genes FGFRs may affect mRNA interactions are thereby associated with susceptibility of non-syndromic orofacial cleft (NSOC). Ten SNPs among the FGF FGFR were selected associations NSOC investigated a case-control study 602 patients 605 healthy controls. FGF2 /rs1048201, FGF5 /rs3733336 FGF9 /rs546782 showed suggestive association susceptibility....
Acute lung injury (ALI) is associated with an increased incidence of respiratory diseases, which are devastating clinical disorders high global mortality and morbidity. Evidence confirms that fibroblast growth factors (FGFs) play key roles in mediating ALI. Mice were treated LPS (lipopolysaccharide: 5 mg/kg, intratracheally) to establish vivo ALI model. Human epithelial BEAS-2B cells cultured a corresponding medium used mimic the model vitro. In this study, we characterized FGF10...
Abstract Reprogramming of somatic cells was induced by ES cell‐free extract. The system relied on the transient uptake regulatory components from a nuclear and cytoplasmic extract derived nucleus reversibly permeabilized NIH3T3 cell. were streptolysin O (SLO). extracts prepared repeatedly freezing thawing in liquid nitrogen. After incubation for 1 hr, resealed CaCl 2 continually cultured weeks to assess expression cell specific markers. As we observed using FACS fluorescence microscope,...
Our previous study has shown that the transcription factor Krüppel-like 7 (KLF7) promotes peripheral nerve regeneration and motor function recovery after spinal cord injury. KLF7 also participates in traumatic brain injury, but its regulatory mechanisms remain poorly understood. In present study, an HT22 cell model of injury was established by stretch oxygen-glucose deprivation. These cells were then transfected with adeno-associated virus carrying (AAV-KLF7). The results revealed that,...