A5072095183- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Ocular Surface and Contact Lens
- Immune cells in cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Ophthalmology and Visual Impairment Studies
- Intraocular Surgery and Lenses
- Fibroblast Growth Factor Research
- Cell Adhesion Molecules Research
- Corneal Surgery and Treatments
- Ocular Oncology and Treatments
- Proteoglycans and glycosaminoglycans research
- Retinal and Optic Conditions
- Dermatologic Treatments and Research
- Single-cell and spatial transcriptomics
- Corneal surgery and disorders
- Ergonomics and Musculoskeletal Disorders
- Immunotherapy and Immune Responses
- Ocular Diseases and Behçet’s Syndrome
- Retinal Development and Disorders
- Ophthalmology and Eye Disorders
- T-cell and B-cell Immunology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Kruppel-like factors research
Sun Yat-sen University
2014-2025
Key Laboratory of Guangdong Province
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
State Key Laboratory of Ophthalmology
2022
Hainan Medical University
2020
Fujian Medical University
2006
Union Hospital
2006
Although VEGF-B was discovered as a VEGF-A homolog long time ago, the angiogenic effect of remains poorly understood with limited and diverse findings from different groups. Notwithstanding, drugs that inhibit together other VEGF family members are being used to treat patients various neovascular diseases. It is therefore critical have better understanding underlying mechanisms. Using comprehensive in vitro vivo methods models, we reveal here for first an unexpected surprising function...
To investigate the effect of pirfenidone, a novel antifibrotic agent, on proliferation, migration, and collagen contraction human Tenon's fibroblasts (HTFs).After treatment HTFs with cell proliferation was measured by MTT assay. Cell migration investigated scratch Contractility evaluated in fibroblast-populated gels. viability determined trypan blue exclusion The expression TGF-beta1, -beta2, -beta3 estimated RT-PCR, Western blot, immunofluorescence analyses.Pirfenidone induced significant...
Purpose.: To investigate whether topical administration of pirfenidone eye drops could be used to prevent postoperative scarring in a rabbit model experimental glaucoma filtration surgery. Methods.: In randomized, controlled, masked-observer study, 40 rabbits underwent trabeculectomy the right eyes and randomly received 0.1% or 0.5% pirfenidone, perioperative mitomycin C (0.25 mg/mL), no treatment. Bleb characteristics functions were evaluated over period 4 weeks. The animals killed on days...
The purpose of the study was to describe prevalence, severity, and associated risk factors for pterygium in a population-based sample rural residents Bai minority population Dali, China.A survey Chinese Nationality aged ≥50 years from randomly selected block groups southwestern China conducted. A clinical examination by experienced ophthalmologists carried out, presence diagnosed at examination. Pterygium graded clinically slit lamp Questionnaires were conducted on factors.From total 2742...
Background Posterior capsular opacification (PCO) is a common complication of cataract surgery. Transforming growth factor-β2 (TGF-β2) plays important roles in the development PCO. The existing pharmacological treatments are not satisfactory and can have toxic side effects. Methodologies/Principal Findings We evaluated effect pirfenidone on proliferation, migration epithlial-mesenchymal transition human lens epithelial cell line SRA01/04 (HLECs) vitro. After treatment with 0, 0.25, 0.5 mg/ml...
Significance Despite being discovered a long time ago, the functional properties of VEGF-B remain poorly understood. However, several clinical treatments use drugs that target and other VEGF family members. It is therefore crucial to gain deeper insights into function underlying mechanisms. Here, we found has potent antioxidative functions, making it member show such property. We further identified critical downstream effector VEGF-B, Gpx1, through which protects against retinal...
Purpose: Eyelid infiltrative basal cell carcinoma (iBCC) is the most common malignant tumor affecting ocular adnexa, but studies on metabolic changes within its microenvironment and heterogeneity at invasive area are limited. This study aims to analyze differences among iBCC types using single-cell spatial metabolomics analysis examine environment area. Methods: Single-cell transcriptomic data of human (BCC) were clustered visualized Uniform Manifold Approximation Projection. Metabolic...
Lacrimal irrigation is a fundamental skill for diagnosing and managing lacrimal diseases. This study evaluates two techniques introduces haptic-visual integrated self-serve training model to enhance acquisition among novice ophthalmology residents. Ninety-two residents were randomized into Group A (n = 47) B 45). Both groups completed an 8-hour program comprising theoretical instruction, demonstrations, hands-on practice. provided feedback refine the model, which was subsequently implemented...
Purpose The aim of the study was to assess levels health literacy and computer skills in Chinese patients with cataract, their impact on doctor-patient relationship. Methods We undertook a cross-sectional cataract scheduled for extraction procedures Guangdong Province, China. Generic assessed using 3 established screening questions. Adequate determined if had used routinely search engines Internet. Socio-demographic measures (e.g., age, sex, education) were obtained from standardized...
Significance Caveolin-1 (Cav-1) is a major structural protein of caveolae found in cell membranes and critical for numerous cellular functions. However, it remains unclear whether Cav-1 plays role ocular neovascularization, cause blindness. In this study, we that the gene deletion exacerbates cavtratin, permeable peptide mimicking function, inhibits neovascularization by targeting multiple components angiogenesis. Importantly, combined administration cavtratin anti–VEGF-A more effectively,...
Purpose To develop and validate a Chinese version of the Catquest-9SF questionnaire in cataract population. Methods The Questionnaire was translated back into Chinese. Preoperative patients were recruited at tertiary eye hospital their demographic information visual acuity documented. Psychometric properties Catquest-9SF, including ordered thresholds, ability to distinguish between different strata person ability, absence misfitting items, unidimentionality, differential item functioning...
Few reports described efficient transfection in the trabecular meshwork (TM) vivo. In present study, we investigated distribution of cy3-labeled siRNAs after giving injection into anterior chamber (AC) and explored use RhoA siRNA (siRhoA) to modulate intraocular pressure (IOP) through downregulation gene protein expression. Cy3-labeled were injected AC investigate distribution. addition, siRhoA was applied normal DEX-induced elevated IOP mice. The detected at 1d post-injection (PI) using...
To investigate the relationship between preoperative expectations and actual postoperative outcomes of visual function (VF) among patients undergoing first eye cataract surgery.A longitudinal study 182 from hospitals in urban Southern China were surveyed prior to surgery 3 month after regarding their preoperative, expected VF for each items on Catquest-9SF satisfaction with surgery. In addition, detailed clinical data collected preoperatively postoperatively.The majority had high most cases...
Dendritic cells (DCs) are critical for pathogen recognition and Ag processing/presentation. Human monocyte-derived DCs (moDCs) have been extensively used in experimental studies DC-based immunotherapy approaches. However, the extent of human moDC peripheral heterogeneity their interrelationship remain elusive. In this study, we performed single-cell RNA sequencing moDCs blood DCs. We identified seven subtypes within moDCs: five corresponded to type 2 conventional (cDC2s), other two were...
Behect's disease is a chronic vasculitis characterized by complex multi-organ immune aberrations. However, comprehensive understanding of the gene-regulatory profile peripheral autoimmunity and diverse responses across distinct cell types in Behcet's (BD) still lacking. Here, we present multi-omic single-cell study 424,817 cells BD patients non-BD individuals. This maps chromatin accessibility gene expression same biological samples, unraveling vast cellular heterogeneity. We identify...
Objectives: We aimed to investigate whether CYP2D6 polymorphisms were associated with the intraocular pressure (IOP)-lowering effect and systemic complications (especially bradycardia) of ophthalmic timolol. Methods: One hundred twenty-three primary open-angle glaucoma subjects (123 eyes) treated 0.5% aqueous formulations IOP heart rate measured before after timolol administration. DNA was extracted from venous leukocytes all subjects. Two single-nucleotide (SNPs) CYP2D6, for example,...
Platelet-derived growth factor-D (PDGF-D) is highly expressed in immune cells. However, the potential role of PDGF-D system remains thus far unclear. Here, we reveal a novel function activating both classical and alternative complement pathways that markedly increase chemokine cytokine responses to promote macrophage polarization. Pharmacological targeting C3a receptor using SB290157 alleviated PDGF-D-induced neuroinflammation by blocking polarization inhibited pathological choroidal...
Patients with Graves' disease (GD) can develop ophthalmopathy (GO), but the underlying pathological mechanisms driving this development remain unclear. In our study, which included patients GD and GO, we utilized single-cell RNA sequencing (scRNA-seq) multiplatform analyses to investigate CD169