A5103135256- Plant Gene Expression Analysis
- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Lignin and Wood Chemistry
- Hemostasis and retained surgical items
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Platelet Disorders and Treatments
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Gene expression and cancer classification
- Total Knee Arthroplasty Outcomes
- Dental Implant Techniques and Outcomes
- RNA Interference and Gene Delivery
- Telemedicine and Telehealth Implementation
- Marine Toxins and Detection Methods
- Single-cell and spatial transcriptomics
- Scientific Computing and Data Management
- Genomics, phytochemicals, and oxidative stress
- Antiplatelet Therapy and Cardiovascular Diseases
- Immunotherapy and Immune Responses
- Atherosclerosis and Cardiovascular Diseases
- Soil and Unsaturated Flow
Canadian Blood Services
2007-2024
University of British Columbia
2003-2024
University of British Columbia Hospital
2024
Chungnam National University
2012-2022
North Carolina State University
2009-2021
Stanford University
2021
VA Palo Alto Health Care System
2021
St. Michael's Hospital
2018
Dankook University
2018
Southerners on New Ground
2014
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in formation Populus trichocarpa has at least 21 genes, encoding enzymes that mediate 37 reactions on 24 metabolites, leading to affecting properties. We perturb these genes integrate transcriptomic, proteomic, fluxomic phenomic data 221 lines...
Abstract Platelet αIIbβ3 integrin and its ligands are essential for thrombosis hemostasis, play key roles in myocardial infarction stroke. Here we show that apolipoprotein A-IV (apoA-IV) can be isolated from human blood plasma using platelet β3 integrin-coated beads. Binding of apoA-IV to platelets requires activation integrin, the direct apoA-IV-αIIbβ3 interaction detected a single-molecule Biomembrane Force Probe. We identify aspartic acids 5 13 at N-terminus required binding which is...
As a step toward predictive modeling of flux through the pathway monolignol biosynthesis in stem differentiating xylem Populus trichocarpa, we discovered that two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact vivo vitro to form heterotetrameric protein complex. This conclusion is based on laser microdissection, coimmunoprecipitation, chemical cross-linking, bimolecular fluorescence complementation, mass spectrometry. The tetramer composed three subunits one 4CL5. 4CL5...
Chemical imaging by confocal Raman microscopy has been used for the visualization of cellulose and lignin distribution in wood cell walls. Lignin reduction can be achieved by, example, transgenic suppression a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use to compare lignification wild type lignin-reduced 4CL Populus trichocarpa stem with spatial resolution that is sub-μm. Analyzing bands spectral region between 1,600 1,700 cm−1, differences signal intensity...
Abstract 4-Coumaric acid:coenzyme A ligase (4CL) is involved in monolignol biosynthesis for lignification plant cell walls. It ligates coenzyme (CoA) with hydroxycinnamic acids, such as 4-coumaric and caffeic into hydroxycinnamoyl-CoA thioesters. The ligation ensures the activated state of acid reduction monolignols. In Populus spp., it has long been thought that one monolignol-specific 4CL involved. Here, we present evidence two 4CLs, Ptr4CL3 Ptr4CL5, trichocarpa. ortholog reported many...
The B7-H3 protein, encoded by the CD276 gene, is a member of B7 family proteins and transmembrane glycoprotein. It highly expressed in various solid tumors, such as lung breast cancer, has been associated with limited expression normal tissues poor clinical outcomes across different malignancies. Additionally, plays crucial role anticancer immune responses. Antibody-drug conjugates (ADCs) are promising therapeutic modality, utilizing antibodies targeting tumor antigens to selectively...
Resveratrol at high concentrations (50–100 μmol/L) is known to induce cell death in leukemia cells. Here, we investigated whether pinosylvin, a resveratrol analogue, induced Cell was found be markedly elevated by 50‐ 100‐μmol/L pinosylvin THP‐1 and U937 It also shown that caspase‐3 activation, flip‐flop of phosphatidylserine, LC3‐II accumulation, LC3 puncta, p62 degradation both These data indicate pinosylvin‐induced may occur through apoptosis autophagy. In addition, showed down‐regulates...
Co-enzyme A (CoA) ligation of hydroxycinnamic acids by 4-coumaric acid:CoA ligase (4CL) is a critical step in the biosynthesis monolignols. Perturbation 4CL activity significantly impacts lignin content diverse plant species. In Populus trichocarpa , two well-studied xylem-specific Ptr4CLs (Ptr4CL3 and Ptr4CL5) catalyze CoA acid to 4-coumaroyl-CoA caffeic caffeoyl-CoA. Subsequently, 4-hydroxycinnamoyl-CoA:shikimic hydroxycinnamoyl transferases (PtrHCT1 PtrHCT6) mediate conversion Here, we...
The Revised Universal Soil Loss Equation (RUSLE) is a revision of the (USLE). However, changes for each factor USLE have been made in RUSLE which can be used to compute soil loss on areas only where significant overland flow occurs. requires standardized methods satisfy new data requirements estimates movement at particular site by utilizing same factorial approach employed USLE. rainfall erosivity expressed through R-factor quantify effect raindrop impact and reflect amount rate runoff...
Recently we have reported that the ortho-hydroxy-protected aryl sulfate (OHPAS) system can be exploited as a new self-immolative group (SIG) for phenolic payloads. We extended to nonphenolic payloads by simply introducing para-hydroxy benzyl (PHB) spacer. As an additional variation of system, explored benzylsulfonate version OHPAS and found it has two distinct breakdown pathways, cyclization 1,4-elimination, latter which implies para-hydroxy-protected (PHP) (BS) also used alternative SIG....
ABSTRACT The determinants of severe COVID-19 in non-elderly adults are poorly understood, which limits opportunities for early intervention and treatment. Here we present novel machine learning frameworks identifying common rare disease-associated genetic variation, outperform conventional approaches. By integrating single-cell multiomics profiling human lungs to link signals cell-type-specific functions, have discovered validated over 1,000 risk genes underlying across 19 cell types....
α-Synuclein accumulation is implicated in the pathogenesis of neurodegenerative diseases, including Parkinson's disease (PD). Previously, we reported that Fas-associated factor 1 (FAF1), which plays a role PD pathogenesis, potentiates α-synuclein through autophagy impairment dopaminergic neurons. In this study, show KM-819, FAF1-targeting compound, has completed phase I clinical trials, interferes with mouse brain, as well human neuronal cells (SH-SY5Ys). KM-819 suppressed monomeric,...
In this study, a ferroelectric layer was formed on device via plasma enhanced atomic deposition. The used 50 nm thick TiN as upper and lower electrodes, an Hf0.5Zr0.5O2 (HZO) material applied to fabricate metal–ferroelectric–metal-type capacitor. HZO devices were fabricated in accordance with three principles improve their properties. First, the nanolaminate thickness of layers varied. Second, heat treatment performed at 450, 550, 650 °C investigate changes characteristics function...
Blood coagulation FV (Factor V) is activated by thrombin-mediated excision of the B domain, resulting in a non-covalent heterodimer, FVa (activated FV). Previous studies implicated Glu96, Asp102 and Asp111 essential Ca2+-dependent subunit interaction. In present study, E96A, D102A D111A were purified evaluated for function, dissociation metal ion binding. Chromogenic clotting assays presence procoagulant vesicles showed that each variant was inhibited (∼20–40%). further (>90%) after...
Abstract Biomedical studies have become larger in size and yielded large quantities of data, yet efficient data processing remains a challenge. Here we present Trellis, cloud-based task management framework that completely automates the process from ingestion to result presentation, while tracking lineage, facilitating information query, supporting fault-tolerance scalability. Using graph database coordinate state workflows scalable microservice architecture perform bioinformatics tasks,...
피노실빈은 소나무 등에서 흔히 관찰되는 스틸벤노이드이다. 혈관내피세포에서 ~pM에서 ~nM 정도의 낮은 농도의 세포성장, 세포이동, 항염증반응을 유도한다. 그러나 최근에 고농도의 피노실빈이 소 대동맥 내피세포의 세포사를 유발하고 있음이 보고되었으나, 그 자세한 기전이나 경로 연구가 미흡하여 이번 연구에서 고농도 피노실빈의 세포사 유발 경로를 규명하기 위한 실험을 수행하였다. caspase-3 절단, 포스파티딜 세린의 플립플롭, 핵 분절 등을 유도하는 것으로 보아 세포사멸을 통한 유발함을 알았다. 또한 혈청기아나 100 μM etoposide에 의하여 촉발하는 활성/핵분절이 추가적으로 증가하는 현상이 관찰되었다. 이는 일으키는 세포사멸은 의해 일어나는 세포사멸과는 다른 경로로 진행됨을 의미하므로 피노실빈에 촉발되는 세포신호전달을 탐색한 결과, JNK와 eNOS가 관여함을 두 신호전달 물질 중에 어느 것이 피노실빈 유도 세포사에 중요한 지를 알기 추가적인 진행하였다....
Abstract The strawberry Fra a 1 proteins belong to the class 10 Pathogenesis-Related (PR-10) superfamily. In strawberry, large number of members have been identified, but only limited is expressed in fruits. this organ, 1.01 and 1.02 are most abundant green red fruits, respectively, however, their function remains unknown. To know we generated transgenic lines that silence gene, performed metabolomics, RNA-Seq, hormonal assays. Previous studies associated fruit color, analysis anthocyanins...
Congenital factor V (FV) deficiency is a rare clotting disorder affecting ∼1 in 1,000,000, with bleeding severity that ranges broadly for poorly understood reasons.
마늘은 전통적으로 cycloalliin을 포함한 기능성 식품으로 널리 알려져 있다. 본 연구를 통해 혈관계에서 발효마늘추출물이 어떤 효능을 갖는지 알아보았다. 먼저, 내피세포의 단핵구 부착을 억제하는 것을 관찰하였다. 농도 변화에 따른 실험결과, 발효마늘추출물을 0.1 또는 <TEX>$1{\mu}g/ml$</TEX> 농도로 전처리 하면, LPS에 의하여 촉발되는 소 대동맥 내피세포에 달라붙는 THP-1의 부착이 억제되는 확인하였다. 단핵구의 내피세포 염증반응 초기단계에 진행되는 과학적 근거를 고려하면 이와 같은 결과는 혈관 염증을 저해한다고 볼 수 또한 세포신호전달물질의 활성변화를 관찰한 결과, 내피세포에서 eNOS와 Akt의 활성을 유도함을 알았다. eNOS의 인산화 반응은 처리한지 10분 후에 최대로 상승되었으며, 유사하게 Akt도 처리하고 후에서 반응이 상승되었다. 산화질소 양 변화 측정에서도 발효마늘추출물은 산화질소의 생성을 상승시켰다. 한편, 저해하는 억제제 처리는...