A5002104946- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Biochemical and biochemical processes
- Fermentation and Sensory Analysis
- Bioenergy crop production and management
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Advanced Cellulose Research Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Advanced Proteomics Techniques and Applications
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Enzyme-mediated dye degradation
- Marine Sponges and Natural Products
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Cancer Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Plant Parasitism and Resistance
- Microbial Natural Products and Biosynthesis
Zhejiang A & F University
2024-2025
Research Institute of Forestry
2015-2024
Chinese Academy of Forestry
2015-2024
Beijing Forestry University
2024
State Key Laboratory of Tree Genetics and Breeding
2024
South China Agricultural University
2021
Michigan Technological University
2021
Yibin University
2021
Hebei University of Engineering
2021
Northwest A&F University
2021
Laccases, as early 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support this hypothesis has been elusive due functional redundancy laccase genes. An Arabidopsis double mutant demonstrated involvement laccases lignin biosynthesis. We previously identified a subset genes be targets microRNA (miRNA) ptr-miR397a Populus trichocarpa . To elucidate roles and its targets, we characterized gene family 49 models, which 29 predicted ptr-miR397a....
As a step toward comprehensive description of lignin biosynthesis in Populus trichocarpa, we identified from the genome sequence 95 phenylpropanoid gene models 10 protein families encoding enzymes for monolignol biosynthesis. Transcript abundance was determined all genes xylem, leaf, shoot and phloem using quantitative real-time PCR (qRT-PCR). We 23 that most probably encode during wood formation. Transcripts 18 are abundant specific to differentiating xylem. found evidence suggesting...
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in formation Populus trichocarpa has at least 21 genes, encoding enzymes that mediate 37 reactions on 24 metabolites, leading to affecting properties. We perturb these genes integrate transcriptomic, proteomic, fluxomic phenomic data 221 lines...
Abstract Wood formation involves consecutive developmental steps, including cell division of vascular cambium, xylem expansion, secondary wall (SCW) deposition, and programmed death. In this study, we identified PagMYB31 as a coordinator regulating these processes in Populus alba × glandulosa built PagMYB31-mediated transcriptional regulatory network. mutation caused fewer layers cambial cells, larger fusiform initials, ray vessels, fiber enhanced SCW thickening, showing that positively...
We established a predictive kinetic metabolic-flux model for the 21 enzymes and 24 metabolites of monolignol biosynthetic pathway using Populus trichocarpa secondary differentiating xylem. To establish this model, comprehensive study was performed to obtain reaction inhibition parameters all based on functional recombinant proteins. A total 104 Michaelis-Menten 85 were derived from these enzymes. Through mass spectrometry, we obtained absolute quantities in This extensive experimental data...
The hydroxylation of 4- and 3-ring carbons cinnamic acid derivatives during monolignol biosynthesis are key steps that determine the structure properties lignin. Individual enzymes have been thought to catalyze these reactions. In stem differentiating xylem (SDX) Populus trichocarpa, two 4-hydroxylases (PtrC4H1 PtrC4H2) a p-coumaroyl ester 3-hydroxylase (PtrC3H3) involved in Here we present evidence hydroxylases interact, forming heterodimeric (PtrC4H1/C4H2, PtrC4H1/C3H3, PtrC4H2/C3H3)...
Secondary Wall-Associated NAC Domain 1s (SND1s) are transcription factors (TFs) known to activate a cascade of TF and pathway genes affecting secondary cell wall biosynthesis (xylogenesis) in Arabidopsis poplars. Elevated SND1 transcriptional activation leads ectopic xylogenesis stunted growth. Nothing is about the upstream regulators SND1. Here we report discovery stem-differentiating xylem (SDX)-specific alternative splice variant, PtrSND1-A2(IR), that acts as dominant negative network...
Wood remains the world's most abundant and renewable resource for timber pulp is an alternative to fossil fuels. Understanding molecular regulation of wood formation can advance engineering more efficient material energy productions. We integrated a black cottonwood (Populus trichocarpa) wood-forming cell system with quantitative transcriptomics chromatin binding assays construct transcriptional regulatory network (TRN) directed by key transcription factor (TF), PtrSND1-B1 (secondary...
High-throughput single-cell RNA sequencing (scRNA-seq) has advantages over traditional RNA-seq to explore spatiotemporal information on gene dynamic expressions in heterogenous tissues. We performed Drop-seq, a method for the dropwise sequestration of single cells sequencing, protoplasts from differentiating xylem Populus alba × glandulosa. The scRNA-seq profiled 9,798 cells, which were grouped into 12 clusters. Through characterization differentially expressed genes each cluster and situ...
Abstract To date, little is known about the evolution of fern genomes, with only two small genomes published from heterosporous Salviniales. Here we assembled genome Alsophila spinulosa , as flying spider-monkey tree fern, onto 69 pseudochromosomes. The remarkable preservation synteny, despite resulting an ancient whole-genome duplication over 100 million years ago, unprecedented in plants and probably speaks to uniqueness ferns. Our detailed investigations into stem anatomy lignin...
As a step toward predictive modeling of flux through the pathway monolignol biosynthesis in stem differentiating xylem Populus trichocarpa, we discovered that two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact vivo vitro to form heterotetrameric protein complex. This conclusion is based on laser microdissection, coimmunoprecipitation, chemical cross-linking, bimolecular fluorescence complementation, mass spectrometry. The tetramer composed three subunits one 4CL5. 4CL5...
Significance Wood is a widely used renewable feedstock for industrial production and energy generation. The secondary cell wall (SCW) the major component of wood. Two key transcription factor families, Vascular-Related NAC-Domain (VND) Secondary Wall-Associated NAC Domain (SND), are master gene regulators SCW biosynthesis. However, plants exhibit stunted growth or abnormal development under excess VND SND expression. In this study, we show that two splice variants, PtrVND6-C1 IR PtrSND1-A2 ,...
Summary Adventitious roots occur naturally in many species and can also be induced from explants of some tree including Populus , providing an important means clonal propagation. Auxin has been identified as playing a crucial role adventitious root formation, but the associated molecular regulatory mechanisms need to elucidated. In this study, we examined Pag FBL 1 hybrid poplar ( alba × P . glandulosa clone 84K) homolog Arabidopsis auxin receptor TIR 1, formation poplar. Similar...
Summary Although polyploid plants have larger leaves than their diploid counterparts, the molecular mechanisms underlying this difference (or trait) remain elusive. Differentially expressed genes (DEGs) between triploid and full‐sib poplar trees were identified from two transcriptomic data sets followed by a gene association study among DEGs to identify key leaf growth regulators. Yeast one‐hybrid system, electrophoretic mobility shift assay, dual‐luciferase assay employed substantiate that...
Abstract Activity of the vascular cambium gives rise to secondary xylem for wood formation in trees. The transcription factor WUSCHEL-related HOMEOBOX4 (WOX4) is a central regulator downstream hormone and peptide signaling pathways that maintain cambial activity. However, genetic regulatory network underlying WOX4-mediated at post-transcriptional level remains be elucidated. In this study, we identified ubiquitin receptor PagDA1 hybrid poplar (Populus alba × Populus glandulosa clone 84K) as...
ABSTRACT The biosynthesis of cellulose, lignin, and hemicelluloses in plant secondary cell walls (SCWs) is regulated by a hierarchical transcriptional regulatory network. This network features orthologous transcription factors shared between poplar Arabidopsis, highlighting foundational similarity their genetic regulation. However, knowledge on the discrepant behavior transcriptional‐level molecular mechanisms Arabidopsis remains limited. In this study, we investigated function PagMYB128...