A5018169632- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Sesquiterpenes and Asteraceae Studies
- MicroRNA in disease regulation
- Plant tissue culture and regeneration
- Genetic diversity and population structure
- Plant Gene Expression Analysis
- Food Quality and Safety Studies
- Traditional Chinese Medicine Analysis
- Plant Disease Management Techniques
- Agriculture, Soil, Plant Science
- Horticultural and Viticultural Research
- Soybean genetics and cultivation
- Plant and Fungal Species Descriptions
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Molecular Biology Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Plant and Fungal Interactions Research
- Medicinal Plant Pharmacodynamics Research
- Fermentation and Sensory Analysis
- Nematode management and characterization studies
- Biocrusts and Microbial Ecology
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2024
Shanxi Normal University
2014
Research Institute of Forestry
2007
Chinese Academy of Forestry
2007
Laccases, as early 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support this hypothesis has been elusive due functional redundancy laccase genes. An Arabidopsis double mutant demonstrated involvement laccases lignin biosynthesis. We previously identified a subset genes be targets microRNA (miRNA) ptr-miR397a Populus trichocarpa . To elucidate roles and its targets, we characterized gene family 49 models, which 29 predicted ptr-miR397a....
Terpenoids are the largest class of plant secondary metabolites and have attracted widespread interest.Salvia miltiorrhiza, belonging to most widely distributed genus in mint family, is a model medicinal with great economic value.Diterpenoid tanshinones major lipophilic bioactive components S. miltiorrhiza.Systematic analysis genes involved terpenoid biosynthesis has not been reported date.Searching recently available working draft miltiorrhiza genome, 40 biosynthesis-related were...
Abstract Background Digitalis purpurea is an important ornamental and medicinal plant. There considerable interest in exploring its transcriptome. Results Through high-throughput 454 sequencing subsequent assembly, we obtained 23532 genes, of which 15626 encode conserved proteins. We determined 140 unigenes to be candidates involved cardiac glycoside biosynthesis. It could grouped into 30 families, 29 were identified for the first time D. . 2660 mRNA-like npcRNA (mlncRNA) candidates,...
microRNAs (miRNAs) play vital regulatory roles in many organisms through direct cleavage of transcripts, translational repression, or chromatin modification. Identification miRNAs has been carried out various plant species. However, no information is available for from Panax ginseng, an economically significant medicinal Using the next generation high-throughput sequencing technology, we obtained 13,326,328 small RNA reads roots, stems, leaves and flowers P. ginseng. Analysis these RNAs...
Syzygium buxifolium. Hook. Et Arn.1833 is a member of the Myrtaceae family. This species used in traditional Chinese medicines. It possesses numerous synonyms, reflecting ambiguity its taxonomy. The chloroplast genome has been widely for identification and phylogenetic analysis. Regrettably, there lack information regarding S. Here, we intend to obtain buxifolium resolve classification problems. In particular, utilized Illumina sequencing technology sequence, GetOrganelle assemble, CPGAVAS2...
Abstract Ganoderma lucidum is a white-rot fungus best-known for its medicinal and ligninolytic activities. To discover the underlying genes responsible these activities, we identified characterized natural antisense transcripts (NATs) using strand-specific (ss) RNA-seq data obtained from mycelia, primordia fruiting bodies. NATs were custom pipeline then subjected to functional enrichment differential expression analyses. A total of 1613 cis- 244 trans- sense identified. Mapping GO terms KEGG...
摘要: 实时荧光定量PCR(qRT-PCR)技术具有高灵敏性、高保真性和高特异性, 被广泛应用于基因表达的分析。在数据处理过程中, 选用稳定表达的基因作为内参基因对准确分析实验结果非常关键。以毛果杨(Populus trichocarpa)的不同组织以及锌胁迫下的组培苗为材料, 使用荧光定量PCR方法分析了TUA8、TUB6、ubiquitin、GAPDH、actin、18S rRNA和EF1α 7个看家基因的表达情况。通过geNorm、NormFinder和BestKeeper 3个程序的综合分析, 发现actin、ubiquitin、EF1α和18S rRNA的稳定性较好, 可用作毛果杨基因表达研究的内参基因; 而TUB6在不同组织中稳定性最差; GAPDH在锌胁迫下的组织中稳定性最差, 因此不适宜作为内参基因。毛果杨NAC基因的表达分析, 进一步验证了上述结果。该研究对采用qRT-PCR方法分析毛果杨基因表达过程中内参基因的选择具有指导作用, 同时对揭示NAC基因的功能也有一定的意义。
SummaryFactors affecting the rooting of in vitro shoots were investigated using precocious walnut (Juglans regia L.) cv. Xinzaofeng. In sub-culture was most important step to achieve shoots. Rooting markedly affected by method induction, 3-indole-butyric acid (IBA) concentrations, light conditions, as well shoot quality. rates ≥ 75.4% obtained with an optimised protocol which vigorous sub-cultured 45-times at 20 d intervals. These appeared tender, small leaves. A transfer root induction...
Abstract Background Inula britannica is an important medicinal plant of Asteraceae. Its dry capitulum has been used as a traditional Chinese herbal medicine, which had the functions treating edemas, reduction in nausea, and preventing vomiting. There have not many chloroplast genome studies. Methods results we de novo assembly complete I. . The was characterized compared with other five species. Britannica 150,774 bp long. It showed typical quadripartite structure, containing large...