A5012949324- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Plant Micronutrient Interactions and Effects
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Ginkgo biloba and Cashew Applications
- Aluminum toxicity and tolerance in plants and animals
- Horticultural and Viticultural Research
- Heavy metals in environment
- Lipid metabolism and biosynthesis
- Advanced Cellulose Research Studies
- Heat shock proteins research
- Polysaccharides Composition and Applications
- Neurological Disease Mechanisms and Treatments
- Nuclear Structure and Function
- Lignin and Wood Chemistry
- Fermentation and Sensory Analysis
Qingdao Agricultural University
2019-2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2012-2024
Chinese Academy of Sciences
2006-2024
Shandong Academy of Forestry
2024
Oil Crops Research Institute
2009-2010
Chinese Academy of Agricultural Sciences
2009-2010
Institute of Genetics and Developmental Biology
2006
Mannans are hemicellulosic polysaccharides that considered to have both structural and storage functions in the plant cell wall. However, it is not yet known how mannans function Arabidopsis (Arabidopsis thaliana) seed mucilage. In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed several tissues, including epidermal cells of developing coats. Disruption CSLA2 resulted thinner adherent mucilage halos, although total amount did change compared with wild type....
Ginkgo biloba is a monotypic species native to China with great economic and ecological values. Leaves extract of this tree contains about 24% flavonoids, which are widely used in the pharmaceutical industry. However, flavonoids biosynthesis pathway poorly understood Ginkgo. In study, we comprehensively compared transcriptome metabolite profiles high-flavonoids mutant (ZY1) Anlu1 (control) leaves. A total 122 significantly changed metabolites 1683 differentially expressed genes (DEGs),...
Background Homeodomain-leucine zipper (HD-ZIP) proteins are plant-specific transcriptional factors known to play crucial roles in plant development. Although sequence phylogeny analysis of Populus HD-ZIPs was carried out a previous study, no systematic incorporating genome organization, gene structure, and expression compendium has been conducted model tree species thus far. Principal Findings In this comprehensive HD-ZIP family performed. Sixty-three full-length genes were found genome....
CCCH zinc finger proteins contain a typical motif of three cysteines and one histidine residues serve regulatory functions at all stages mRNA metabolism. In plants, type comprise large gene family represented by 68 members in Arabidopsis 67 rice. These have been shown to play diverse roles plant developmental processes environmental responses. However, this has not studied the model tree species Populus date.
Plant tandem CCCH zinc finger (TZF) proteins play diverse roles in developmental and adaptive processes. Arabidopsis C3H14 has been shown to act as a potential regulator of secondary wall biosynthesis. However, there is lack direct evidence support its functions Arabidopsis. It demonstrated here that homologue C3H15 redundantly regulate formation they additionally function anther development. Plants with double, but not single, T-DNA mutants for or have few pollen grains thinner stem walls...
Summary Wood biomass is mainly made of secondary cell walls, whose formation controlled by a multilevel network. The tandem CCCH zinc finger ( TZF ) proteins involved in plant wall are poorly understood. Two genes, P d C 3 H 17 and 18 , were isolated from opulus deltoides functionally characterized E scherichia coli tobacco, A rabidopsis poplar. predominantly expressed cells developing wood, the they encode targeted to cytoplasmic foci. Transcriptional activation assays showed that MYB...
In plants, the R2R3-MYB gene family contains many pairs of paralogous genes, which play diverse roles in developmental processes and environmental responses. The paper reports characterization 81 Populus genes. Chromosome placement, phylogenetic, motif structure analyses showed that these resulted from multiple types duplications had five different fates. Tissue expression patterns revealed most duplicated genes were specifically expressed tissues examined. qRT-PCR confirmed nine highly...
Abstract Activity of the vascular cambium gives rise to secondary xylem for wood formation in trees. The transcription factor WUSCHEL-related HOMEOBOX4 (WOX4) is a central regulator downstream hormone and peptide signaling pathways that maintain cambial activity. However, genetic regulatory network underlying WOX4-mediated at post-transcriptional level remains be elucidated. In this study, we identified ubiquitin receptor PagDA1 hybrid poplar (Populus alba × Populus glandulosa clone 84K) as...
ABSTRACT Lignin is a major component of plant cell walls and essential for growth development. biosynthesis controlled by hierarchical regulatory network involving multiple transcription factors. In this study, we showed that the gene encoding an APETALA 2/ethylene‐responsive element binding factor (AP2/ERF) factor, PagERF81 , from poplar 84 K ( Populus alba × P. glandulosa ) highly expressed in expanding secondary xylem cells. Two independent homozygous Pagerf81 mutant lines created...
Abstract Wood is formed by the successive addition of secondary xylem, which consists cells with a conspicuously thickened wall composed mainly cellulose, xylan and lignin. Currently, few transcription factors involved in direct regulation biosynthesis have been characterized tree species. Here, we show that PdMYB221, poplar ortholog Arabidopsis R2R3-MYB factor AtMYB4, directly regulates during wood formation. PdMYB221 predominantly expressed developing protein it encodes localizes to...
Pectin, which is a major component of the plant primary cell walls, synthesized and methyl-esterified in Golgi apparatus then demethylesterified by pectin methylesterases (PMEs) located wall. The degree methylesterification affects functional properties pectin, thereby influences growth, development defense. However, little known about mechanisms that regulate demethylesterification. Here, we show Arabidopsis (Arabidopsis thaliana) seed coat mucilage, absence MYB52 transcription factor...
Wood formation involves sequential developmental events requiring the coordination of multiple hormones. Brassinosteroids (BRs) play a key role in wood development, but little is known about cellular and molecular processes that underlie tree species. Here, we generated transgenic poplar lines with edited PdBRI1 genes, which are orthologs Arabidopsis vascular-enriched BR receptors, showed how inhibition signaling influences development at mRNA and/or proteome level. Six Populus genes formed...
Wood (secondary xylem) formation in tree species is dependent on auxin-mediated vascular cambium activity stems. However, the complex regulatory networks underlying xylem remain elusive. Xylem development Populus was characterized based microscopic observations of stem sections transgenic plants. Transcriptomic, quantitative real-time PCR, chromatin immunoprecipitation and electrophoretic mobility shift assay analyses were conducted to identify target genes involved development. Yeast...
Plant CCCH proteins participate in the control of multiple developmental and adaptive processes, but regulatory mechanisms underlying these processes are not well known. In this study, we showed that Arabidopsis (Arabidopsis thaliana) protein C3H15 negatively regulates cell elongation by inhibiting brassinosteroid (BR) signaling. Genetic biochemical evidence functions downstream receptor BR INSENSITIVE 1 (BRI1) as a negative regulator pathway. is phosphorylated GLYCOGEN SYNTHASE KINASE 3...
Necrotrophic pathogens such as Botrytis cinerea cause significant crop yield losses. Plant CCCH proteins play important roles in pathogen resistance responses. However, the CCCH-mediated defense mechanisms against necrotrophic are unclear. Here, we report that Arabidopsis protein C3H14 positively regulates basal B. mainly by WRKY33 signaling. Simultaneous mutation of and its paralog C3H15 resulted enhanced susceptibility to cinerea, while or overexpression lines exhibited reduced...
Plant CCCH zinc finger proteins control growth, development, and stress responses mainly at the post-transcriptional level. Currently, limited reports are available about roles of plant in drought tolerance. In this study, we provided evidence showing that PdC3H17 from Populus deltoides × P. euramericana involves tolerance response. Overexpression poplar caused dwarf, resulted higher stem water potential, showed increased photosynthetic ROS-scavenging abilities, thereby enhancing to stress,...
Abstract Perception of pathogen-associated molecular patterns (PAMPs) triggers mitogen-activated protein (MAP) kinase 4 (MPK4)-mediated phosphorylation and induces downstream transcriptional reprogramming, but the mechanisms MPK4 defense pathway are poorly understood. Here, we showed that phosphorylation-mediated inactivation CCCH C3H14 by positively regulates immune response in Arabidopsis (Arabidopsis thaliana). Compared with wild-type plants, loss-of-function mutations its paralog C3H15...
SUMMARY Heat stress is an environmental factor that significantly threatens crop production worldwide. Nevertheless, the molecular mechanisms governing plant responses to heat are not fully understood. Plant zinc finger CCCH proteins have roles in as well growth and development through protein–RNA, protein–DNA, protein–protein interactions. Here, we reveal integrated multi‐level regulation of thermotolerance mediated by protein C3H15 Arabidopsis. rapidly suppressed transcription, which...