A5060049168- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- RNA Interference and Gene Delivery
- Legume Nitrogen Fixing Symbiosis
- Extracellular vesicles in disease
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Plant Gene Expression Analysis
- Autophagy in Disease and Therapy
- Insect Resistance and Genetics
- Ubiquitin and proteasome pathways
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- RNA modifications and cancer
State Key Laboratory of Plant Genomics
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2016-2025
Institute of Microbiology
2023-2024
Shenzhen University
2023
Hebei Medical University
2021-2023
University of Ottawa
2014-2022
North China University of Water Resources and Electric Power
2022
uOttawa Brain and Mind Research Institute
2022
Sun Yat-sen Memorial Hospital
2015-2016
Although several plant microRNAs (miRNAs) have been shown to play a role in development, no phenotype has yet associated with reduction or loss of expression any miRNA. Arabidopsis thaliana miR164 was predicted target five NAM/ATAF/CUC (NAC) domain-encoding mRNAs, including NAC1, which transduces auxin signals for lateral root emergence. Here, we show that guides the cleavage endogenous and transgenic NAC1 mRNA, producing 3'-specific fragments. Cleavage blocked by mutations disrupt base...
Ubiquitination plays important roles in plant hormone signal transduction. We show that the RING finger E3 ligase, Arabidopsis thaliana SALT- AND DROUGHT-INDUCED FINGER1 (SDIR1), is involved abscisic acid (ABA)-related stress SDIR1 expressed all tissues of and upregulated by drought salt stress, but not ABA. Plants expressing ProSDIR1-beta-glucuronidase (GUS) reporter construct confirmed strong induction GUS expression stomatal guard cells leaf mesophyll under stress. The green fluorescent...
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions play important roles in this pathway. However, due technical limitations only a few instances of ligase-substrate binding protein plants have directly evidenced. An efficient vivo vitro assay was developed for analysis reactions by agroinfiltration expression both substrates ligases Nicotiana benthamiana. Using detailed the...
Nine conserved miRNA families and three potential novel miRNAs in Brassica rapa were identified from a small RNA library. The expression patterns of some had different tissue specificity Arabidopsis. One the miRNAs, named bra‐miR1885, was verified as true functional miRNA. It could be induced specifically by Turnip mosaic virus (TuMV) infection, target TIR–NBS–LRR class disease‐resistant transcripts for cleavage. Based on hypothesis de novo generation new genes sequence similarity between...
Plant viruses are excellent tools for studying microbial-plant interactions as well the complexities of host activities. Our study focuses on role C2 encoded by Beet severe curly top virus (BSCTV) in virus-plant interaction. Using BSCTV bait a yeast two-hybrid screen, C2-interacting protein, S-adenosyl-methionine decarboxylase 1 (SAMDC1), was identified from an Arabidopsis thaliana cDNA library. The interaction confirmed vitro pull-down assay and firefly luciferase complemention imaging...
To reduce the losses caused by plant pathogens, biologists have adopted numerous methods to engineer resistant plants. Among them, RNA silencing-based resistance has been a powerful tool that used crops during last two decades. Based on this mechanism, diverse approaches were developed. In review, we focus application of silencing produce plants are viruses such as and DNA viruses, viroids, insects, recent expansion fungal pathogens.
Summary Constitutive expression of an intron‐containing self‐complementary ‘hairpin’ RNA (ihpRNA) has recently been shown to efficiently silence target genes in transgenic plants. However, this technique cannot be applied whose silencing may block plant regeneration or result embryo lethality. To obviate these potential problems, we have used a chemical‐inducible Cre/ loxP (CLX) recombination system trigger the inverted‐repeat (RNAi) A detailed characterization inducible RNAi Arabidopsis...
Unique among the known plant and animal viral suppressors of RNA silencing, 2b protein interacts directly with both small interfering (siRNA) ARGONAUTE1 (AGO1) AGO4 proteins is targeted to nucleolus. However, it largely unknown which regions 111-residue determine these biochemical properties how they contribute its diverse silencing suppressor activities. Here, we identified a functional nucleolar localization signal encoded within 61-amino acid N-terminal double-stranded (dsRNA) binding...
Short-hairpin RNAs based on microRNA (miRNA) precursors to express the artificial miRNAs (amiRNAs) can specifically induce gene silencing and confer virus resistance in plants. The efficacy of RNA depends not only nature amiRNAs but also local structures target mRNAs. However, lack tools accurately reliably predict secondary within long makes it very hard a viral genome natural infection conditions vivo. In this study, we used an experimental approach dissect how endogenous machinery acts 3'...
The vascular pathogen Verticillium dahliae infects the roots of plants to cause wilt. molecular mechanisms underlying V. virulence and host resistance remain elusive. Here, we demonstrate that a secretory protein, VdSCP41, functions as an intracellular effector promotes virulence. Arabidopsis master immune regulators CBP60g SARD1 cotton GhCBP60b are targeted by VdSCP41. VdSCP41 binds C-terminal portion inhibit its transcription factor activity. Further analyses reveal activation domain...
Verticillium dahliae Kleb. is a hemibiotrophic, phytopathogenic fungus that causes wilt disease in wide range of crops, including cotton. Successful host colonization by hemibiotrophic pathogens requires the induction plant cell death to provide saprophytic nutrition for transition from biotrophic necrotrophic stage. In this study, we identified necrosis-inducing Phytophthora protein (NPP1) domain-containing family containing nine genes virulent, defoliating isolate V. (V592), named VdNLP...
Verticillium dahliae is a phytopathogenic fungus obligate in root infection. A few hyphopodia differentiate from large numbers of hyphae after conidia germination on the surface for further However, molecular features and role pathogenicity V. remain elusive. In this study, we found that VdPls1, tetraspanin, VdNoxB, catalytic subunit membrane-bound NADPH oxidases reactive oxygen species (ROS) production, were specifically expressed hyphopodia. VdPls1 VdNoxB highly co-localize with plasma...
Fungal pathogens secrete effector proteins to suppress plant basal defense for successful colonization. Resistant plants, however, can recognize effectors by cognate R induce effector-triggered immunity (ETI). By analyzing secretomes of the vascular fungal pathogen Verticillium dahliae, we identified a novel secreted protein VdSCP7 that targets nucleus. The green fluorescent (GFP)-tagged gene with either mutated nuclear localization signal motif or additional export was transiently expressed...