A5002641588- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Corneal Surgery and Treatments
- DNA Repair Mechanisms
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Ocular Surface and Contact Lens
- Genetics and Plant Breeding
- Molecular Biology Techniques and Applications
- Plant responses to water stress
- Chromosomal and Genetic Variations
- Ocular Infections and Treatments
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Ocular Disorders and Treatments
China Agricultural University
2015-2025
Ningxia Normal University
2024
Yangtze University
2019-2024
Lanzhou University
2020
Wuhan University
2014
Renmin Hospital of Wuhan University
2014
Abstract Clade A protein phosphatase 2Cs (PP2Cs) are abscisic acid (ABA) co-receptors that block ABA signalling by inhibiting the downstream kinases. is activated after PP2Cs inhibited ABA-bound PYR/PYL/RCAR receptors (PYLs) in Arabidopsis . However, whether these regulated other factors remains unknown. Here, we report ABI1 (ABA-INSENSITIVE 1) can interact with U-box E3 ligases PUB12 and PUB13, but ubiquitinated only when it interacts an vitro assay. mutant form of ABI1-1 unable to PYLs...
A better understanding of the extent convergent selection among crops could greatly improve breeding programs. We found that quantitative trait locus KRN2 in maize and its rice ortholog, OsKRN2, experienced selection. These orthologs encode WD40 proteins interact with a gene unknown function, DUF1644, to negatively regulate grain number both crops. Knockout or OsKRN2 increased yield by ~10% ~8%, respectively, no apparent trade-offs other agronomic traits. Furthermore, genome-wide scans...
Abstract Maize ( Zea mays L.) is a cold-sensitive species that often faces chilling stress, which adversely affects growth and reproduction. However, the genetic basis of low-temperature adaptation in maize remains unclear. Here, we demonstrate natural variation type-A Response Regulator 1 ZmRR1 ) gene leads to differences tolerance among inbred lines. Association analysis reveals InDel-35 , encoding protein harboring mitogen-activated kinase (MPK) phosphorylation residue, strongly...
Phytochromes are red (R) and far-red (FR) light photoreceptors in plants, PHYTOCHROME-INTERACTING FACTORS (PIFs) a group of basic helix-loop-helix family transcription factors that play central roles repressing photomorphogenesis. Here, we report MYB30, an R2R3-MYB factor, acts as negative regulator photomorphogenesis Arabidopsis (Arabidopsis thaliana). We show MYB30 preferentially interacts with the Pfr (active) forms phytochrome A (phyA) B (phyB) holoproteins levels induced by phyA phyB...
The farmland of the world's main corn-producing area is increasingly affected by salt stress. Therefore, breeding salt-tolerant cultivars necessary for long-term sustainability global corn production. Previous studies have shown that natural maize varieties display a large diversity tolerance, yet genetic variants underlying such remain poorly discovered and applied, especially those mediating tolerance to salt-induced osmotic stress (SIOS). Here we report metabolomics-driven understanding...
Verticillium wilt is a severe plant disease that causes massive losses in multiple crops. Increasing the resistance to critical challenge worldwide. Here, we report hemibiotrophic dahliae-secreted Asp f2-like protein VDAL leaf wilting when applied cotton leaves vitro but enhances V. dahliae overexpressed Arabidopsis or without affecting growth and development. interacts with E3 ligases U-box 25 (PUB25) PUB26 ubiquitinated by PUBs vitro. However, not degraded PUB25 planta. Besides, pub25...
DNA and histone methylation coregulate heterochromatin formation gene silencing in animals plants. To identify factors involved maintaining silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II transgenic Arabidopsis (Arabidopsis thaliana) line L119 We identified MAT4/SAMS3/MTO3/AT3G17390, which encodes methionine (Met) adenosyltransferase 4 (MAT4)/S-adenosyl-Met synthetase 3 catalyzes synthesis of...
ABSTRACT Calcium oscillations are induced by different stresses. Calcium‐dependent protein kinases (CDPKs/CPKs) one major group of the plant calcium decoders that involved in various processes including drought response. Some CPKs calcium‐independent. Here, we identified ZmCPK2 as a negative regulator resistance screening an overexpression transgenic maize pool. We found does not bind calcium, and its activity is mainly inhibited during short term abscisic acid (ABA) treatment, dynamically...
Epigenetic markers, such as histone acetylation and DNA methylation, determine chromatin organization. In eukaryotic cells, metabolites from organelles or the cytosol affect epigenetic modifications. However, relationships between modifications are not well understood in plants. We found that peroxisomal acyl-CoA oxidase 4 (ACX4), an enzyme fatty acid β-oxidation pathway, is required for suppressing silencing of some endogenous loci, Pro35S:NPTII ProRD29A:LUC/C24 transgenic line. The acx4...
Stomata, which are essential for the exchange of CO 2 and water vapor between plant leaves atmosphere, regulated by a variety environmental internal factors. In this study, we identified characterized two genes, Guard Cell Turgor Maintaining 1 ( GCT1 ) its closest homolog GCT2 , encode rapidly accelerated fibrosarcoma (RAF)-like protein kinases that play critical role in maintaining guard cell turgor Zea mays . We found overexpression ZmGCT1 ZmGCT2 confers resistance to abscisic acid...
ABSTRACT Drought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression drought‐responsive genes is critical for understanding transcriptional regulatory networks in response to drought, which facilitates improvement drought tolerance. Here, we identified an Alfin‐like (AL) family gene ZmAL14 negatively regulates resistance. Overexpression exhibits susceptibility while mutation enhances An abscisic acid...
Significance The plant phytochrome molecule consists of an N-terminal photosensory domain and a C-terminal dimerization domain, connected by flexible hinge region. It was previously shown that serine residue in the region oat A (phyA) could be phosphorylated vivo plays important role regulating phyA function. However, little is known regarding function Arabidopsis phyA. Here, we show three residues are essential phosphorylation Our study thus provides new insights into regulatory region,...
DNA methylation and histone modification are important epigenetic marks that coregulate gene expression genome stability. To identify factors involved in chromatin silencing, we carried out a forward genetic screen for mutants release the silenced Pro-35S:LUCIFERASE (35SP-LUC) Arabidopsis (Arabidopsis thaliana). We identified an regulator, METHIONINE SYNTHASE1 (ATMS1), which catalyzes synthesis of methionine (Met) one-carbon metabolism pathway. The ATMS1 mutation releases 35SP-LUC majority...
Phytochrome A (phyA) is the far-red (FR) light photoreceptor in plants that essential for seedling de-etiolation under FR-rich environments, such as canopy shade. TANDEM ZINC-FINGER/PLUS3 (TZP) was recently identified a key component of phyA signal transduction Arabidopsis thaliana; however, how TZP integrated into signaling networks remains largely obscure. Here, we demonstrate ELONGATED HYPOCOTYL5 (HY5), well-characterized transcription factor promoting photomorphogenesis, mediates FR...
DNA polymerase δ plays crucial roles in repair and replication as well maintaining genomic stability. However, the function of POLD2, second small subunit δ, has not been characterized yet Arabidopsis (Arabidopsis thaliana). During a genetic screen for release transcriptional gene silencing, we identified mutation POLD2. Whole-genome bisulfite sequencing indicated that POLD2 is involved regulation methylation. genetically interacts with Ataxia Telangiectasia-mutated Rad3-related α The...
Minichromosome Maintenance protein 10 (MCM10) is essential for DNA replication initiation and elongation in yeasts animals. Although the functions of MCM10 repair have been well documented, detailed mechanisms these processes are not known. Here, we identified AtMCM10 gene through a forward genetic screening releasing silenced marker gene. plant possesses similar crystal structure as animal MCM10, growth or development Arabidopsis. can directly bind to histone H3-H4 promotes nucleosome...
Potassium (K + ) is one of essential mineral elements for plant growth and development. K channels, especially AKT1-like play crucial roles in uptake roots. Maize important crops; however, the mechanism maize little known. Here, we report physiological functions channel ZMK1 homeostasis maize. a homolog Arabidopsis AKT1 maize, mainly expressed root. Yeast complementation experiments electrophysiological characterization Xenopus oocytes indicated that could mediate uptake. rescued low-K...
Repression of embryonic traits during the seed-to-seedling phase transition requires inactivation master transcription factors associated with embryogenesis. How timing such is controlled unclear. Here, we report on a novel transcriptional co-repressor, Arabidopsis thaliana SDR4L, that forms feedback inhibition loop LEC1 and ABI3 to repress post-imbibition. regulate their own expression by inducing AtSDR4L mid late binds sites upstream ABI4, these transcripts are upregulated in Atsdr4l...
ABSTRACT Calcium oscillations are induced by different stresses. Calcium-dependent protein kinases (CDPKs/CPKs) one major group of the plant calcium decoders that involved in various processes including drought response. Some CPKs calcium-independent. Here, we identified ZmCPK2 as a negative regulator resistance screening an overexpression transgenic maize pool. We found does not bind calcium, and its activity is modulated likely oscillation manner during short time ABA treatment....
Summary Drought severely affects crop growth and yields. Stomatal regulation plays an important role in plant response to drought stress. Light‐activated plasma membrane‐localized proton ATPase (PM H + ‐ATPase) mainly promoted the stomatal opening. Abscisic acid (ABA) a dominant closure during It is not clear how PM ‐ATPase involved of ABA‐induced closure. We found that CALCIUM‐DEPENDENT PROTEIN KINASE RELATED 1 (ZmCRK1), its mutant zmcrk1 exhibited slow water loss detached leaves,...
It is vital to develop high-throughput methods determine transgene copy numbers initially and zygosity during subsequent breeding. In this study, the target sequence of previously reported endogenous reference gene hmg was analyzed using 633 maize inbred lines, two SNPs were observed. These significantly increased PCR efficiency, while newly developed assay (hmg-taq-F2/R2) excluding these reduced efficiency into normal ranges. The TaqMan amplification bar with primers calculated as 0.993...