A5077015896- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Light effects on plants
- CAR-T cell therapy research
- Photosynthetic Processes and Mechanisms
- Virus-based gene therapy research
- RNA Research and Splicing
- Plant nutrient uptake and metabolism
- Melanoma and MAPK Pathways
- Liver Disease Diagnosis and Treatment
- Pharmacogenetics and Drug Metabolism
- GABA and Rice Research
- Monoclonal and Polyclonal Antibodies Research
- Plant responses to elevated CO2
- Lipid metabolism and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Viral Infections and Immunology Research
- Dermatology and Skin Diseases
- Seed Germination and Physiology
- RNA modifications and cancer
- Salivary Gland Disorders and Functions
- Plant Gene Expression Analysis
- Plant responses to water stress
- Computational Drug Discovery Methods
- Drug Transport and Resistance Mechanisms
China Agricultural University
2021-2025
Peking Union Medical College Hospital
2022-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands cold-responsive (COR) genes. Essential to this process is C-repeat binding factor (CBF)-dependent pathway, involving activity AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) determine...
Abstract Maize ( Zea mays L.) is a cold-sensitive species that often faces chilling stress, which adversely affects growth and reproduction. However, the genetic basis of low-temperature adaptation in maize remains unclear. Here, we demonstrate natural variation type-A Response Regulator 1 ZmRR1 ) gene leads to differences tolerance among inbred lines. Association analysis reveals InDel-35 , encoding protein harboring mitogen-activated kinase (MPK) phosphorylation residue, strongly...
Maize (Zea mays) originated in tropical areas and is thus susceptible to low temperatures, which pose a major threat maize production. Our understanding of the molecular basis cold tolerance limited. Here, we identified bZIP68, basic leucine zipper (bZIP) transcription factor, as negative regulator maize. Transcriptome analysis revealed that bZIP68 represses cold-induced expression DREB1 factor genes. The stability transcriptional activity are controlled by its phosphorylation at conserved...
Exposure to cold triggers a spike in cytosolic calcium (Ca 2+ ) that often leads transcriptional reprogramming plants. However, how this Ca signal is perceived and relayed the downstream signaling pathway remains unknown. Here, we show CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) initiates phosphorylation cascade specify of cold-induced signal. Plasma membrane (PM)–localized CPK28 activated rapidly upon shock within 10 seconds -dependent manner. then phosphorylates promotes nuclear...
Abstract Plants respond to cold stress at multiple levels, including increasing cytosolic calcium (Ca2+) influx and triggering the expression of cold-responsive genes. In this study, we show that Ca2+-permeable channel CYCLIC NUCLEOTIDE–GATED CHANNEL20 (CNGC20) positively regulates freezing tolerance in Arabidopsis (Arabidopsis thaliana) by mediating cold-induced Ca2+ influx. Moreover, demonstrate leucine-rich repeat receptor-like kinase PLANT PEPTIDE CONTAINING SULFATED TYROSINE1 RECEPTOR...
Abstract Light and temperature are two key environmental factors that coordinately regulate plant growth development. Although the mechanisms integrate signaling mediated by cold red light have been unraveled, roles of blue photoreceptors cryptochromes in responses to remain unclear. In this study, we demonstrate CRYPTOCHROME2 (CRY2)-COP1-HY5-BBX7/8 module regulates light-dependent acclimation Arabidopsis thaliana. We show phosphorylated forms CRY2 induced stabilized stress cold-stabilized...
ABSTRACT Deoxycholic acid (DCA) injection is applied in treating moderate to severe submental bulge or facial fullness caused by excessive fat accumulation. Using ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) technology, which was swiftly, precisely, and reliably confirmed, DCA determined human plasma with low quantification limits of 56 ng/mL. We selected six healthy individual blank concentrations endogenous mixed them prepare standard curve samples. The...
Deoxycholic acid (DCA) injection is applied in treating moderate to severe submental bulge or facial fullness caused by excessive fat accumulation. Utilizing the method known as ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which was swiftly, precisely, and reliably confirmed, DCA determined human plasma with low quantification limits of 56 ng/mL. We selected six healthy individual blank concentrations endogenous deoxycholic mixed them prepare standard curve...
Summary Cold stress is a major abiotic that threatens maize ( Zea mays L.) production worldwide. Understanding the molecular mechanisms underlying cold tolerance crucial for breeding resilient varieties. Tonoplast intrinsic proteins (TIPs) are subfamily of aquaporins in plants. Here, we report TIP family involved tolerance. The expression most genes was responsive to stress. Overexpressing TIP2;1 , TIP3;2 or TIP4;3 reduced seedlings, while loss‐of‐function mutants exhibited enhanced...
Background: Malignant melanoma is an aggressive disease. Tunlametinib (HL-085) a potent, selective, and orally bioavailable MEK1/2 inhibitor. The objective of this study was to determine the pharmacokinetics (PK) tunlametinib its main metabolite M8 in patients with NRAS-mutant following single dose multiple doses phase I safety PK study. Methods: A multiple-center performed including dose-escalation dose-expansion phase. oral 0.5-18 mg twice daily assessed. Results: total 30 participants...
Anti-AAV neutralizing Abs (NAbs) titer is usually measured by cell-based microneutralization (MN) assay and crucial for patient screening in AAV-based gene therapy clinical trials. However, achieving uniform operation comparable results among different laboratories remains challenging. Here, we established a standardized MN anti-AAV9 NAbs human sera or plasma transferred the method to other two research teams. Then, validated its parameters tested set of eight samples blind across all...