A5101441608- Congenital Heart Disease Studies
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- COVID-19 Clinical Research Studies
- Cardiac Structural Anomalies and Repair
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Metabolism and Genetic Disorders
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Hepatitis C virus research
- Cardiac Arrhythmias and Treatments
- Oral and Maxillofacial Pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Ultrasound in Clinical Applications
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Vascular anomalies and interventions
- Cardiovascular Issues in Pregnancy
- Virus-based gene therapy research
First Affiliated Hospital of Zhengzhou University
2012-2022
Renmin Hospital of Wuhan University
2013-2021
Wuhan University
2013-2021
Jinan Military General Hospital
2014-2018
Children's Hospital of Fudan University
2017
Wenzhou Medical University
2017
First Affiliated Hospital of Wenzhou Medical University
2017
Sichuan University
2017
Anhui Medical University
2017
Fudan University
2017
Summary Background 2019-Novel coronavirus (2019-nCoV) outbreaks create challenges for hospital laboratories because thousands of samples must be evaluated each day. Sample types, interpretation methods, and corresponding laboratory standards established. The possibility other infections should assessed to provide a basis clinical classification, isolation, treatment. Accordingly, in the present study, we testing methods 2019-nCoV co-infections. Methods We used fluorescence-based quantitative...
The modification of chimeric antigen receptor (CAR) endowing T cells with tumor-specific cytotoxicity induces antitumor immunity. However, the structural characteristics solid tumors, loss specific antigens, and strong immunosuppressive environment are challenges to treat tumors CAR-T therapy. purpose our study was find verify potentials therapies for patients head neck squamous cell carcinoma (HNSCC). First, we selected MUC1 as research target verified its differential expression in cancer...
Folate deficiency is an independent risk factor for congenital heart disease (CHD); however, the maternal plasma folate level paradoxically not a good diagnostic marker. Genome-wide surveys have identified variants of nonfolate metabolic genes associated with level, suggesting that these genetic polymorphisms are potential factors CHD.To examine effects concentration-related variations on CHD in Han Chinese population, we performed 3 case-control studies including total 1489 patients and...
The aims of the present study are to investigate sub-cellular location, differential expression in different cancer stages and functional role FAM134B colon development. was studied quantified at protein mRNA levels cell lines using immunocytochemistry, Western blot real-time PCR. In vitro assays an vivo xenotransplantation mouse models were used molecular biology response silencing with shRNA lentiviral particles. noted both cytoplasm nuclei cells. cells derived from stage IV cancer,...
This study aimed to elucidate how microRNA27a-3p (miR-27a-3p) modulates the Wnt/β-catenin signaling pathway promote epithelial-mesenchymal transition (EMT) in oral squamous carcinoma stem cells (OSCSCs) by targeting secreted frizzled-related protein 1 (SFRP1). Flow cytometry was used sort OSCSCs from SCC-9 and Tca8113 cell lines. The were randomly assigned into miR-27a-3p inhibitors group, inhibitors-NC si-SFRP1 + group blank group. A luciferase reporter, immunofluorescence Transwell assays...
Tongue squamous cell carcinoma (TSCC) is highly malignant and poorly differentiated, resulting in a high frequency of local recurrence distant metastases. Sox2 (Sry‑box2), an important factor embryonic development differentiation, has been shown to associate with phenotypes epithelial‑mesenchymal transition (EMT) progression numerous types human tumors. However, the clinical relevance molecular mechanisms TSCC remain unclear. In present study, expression levels were assessed 61 pairs samples...
Abstract Background The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever dry cough. Methods 1008 severe the mixed specimen of nasopharyngeal swab and oropharyngeal by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor 12 types specimens RT-qPCR during hospitalization. All 20 cases detect isolation period 7 before releasing period. Results Of enrolled showed highest...
Abstract Background Lymphocyte count ( LYM) of peripheral blood and some indices general biochemical analysis had diagnostic prognostic value for coronavirus disease 2019 (COVID‐19), the other remaining is rare. Methods A total 94 patients with COVID‐19 were enrolled at Renmin Hospital Wuhan University. According to severity COVID‐19, divided into three groups (moderate 49, severe 35, critical 10), 40 healthy cases in same period as controls. The cell was analyzed. Results Compared cases,...
The identification of novel biomarkers cancer is important for improved diagnosis and prognosis. With an abundant amount resources in the publicly available database, such as Cancer Genome Atlas (TCGA) integrative strategy used to systematically characterize aberrant patterns colorectal (CRC) based on RNA-Seq, chromatin immunoprecipitation sequencing (ChIP-Seq), tissue microarray (TMA), gene profiling molecular signatures. expression transcription factor ATF3 was elevated human CRC specimens...
Abstract TBX5 is a vital transcription factor involved in cardiac development dosage-dependent manner. But little known about the potential association of 3′ untranslated region (UTR) variations with congenital malformations. This study aimed to investigate relationship between 3′UTR variants and risk for heart disease (CHD) susceptibility two Han Chinese populations, reveal its molecular mechanism. The CHD was examined 1 177 patients 990 healthy controls independent case–control studies....
In recent years, researchers have found the critical role of telomerase in cellular transformation, proliferation, stemness and cell survival. High levels reverse transcriptase (TERT) expression activation been reported most cancer cells. Moreover, overexpression human TERT (hTERT) is to be correlated with advanced invasive stage tumor progression poor prognosis. Epithelial-mesenchymal transition (EMT), characterized by loss cell-cell contact epithelial cells acquisition migratory motile...
Evidence has shown that mesenchymal-epithelial transition (MET) and epithelial-mesenchymal (EMT) are linked to stem cell properties. We currently lack a model showing how the occurrence of MET EMT in immortalised cells influences maintenance Thus, we established project aiming investigate roles acquisition properties oral epithelial cells.In this study, retroviral transfection vector (pLXSN-hTERT) was used immortalise by insertion hTERT gene (hTERT(+)-oral mucosal line [OME]). The protein...
Abstract Adenoid cystic carcinoma (ACC) is characterized by intensive local invasion and high incidence of distant metastases. Conventional chemotherapy for ACC produces a poor result. We aimed to evaluate the effect apple polyphenols (APs), novel nutraceutical agent, on proliferation apoptosis levels in metastatic oral cell line. A (ACC‐M) line control cells (MRC‐5 derived from normal lung tissue) were treated with APs at different concentrations. MTT assay was used determine vitro...
// Bin Qiao 1, * , Baoxia He 2, Jinghua Cai 1 Alfred King-Yin Lam 3 and Wei Department of Stomatology, The First Affiliated Hospital Zhengzhou University, Zhengzhou, Henan, P.R. China 2 Pharmacy, Cancer Henan Hospital, Molecular Pathology, School Medicine Menzies Health Institute Queensland, Griffith Gold Coast, Australia These authors contributed equally to this work Correspondence to: Qiao, email: qiaobin@zzu.edu.cn He, hewei1968@tom.com Keywords: oral carcinoma; selenium; oxidative...
The aim is to investigate the impacts of using multiplex immunochemistry (mIHC) staining analyses co-expression programmed death ligand-1 (PD-L1) and tumor infiltrating lymphocytes (TILs) [CD8+ T cells Forkhead Box Protein 3 (FOXP3)+ regulatory (Tregs)] in different oral diseases, squamous cell carcinoma (OSCC).Formalin fixed paraffin-embedded tissue sections from diseases were stained with PD-L1 TILs (CD8+ FOXP3+ Tregs) by mIHC simultaneously. whole slide was scanned digitally observe...
Oct4 and Sox2 are pluripotent stem cell factors but the interplay between them in tumorigenesis is unclear. The aim of present study was to investigate roles reprogramming oral cancer cells. One or both were overexpressed immortalized epithelial (hTERT+-OME) cells by lentivirus transduction. In addition, proteins two squamous carcinoma (OSCC) lines (Cal27 primary cultured OSCC from a T2N2M0 patient) individually combinedly knocked down shRNA. results showed that doubly transduced...
This study is to explore the clinical significance of folate receptor-positive circulating tumor cells (FR+ CTC) in early diagnosis and disease progress patients with breast cancer.Folate was enriched from peripheral blood immunomagnetic separation method quantitated by receptor on CTC ligand-targeted PCR.The levels FR+ were significantly higher cancer compared healthy controls. Detective rate decreased 19 27 underwent surgery 2 weeks post-operation pre-operation; statistical analysis showed...