A5102823042- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- COVID-19 diagnosis using AI
- Advanced biosensing and bioanalysis techniques
- Hepatitis B Virus Studies
- Tumors and Oncological Cases
- Nanopore and Nanochannel Transport Studies
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Cancer and Skin Lesions
- Traditional Chinese Medicine Studies
- Eicosanoids and Hypertension Pharmacology
- Estrogen and related hormone effects
- Pancreatic and Hepatic Oncology Research
- Orthopaedic implants and arthroplasty
- Colorectal Cancer Screening and Detection
- Dental Implant Techniques and Outcomes
- Inflammatory mediators and NSAID effects
- Hepatitis C virus research
- Bone Tissue Engineering Materials
- Hormonal and reproductive studies
- Acute Myeloid Leukemia Research
Wuhan University
2012-2024
Renmin Hospital of Wuhan University
2012-2024
Zhejiang Taizhou Hospital
2009
Wenzhou Medical University
2009
Central South University
2008
The ongoing global novel coronavirus pneumonia COVID-19 outbreak has engendered numerous cases of infection and death. diagnosis relies upon nucleic acid detection; however, currently recommended methods exhibit high false-negative rates are unable to identify other respiratory virus infections, thereby resulting in patient misdiagnosis impeding epidemic containment. Combining the advantages targeted amplification long-read, real-time nanopore sequencing, herein, sequencing (NTS) is...
Abstract The ongoing novel coronavirus pneumonia COVID-19 outbreak in Wuhan, China, has engendered numerous cases of infection and death. diagnosis relies upon nucleic acid detection; however, current recommended methods exhibit high false-negative rates, low sensitivity, cannot identify other respiratory virus infections, thereby resulting patient misdiagnosis impeding epidemic containment. Combining the advantages target amplification long-read, real-time nanopore sequencing, we developed...
Small 2020, 16, 2002169 DOI: 10.1002/smll.202002169 In the originally published article, Data Availability paragraph of experimental section was mistakenly deleted during copyediting process. The original sequencing dataset has been submitted to China National Center for Bioinformation GSA database (http://ngdc.cncb.ac.cn/gsa/), with accession number CRA004499. This update can link research paper. editorial office apologizes any inconvenience caused.
Abstract Background The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever dry cough. Methods 1008 severe the mixed specimen of nasopharyngeal swab and oropharyngeal by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor 12 types specimens RT-qPCR during hospitalization. All 20 cases detect isolation period 7 before releasing period. Results Of enrolled showed highest...
Abstract Background Colorectal cancer (CRC) ranks as the third most common malignancies in world, and periodic examination of patient is advantageous reducing mortality CRC. The first blood-based Septin9 gene methylation assay which recognized by US FDA for CRC was Epi proColon. However, this not broadly applied current clinical guideline because its relatively lower sensitivity detection early-stage Methods This study aimed at developing a new multiplex (ColonUSK) simultaneously evaluates...
HBV (hepatitis B virus) genotyping is important in determining the clinical manifestation of disease and treatment response, particularly, patients with low viral loads. Also, sensitive detection antiviral drug resistance mutations essential for monitoring therapy response. Asensitive direct sequencing method mutation levels DNA patients' plasma developed by PCR amplification novel universal primers. The novel, common, primers were identified alignment RT region all sequences databases....
Estrogens protect the vascular system in women, but its effect men is unclear. We evaluated impact of estrogen on male cardiovascular system. Of 140 Chinese males, 55 (aged 61.2 ± 3.5) were cases and 60 59.5 4.6) controls. Compared with control group, only serum estradiol ([E2]; P < .01) levels not testosterone ([T]; = .21) significantly lower cases. Linear multiple regression analysis showed that T was positively associated triglycerides ([TG]; r .439, d-dimer (r .258, .05) negatively...
Objective To investigate the positive rate for 2019-nCoV tests and co-infections in Wuhan district. Methods A total of 8 274 cases were enrolled this cross-sectional study during January 20 to February 9, 2020, tested using fluorescence quantitative PCR. Both respiratory tract samples (nasopharynx, oropharynx, sputum alveolar lavage fluid) non-respiratory (urine, feces, anal swabs, blood conjunctival sac swabs) collected. If both orf1ab N genes are positive, they classified as nucleic...
We aimed to analyze clinical characteristics and find potential factors predict poor prognosis in patients with coronavirus disease 2019 (COVID-19).
In December, the outbreak of a novel coronavirus (2019-nCoV) in Wuhan, China, has attracted extensive global attention. On January 20, 2020,the Chinese health authorities upgraded to Class B infectious disease Law People's Republic China on Prevention and Treatment Infectious Diseases, considered it as A diseases control prevention. 22, 2020, 2019-nCoV nucleic acid detection test was listed diagnostic criteria guidelines for diagnosis treatment pneumonia due (Trial Version 2) . Therefore,...
The outbreak and widely spread of coronavirus disease 2019 (COVID-19) has become a global public health concern. COVID-19 caused an unprecedented profound impact on the whole world, prevention control is challenge remains to be solved. retrospective analysis large scale tests SARS-CoV-2 RNA may indicate some important information this pandemic. We selected 12400 detected in Wuhan first semester 2020 made systematic them, order find beneficial clue for consistent COVID-19.SARS-CoV-2 was...
In December, the outbreak of a novel coronavirus (2019-nCoV) in Wuhan, China, has attracted extensive global attention. On January 20, 2020, Chinese health authorities upgraded to Class B infectious disease Law People′s Republic China on Prevention and Treatment Infectious Diseases, considered it as A diseases control prevention. 18, 2019-nCoV nucleic acid detection test was listed diagnostic criteria guidelines for diagnosis treatment pneumonia due (Trial Version 2) . Therefore,...
Abstract Background The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever dry cough. Methods 1008 severe the mixed specimen of nasopharyngeal swab and oropharyngeal by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor 12 types specimens RT-qPCR during hospitalization. All 20 cases detect isolation period 7 before releasing period. Results Of enrolled showed highest...
Abstract Background: We aimed to analyse clinical characteristics and find potential factors predicting poor prognosis in patients with coronavirus disease 2019 (COVID-19). Methods: analyzed the demographic data of COVID-19 detected SARS-CoV-2 RNA urine sediments collected from 53 enrolled Renmin Hospital Wuhan University January 31, 2020 February 18, qRT-PCR analysis, then classified those based on conditions (severe or non-severe syndrome) urinary (U - U + ). Results: found that severe...
The routine teaching mode of diabetes mellitus (DM) is divided into various sub-majors medical laboratory, which not conducive to clinical laboratory physicians quickly mastering relevant knowledge. A novel DM testing pathway established improve efficiency and enhance the effects talent cultivation in medicine.The guidelines expert consensuses were gathered from professional websites databases. diagnostic was formulated, questionnaire mutual evaluation used evaluate effectiveness 8-year...
Objective To explore the accuracy and clinical application of Pyrosequencing for detection drug resistant relevant mutation in polymerase gene Hepatitis B virus (HBV). Methods Compared with Sanger sequencing,the sensitivity were assessed.Pyrosequencing was used to determine serum 1164 patients chronic its results analyzed. Results The 1×103KIU/L,the same as sequencing.But more stable specific than sequencing detect low rate over 10% mutation.The mutations couldn′t be detected...
患儿女，8个月。第二胎足月顺产，出生即被发现右颈部肿物约8cm×7cm×6cm，转颈及呼吸未见明显影响，初步诊断为颈部畸胎瘤。心脏超声提示房间隔缺损（缺损直径3mm）当时考虑患儿年龄太小，营养不良，房间隔缺损，手术风险大，未行治疗。
Objective To evaluate and analyze the clinical application value of detection Warfarin-related gene polymorphisms, cytochrome P450 2C9 (CYP2C9) Vitamin K epoxide reductase complex subunit 1 (VKORC1) polymorphisms. Methods From July 2011 to 2012, blood samples were randomly collected from 140 lung cancer patients Department Oncology in Renmin Hospital Wuhan University.These diagnosed through imaging examination pathological examination.CYP2C9 VKORC1 polymorphisms detected 70 (studied...