A5101558726- Immune Response and Inflammation
- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Inflammatory mediators and NSAID effects
- Civil and Geotechnical Engineering Research
- Immune Cell Function and Interaction
- Renal and related cancers
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Systemic Lupus Erythematosus Research
- COVID-19 diagnosis using AI
- High-Voltage Power Transmission Systems
- Cancer Research and Treatments
- Alcohol Consumption and Health Effects
- Psoriasis: Treatment and Pathogenesis
- Acute Kidney Injury Research
- Genetic and Kidney Cyst Diseases
- Optimal Power Flow Distribution
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Structural Engineering and Vibration Analysis
Wuhan University
2014-2025
Renmin Hospital of Wuhan University
2013-2025
Hubei Provincial Center for Disease Control and Prevention
2024
North China Electric Power University
2022
Shanghai Changzheng Hospital
2014
Second Military Medical University
2014
Central South University
2011
GTx (United States)
2008
Hunan Communications Research Institute
2005
Albert Einstein College of Medicine
1999-2000
Sustained activation of autophagy and lipid accumulation in tubular epithelial cells (TECs) are both associated with the kidney fibrosis progression. Autophagy has been found involved metabolism regulation through a bi-directional mechanism inducing lipolysis as well promoting droplet formation. However, whether how influences remain unclear. In current study, we show that UUO-induced was significantly reduced when pharmacological inhibitor 3-MA or CQ administrated vivo vitro. Of interest,...
Renal tubular epithelial cells (TECs) undergo an energy-related metabolic shift from fatty acid oxidation to glycolysis during chronic kidney disease (CKD) progression. However, the mechanisms underlying this burst of remain unclear. Herein, a new critical regulator, transcription factor forkhead box protein K1 (FOXK1) that is expressed in TECs renal fibrosis and exhibits fibrogenic metabolism-rewiring capacities reported. Genetic modification Foxk1 locus alters glycolytic metabolism...
The source of IgA and the mechanism for deposition in mesangium remain unknown primary nephropathy. Because CD19+CD5+ B cells are important producers contribute to several autoimmune diseases, they may play an role In this study, flow cytometry, quantitative PCR, confocal microscopy were used assess frequency, distribution, Ig production, CD phenotypes, cytokine sensitivity apoptosis peripheral blood, peritoneal fluid, kidney biopsies 36 patients with All nephropathy significantly more...
Abstract Both clinical and laboratory reports indicate that ethanol addicts are prone to recurrent infections. We hypothesize promotes macrophage apoptosis, thus compromising the efficiency of mononuclear phagocyte system in dealing with infection. studied effect on apoptosis. Human monocytes isolated from healthy subjects after an alcohol drinking binge showed enhanced apoptosis (before, 1.2 ± 0.3% vs after, 28.4 3.7% apoptotic cells/field). Peritoneal macrophages harvested ethanol-treated...
Abstract Background To investigate the effect of oxidized low density lipoprotein receptor-1 (LOX-1) on tubular epithelial-mesenchymal transition (TEMT) induced by low-density (ox-LDL) and its mechanism. Methods NRK-52E cells were incubated with ox-LDL (0, 25, 50, 100 μg/ml) for 24 hours or pre-treated chemical inhibitor LOX-1 receptor polyinosinic acid (poly I) carrageenan antioxidant N-acetyl-L-cysteine (NAC), then exposed to 50 μg/ml ox-LDL.The expression LOX-I, E-cadherin, α-smooth...
Abstract Renal fibrosis is controlled by profibrotic and antifibrotic forces. Exploring anti-fibrosis factors mechanisms an attractive strategy to prevent organ failure. Here we identified the JNK-associated leucine zipper protein (JLP) as a potential endogenous factor. JLP, predominantly expressed in renal tubular epithelial cells (TECs) normal human or mouse kidneys, was downregulated fibrotic kidneys. Jlp deficiency resulted more severe unilateral ureteral obstruction (UUO) mice, while...
The aim of the present study was to investigate impact three different blood purification methods, hemoperfusion (HP), continuous (CBP), and on-line high-volume hemodiafiltration (OL-HDF), on survival rate patients with acute severe organophosphorus pesticide poisoning (ASOPP), as well major pro-inflammatory (interleukin [IL]-1, IL-6, tumor necrosis factor-α [TNF-α]) anti-inflammatory (IL-10) cytokines in serum. Eighty-one ASOPP were randomly divided into groups: HP (N = 23), + CBP 26),...
CD19+ CD24hi CD38hi cells play an essential role in maintaining immune homeostasis. CD40 signaling is involved regulating the induction and function of cells. Changes B-cell subpopulations have been observed systemic lupus erythematosus (SLE) patients. Whether changes subpopulation are related to aberrant SLE patients remains unclear. In this study, we examined levels CD38low peripheral blood mononuclear serum level soluble ligand (sCD40L) 30 with SLE. Through routine biochemical assays flow...
Glomerular epithelial cells (GEC) have been demonstrated to undergo morphological alterations in human immunodeficiency virus (HIV)-associated focal glomerulosclerosis. In the present study, we evaluated effect of HIV-1 gp120 envelope protein on growth cultured (H) GEC. enhanced (P < 0.001) proliferation HGEC at lower concentrations. The mitogenic was further confirmed by accumulation proliferating nuclear cell antigen (PCNA) protein-treated cells, as compared with control cells. On...
Alport syndrome (AS) is a hereditary nephropathy characterized by glomerular basement membrane lesions. AS shows relatively rare entity with autosomal dominant gene mutation (accounts for less than 5 percent of cases) and widely believed to be consequence heterozygous variants in the COL4A3 COL4A4 genes. Until now, there have been no reports homozygous genes patients, it scarce detect both single pedigree. We performed genetic analysis exome sequencing (exome-seq) Chinese family found four...
<b><i>Background:</i></b> Peritoneal dialysis-related peritoneal fibrosis is the leading cause of ultrafiltration failure. Multitude factors and pathological processes have been implicated in development progression, whereas intrinsic anti-fibrotic mechanism has rarely explored. JNK-associated leucine zipper protein (JLP) recently found possessing powerful merits overall antagonizing TGF-β-induced profibrotic effects. <b><i>Objectives:</i></b>...
Background Surfactant protein A (SP-A) contributes to the regulation of sepsis-induced acute lung injury. In a previous study, we demonstrated expression and localization SP-A in kidneys. The present study evaluated effect on lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) its underlying mechanisms human renal tubular epithelial (HK-2) cells. Methods Indirect immunofluorescence assay was used detect distribution HK-2 cells were treated with various concentrations LPS (0,...
Abstract Background: We aimed to analyse clinical characteristics and find potential factors predicting poor prognosis in patients with coronavirus disease 2019 (COVID-19). Methods: analyzed the demographic data of COVID-19 detected SARS-CoV-2 RNA urine sediments collected from 53 enrolled Renmin Hospital Wuhan University January 31, 2020 February 18, qRT-PCR analysis, then classified those based on conditions (severe or non-severe syndrome) urinary (U - U + ). Results: found that severe...
Objective To investigate the effect of Simvastatin on LOX-1 and ROS in NRK52E induced by ox-LDL.Methods NRK-52E cells were divided into three groups: Control group,ox-LDL group (50 μg/ml ox-LDL) (10 μmol/L +50 ox-LDL).After incubation for 24 h,the expression LOX-I was analyzed Western blotting,and production reactive oxygen species (ROS) with confocal laser scanning microscopy.Results expressed at low level,50 ox-LDL increased 6.80 times.Pre - treatment decreased 65％.There little generation...
Objective To investigate the association between expression of interleukin-17 (IL-17) in renal tubuleinterstitium and diabetic nephropathy.Methods 55 rats were randomly divided into three groups:control group,diabetic rat group.Diabetic group was injected 50 mg/kg streptozoticin (STZ).In 2th,4th week,serum urine IL-17 measured with enzyme-linked immunosorbent assay (ELISA).The IL-17,α-smooth muscle actin (α-SMA) nuclear factor-κB p65 (NF-κB p65) kidney assessed by immunohistochemisty...
Objective To investigate the effect of lectin like oxidized low density lipoprotein receptor 1 ( LOX-1 ) in NRK52E intaking lipid induced by ox-LDL). Methods NRK-52E was incubated with ox-LDL (0,25,50, and 100 g/ml for 24 hours or pre-treated chemical blocker receptor- polyI carrageenan, then exposed to 50 μg/ml ox-LDL. LOX-Ⅰ mRNA examined real-time PGR. protein assessed Western blot analysis. Lipid deposit oil red O. Results expression 25,50,100 group 2. 13, 10. 14, 20. 81 times that 0 P...