A5101664024- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- RNA Research and Splicing
- COVID-19 Impact on Reproduction
- Retinal Diseases and Treatments
- SARS-CoV-2 detection and testing
- Liver Disease Diagnosis and Treatment
- Inflammasome and immune disorders
- Circular RNAs in diseases
- RNA regulation and disease
- COVID-19 and healthcare impacts
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Bioinformatics and Genomic Networks
- Inflammatory Biomarkers in Disease Prognosis
- Ocular Diseases and Behçet’s Syndrome
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Gout, Hyperuricemia, Uric Acid
Central Hospital of Wuhan
2020-2025
General Hospital of Central Theater Command
2020-2024
Wuhan General Hospital of Guangzhou
2010-2023
Southern Medical University
2020-2023
Command Hospital
2020-2023
Army Medical University
2009-2021
Weatherford College
2021
Chinese People's Liberation Army
2019
General Hospital of Guangzhou Military Command
2013-2017
Wuhan University
2013
BACKGROUND The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has posed great threat to human health. T cells play a critical role in antiviral immunity but their numbers and functional state COVID-19 patients remain largely unclear. METHODS We retrospectively reviewed the counts serum cytokine concentration from data 522 with laboratory-confirmed 40 healthy controls. In addition, expression cell exhaustion markers were measured 14...
Abstract It is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can directly infect human kidney, thus leading to kidney injury (AKI). Here, we perform a retrospective analysis of clinical parameters from 85 patients with laboratory-confirmed disease 2019 (COVID-19); moreover, histopathology six additional COVID-19 post-mortem examinations was performed. We find that 27% (23/85) exhibited AKI. The elderly and cases comorbidities (hypertension heart failure) are...
Summary BACKGROUND The outbreak of a novel coronavirus (SARS-CoV-2, previously provisionally named 2019 or 2019-nCoV) since December in Wuhan, China, has become an emergency major international concern. Apart from the respiratory system, it is unclear whether SARS-CoV-2 can also directly infect other tissues such as kidney induce acute renal failure. METHODS We conducted retrospective analysis estimated glomerular filtration rate (eGFR) along with clinical parameters 85 patients...
Summary BACKGROUND Nucleic acid test and antibody assay have been employed in the diagnosis for SARS-CoV-2 infection, but use of viral antigen has not successfully developed. Theoretically, is specific marker virus precedes appearance within infected population. There a clear need detection rapid early diagnosis. METHODS We included cohort 239 participants with suspected infection from 7 centers study. measured nucleocapsid protein nasopharyngeal swab samples parallel nucleic test. was taken...
Summary BACKGROUND The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has posed great threat to human health, which been declared a public health emergency international concern (PHEIC) the WHO. T cells play critical role in antiviral immunity but their numbers and functional state COVID-19 patients remain largely unclear. METHODS We retrospectively reviewed counts total cells, CD4 + , CD8 cell subsets, serum cytokine concentration...
Abstract Exacerbation of macrophage-mediated inflammation contributes to pathogenesis various inflammatory diseases, but the immunometabolic programs underlying regulation macrophage activation are unclear. Here we show that V-set immunoglobulin-domain-containing 4 (VSIG4), a B7 family-related protein is expressed by resting macrophages, inhibits in response lipopolysaccharide. Vsig4 −/− mice susceptible high-fat diet-caused obesity and murine hepatitis virus strain-3 (MHV-3)-induced...
Abstract Background Thymosin alpha 1 (Tα1) had been used in the treatment of viral infections as an immune response modifier for many years. However, clinical benefits and mechanism Tα1 COVID-19 patients are still unclear. Methods We retrospectively reviewed outcomes 76 severe cases admitted to 2 hospitals Wuhan, China, from December 2019 March 2020. The thymus output peripheral blood mononuclear cells was measured by T-cell receptor excision circles (TRECs). levels exhaustion markers...
[Abstract] Objective Coronavirus disease 2019 (COVID-19) has become pandemic in the world. The need for IgG-IgM combined antibody test is booming, but data on diagnostic indexes evaluation was inadequate. aim of this study to evaluate a rapid SARS-CoV-2. Methods A total 179 patients were enrolled. Serum collected and corresponding nasal pharyngeal swab specimens SARS-CoV-2 RT-PCR. According RT-PCR results, under categorized as PCR positive group 90 negative 89 patients. Results 1. Of...
While lymphocytopenia is a common characteristic of coronavirus disease 2019 (COVID-19), the mechanisms responsible for this lymphocyte depletion are unclear. Here, we retrospectively reviewed clinical and immunological data from 18 fatal COVID-19 cases, results showed that these patients had severe lymphocytopenia, together with high serum levels inflammatory cytokines (IL-6, IL-8 IL-10), elevation many other mediators in routine laboratory tests, including C-reactive protein, lactate...
Coronavirus disease 2019 (COVID-19) has spread rapidly worldwide from Wuhan. An easy-to-use index capable of the early identification inpatients who are at risk becoming critically ill is urgently needed in clinical practice. Hence, aim this study was to explore an nomogram and a model triage patients into categories determine likelihood developing critical illness.A retrospective cohort conducted. We extracted data 84 with laboratory-confirmed COVID-19 one designated hospital. The primary...
Viral fulminant hepatitis (FH) is a severe disease with high mortality resulting from excessive inflammation in the infected liver. Clinical interventions have been inefficient due to lack of knowledge for inflammatory pathogenesis virus-infected We show that wild-type mice murine virus strain-3 (MHV-3), model viral FH, manifest and association significant elevation IL-1β expression serum Whereas, infection receptor-I deficient (IL-1R1-/-) or IL-1R antagonist (IL-1Ra) treated mice,...
The outbreak of highly contagious coronavirus disease 2019 (COVID-19) has posed a serious threat to human life and health, especially for those with underlying diseases. However, the impact COVID-19 epidemic on hemodialysis (HD) centers HD patients not been reported.
Glioblastoma (GBM) is adults' most common and fatally malignant brain tumor. The heterogeneity the leading cause of treatment failure. However, relationship between cellular heterogeneity, tumor microenvironment, GBM progression still elusive.Integrated analysis single-cell RNA sequencing (scRNA-seq) spatial transcriptome (stRNA-seq) were conducted to analyze microenvironment. We investigated subpopulation cells through gene set enrichment analyses, cell communications pseudotime analyses....
<h3>Importance</h3> Coronavirus disease 2019 (COVID-19) has been recognized as a pandemic by the World Health Organization. Whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can also infect tissues besides system, such ocular tissues, remains unclear. <h3>Objective</h3> To determine whether SARS-CoV-2 exists intracellularly in of patient previously infected with COVID-19. <h3>Design, Setting, and Participants</h3> This case study analyzed COVID-19 who had an glaucoma...
Excessive proliferation and activation of B cells, resulting in the production various autoantibodies, is a crucial link significant feature pathogenesis systemic lupus erythematosus (SLE), as well pathological basis multiorgan damage. However, whether exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-Exo) are involved immune regulation SLE has not been clarified. Therefore, our study aimed to investigate efficacy hucMSCs-Exo for treating SLE. peripheral blood...
Abstract Exosomes are membrane‐enclosed nanovesicles that shuttle active cargoes, such as circular RNAs (circRNAs) and microRNAs (miRNAs), between different cells. Human umbilical cord‐derived mesenchymal stem cells (Hu‐MSCs) can migrate to tumor sites exert complex functions throughout progression. In this study, we successfully isolated Hu‐MSCs from human cords based on their surface marker expression. Hu‐MSC‐derived exosomes significantly reduced the invasion, migration, proliferation of...
Stromal cell derived factor-1 (SDF-1) is a chemokine that plays critical role in the homing of stem and progenitor cells, including endothelial cells (EPCs). However, little research has been undertaken to evaluate roles SDF-1 biological functions EPCs related signaling pathways. The present study aimed investigate response SDF-1, as well underlying mechanisms. effects treatment on EPC proliferation, migration tube formation were assessed by performing MTS, Transwell vitro assays,...
Introduction. Caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, disease 2019 (COVID-19) has threatened global public health. Immune damage mechanisms are essential guidelines for clinical treatment and immune prevention.Hypothesis. The dysregulated type I interferon (IFN-I) responses, lymphocytopenia hypercytokinemia during SARS-CoV-2 infection have been reported. However, whether there is a correlation between levels of IFN-I severity COVID-19 not...
IQ motif containing GTPase activating protein 1 (IQGAP1) is a scaffold protein, which aberrantly expressed in several tumor types and closely associated with the development, metastasis prognosis of cancer. Several studies have demonstrated that IQGAP1 has broad prospects basic clinical research tumors. The present study aimed to explore effects IQGAP1‑small interfering (si) NA on proliferation U251 U373 glioma cell lines, markedly IQGAP1. human lines (U251 U373) were transfected siRNA...
Detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a supplemental option for reverse transcription polymerase chain reaction (RT-PCR) detection, regardless whether it immunoglobulin M (IgM), G (IgG) or combined detection. However, there are inadequate data on the sensitivity and specificity IgM/IgG which paramount importance decision whether, when, how to use antibody The aim this study was evaluate sensitivity, specificity, accuracy, positive...
Abstract Renal fibrosis is controlled by profibrotic and antifibrotic forces. Exploring anti-fibrosis factors mechanisms an attractive strategy to prevent organ failure. Here we identified the JNK-associated leucine zipper protein (JLP) as a potential endogenous factor. JLP, predominantly expressed in renal tubular epithelial cells (TECs) normal human or mouse kidneys, was downregulated fibrotic kidneys. Jlp deficiency resulted more severe unilateral ureteral obstruction (UUO) mice, while...
Stem cell therapy is a promising strategy for the treatment of traumatic brain injury (TBI). However, animal experiments are needed to evaluate safety; in particular, examine immunogenicity and tumorigenicity human umbilical cord mesenchymal stem cells (huMSCs) before clinical application. In this study, huMSCs were harvested from amniotic membrane vascular tissue. A rat model TBI was established using controlled cortical impact method. Starting third day after injury, rats injected with 10...