A5110823092- Cancer, Hypoxia, and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Dental Implant Techniques and Outcomes
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Nanoplatforms for cancer theranostics
- Muscle Physiology and Disorders
- Genomics and Rare Diseases
- Silk-based biomaterials and applications
- Cancer Cells and Metastasis
- Microencapsulation and Drying Processes
- Food composition and properties
- Amino Acid Enzymes and Metabolism
- Circular RNAs in diseases
- Enzyme-mediated dye degradation
- Ferroptosis and cancer prognosis
- Pluripotent Stem Cells Research
- Microbial Inactivation Methods
- Genomic variations and chromosomal abnormalities
- Tea Polyphenols and Effects
- 14-3-3 protein interactions
Zhejiang University
2022-2025
Stomatology Hospital
2022-2025
Nanjing Medical University
2020-2025
Xi’an Jiaotong-Liverpool University
2024
University of the Pacific
2019-2024
Shanghai Jiao Tong University
2022-2024
Shanghai Ninth People's Hospital
2024
Anhui Medical University
2024
Ruijin Hospital
2021-2023
University of Wisconsin–Madison
2022
PurposeDespite the recognized clinical value of exome-based diagnostics, methods for comprehensive genomic interpretation remain immature. Diagnoses are based on known or presumed pathogenic variants in genes already associated with a similar phenotype. Here, we extend this paradigm by evaluating novel bioinformatics approaches to aid identification new gene–disease associations.MethodsWe analyzed 119 trios identify both diagnostic genotypes and candidate genes. We considered qualifying...
The mechanical microenvironment regulated by cancer-associated fibroblasts (CAFs) influence tumor progression. Chemotherapeutic interventions including 5-Fluorouracil (5-Fu) are commonly used for primary treatment of patients with advanced gastric cancer (GC), and the development acquired resistance to 5-Fu limits clinical efficacy these chemotherapies. However, if how interplay between CAFs regulates GC response is poorly understood. In this study, we demonstrate that high-level expression...
Intramuscular injection of plasmid is a potential alternative to viral vectors for the transfer therapeutic genes into skeletal muscle fibers. The low efficiency plasmid-based gene can be enhanced by electroporation (EP) coupled with intramuscular application hyaluronidase. We have investigated several factors that influence transfer. These include electrical parameters EP, optimal use hyaluronidase, age and strain host, size. Muscles very young mature normal, mdx, immunodeficient mice were...
Abstract Exosomes are membrane‐enclosed nanovesicles that shuttle active cargoes, such as circular RNAs (circRNAs) and microRNAs (miRNAs), between different cells. Human umbilical cord‐derived mesenchymal stem cells (Hu‐MSCs) can migrate to tumor sites exert complex functions throughout progression. In this study, we successfully isolated Hu‐MSCs from human cords based on their surface marker expression. Hu‐MSC‐derived exosomes significantly reduced the invasion, migration, proliferation of...
Abstract Aims To investigate the clinical and radiographic outcomes of a chemically modified sandblasted large‐grit acid‐etched implant (hydrophilic) in lateral sinus floor elevation (LSFE), compared with conventional one (hydrophobic). Materials Methods A retrospective study design was adopted. Patients who received LSFE simultaneous placement were recruited. According to different types surfaces, patients divided into two groups (the hydrophilic group hydrophobic group). Implant survival...
Treatment of osteochondral (OC) defects is still an immense challenge in clinic due to lack satisfactory strategy simultaneously regenerate both cartilage and subchondral bone. Herein, we propose effective bottom-up by developing a type multifunctional chitosan-based nanocomposite microgels as the building blocks repair OC defects. The are biocompatible, biodegradable, have hierarchical nanofibrous structure, mimicking structural feature extracellular matrix (ECM) facilitate cell attachment...
ABSTRACT Aim To propose a modified method for establishing peri‐implantitis murine model, assess the gene expression profile and immune cell infiltration of gingiva alveolar bone, evaluate transcriptomic similarity between patients with corresponding model. Materials Methods A ligature‐induced model was established using an immediate implant placement approach. RNA sequencing performed to determine profiles peri‐implant tissues from mice, healthy individuals. Histopathological bioinformatics...
Triple-negative breast cancer (TNBC) is one of the most challenging malignancies with highest mortality rates among women. TNBC relies on both amino acid metabolism and glycolysis to fuel its bioenergetic biosynthetic demand. However, potential crosstalk between these two metabolic pathways impact progression remains largely unexplored. In this study, we observed that SLC7A5, a key transporter, was upregulated in strongly associated poor patient prognosis. We demonstrated elevated SLC7A5...
Dystrophin gene transfer using helper-dependent adenoviral vectors (HDAd) deleted of all viral genes is a promising option to treat muscles in Duchenne muscular dystrophy (DMD). Previously, we reported high-level dystrophin expression and functional correction dystrophin-deficient (mdx) mouse muscle 60 days after with an HDAd encoding two full-length murine cDNAs (referred as HDCBDysM). In the present study, tested long-term efficacy HDCBDysM by examining contractility parameters stability 1...
Dimethyl sulfide (DMS) emitted from the petrochemical industry is a typical volatile organic sulfur compound (VOSC) with poor solubility and odorous smell. A microbial fuel cell (MFC) regarded as an appreciative method for simultaneous DMS degradation electricity generation. In this work, first time, we developed MFC system treatment. With initial concentration of 95 mg L–1, removal efficiency at closed-circuit state was achieved high 89.5% within 40 h, which 1.21 times higher than that...
Arsenic was recently identified as a pollutant that is major cause of lung cancer. Since heparin-binding EGF-like growth factor (HB-EGF) reported to be promising therapeutic target for cancer, we investigated the role and mechanism HB-EGF during arsenic-induced carcinogenesis development expression were upregulated in As-T cells, cancer cell lines, most tissue samples; activated EGFR/p-ERK/HIF-1α pathway induced VEGF by regulating HIF-1α transcription. transcriptional stimulation facilitated...
Duchenne muscular dystrophy is caused by a genetic defect in the dystrophin gene. The absence of results muscle fiber necrosis and regeneration, leading to progressive loss. Utrophin close analogue dystrophin. A substantial, ectopic expression utrophin extrasynaptic sarcolemma dystrophin-deficient fibers can prevent deleterious effects deficiency. An alternative approach for up-regulation involves augmentation transcription via endogenous promoter using custom-designed transcriptional...
Rare monogenic disorders often share molecular etiologies involved in the pathogenesis of common diseases. Congenital glycosylation (CDG) and deglycosylation (CDDG) are rare pediatric with symptoms that range from mild to life threatening. A biological mechanism shared among CDG CDDG as well more neurodegenerative diseases such Alzheimer's disease amyotrophic lateral sclerosis, is endoplasmic reticulum (ER) stress. We developed isogenic human cellular models two types only known discover...
Possible myogenic differentiation of SSEA-1- and OCT-4-positive murine embryonic stem cells (ESCs) embryoid bodies (EBs) was studied in vitro vivo. In vitro, ESC- or EB-derived ESCs (EBs/ESCs) showed only traces Pax 3 7 expression by immunocytochemistry immunoblot. By RT-PCR, determinant molecules (myf5, myoD, myogenin) were expressed EBs/ESCs but not ESCs. However, such cultures, very rare contracting myotubes still present. Suspensions LacZ-labeled EBs injected into anterior tibialis...
N-propanol is a vital flavor compound of Chinese baijiu, and the proper n-propanol contents contribute to rich baijiu. However, excessive content in liquor will reduce drinking comfort. Based on Box–Behnken design principle, response surface test was used optimize factors affecting production simulated liquid state fermentation best combination determined. Results showed that ratio additional glucose threonine temperature had significant effect (p = 0.0009 < 0.01 p 0.0389 0.05,...
Hepatocellular carcinoma (HCC) is a highly aggressive malignant disease, and numerous studies have shown that certain functional long noncoding RNAs (lncRNAs) are implicated in the progression of several cancers. The purpose research was to determine, using database, bioinformatics, statistical analysis, whether or not lncRNA PRR7-AS1 (PRR7-AS1) related HCC. TCGA datasets were used conduct on expression pattern In order evaluate efficacy GIHCG as prognostic tool, both survival Cox regression...
Non-small cell lung cancer (NSCLC) is a highly lethal disease with complex and heterogeneous tumor microenvironment. Currently, common animal models based on subcutaneous inoculation of suspensions do not recapitulate the microenvironment in NSCLC. Herein we describe murine orthotopic xenograft model that employs intrapulmonary three-dimensional multicellular spheroids (MCS). Specifically, fluorescent human NSCLC cells (A549-iRFP) were cultured low-attachment 96-well microplates collagen for...
ABSTRACT The increasing contamination of agricultural soils with cadmium (Cd) poses a significant threat to human health and global food security. Plants initiate series mechanisms reduce Cd toxicity. However, the response maize toxicity remains poorly understood. In this study, we identified that ZmHMT3 , which encodes homocysteine S‐methyltransferases family protein, acted as regulator tolerance in maize. Subcellular localization situ PCR exhibited was localized cytoplasm predominantly...