A5059900841- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Biochemical and Molecular Research
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Viral-associated cancers and disorders
- Amino Acid Enzymes and Metabolism
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Immune cells in cancer
- Minimally Invasive Surgical Techniques
- Cancer-related gene regulation
- Cytomegalovirus and herpesvirus research
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Chemokine receptors and signaling
- Enzyme Production and Characterization
Wuhan University
2014-2025
National Institute of Allergy and Infectious Diseases
2023
Guangdong University of Technology
2019
Ganzhou People's Hospital
2016
Abstract The proper regulation of transcription is essential for maintaining genome integrity and executing other downstream cellular functions 1,2 . Here we identify a stable association between the genome-stability regulator sensor single-stranded DNA (SOSS) 3 Integrator-PP2A (INTAC) 4–6 Through SSB1-mediated recognition DNA, SOSS–INTAC stimulates promoter-proximal termination attenuates R-loops associated with paused RNA polymerase II to prevent R-loop-induced instability....
An R loop is a unique triple-stranded structure that participates in multiple key biological processes and relevant to human diseases. Accurate comprehensive profiling prerequisite for loops studies. However, current mapping methods generate large discrepancies, therefore an independent method urgent need. Here, we establish CUT&Tag (Tn5-based cleavage under targets tagmentation) by combining GST-His6-2×HBD (glutathione S-transferase-hexahistidine-2× hybrid-binding domain), artificial...
G-quadruplexes (G4s) are noncanonical DNA secondary structures formed through the self-association of guanines, and G4s distributed widely across genome. G4 participates in multiple biological processes including gene transcription, G4-targeted ligands serve as potential therapeutic agents for DNA-targeted therapies. However, genome-wide studies exact roles transcriptional regulation still lacking. Here, we establish a sensitive G4-CUT&Tag method profiling native with high resolution...
Paternally expressed imprinted gene 10 (PEG10), derived from the Ty3/Gypsy family of retrotransposons, has been implicated as a genetic gene. Accumulating evidence suggests that PEG10 plays an important role in tumor growth various cancers, including hepatocellular carcinoma, lung cancer and prostate cancer. However, correlation between breast remains unclear. In present study, we evaluated characterized human proliferation, cell cycle, clone formation, migration invasion. The expression...
Cyclin-dependent kinase 12 (CDK12) interacts with cyclin K to form a functional nuclear that promotes processive transcription elongation through phosphorylation of the C-terminal domain RNA polymerase II (Pol II). To gain comprehensive understanding CDK12's cellular function, we used chemical genetic and phosphoproteomic screening identify landscape human CDK12 substrates, including regulators transcription, chromatin organization, splicing. We further validated LEO1, subunit...
Adult T-cell acute lymphoblastic leukemia (T-ALL) is a refractory leukemia. We previously showed that CCL25/CCR9 promotes T-ALL metastasis. In the present study, we assessed effects of CCL25 on Wnt expression and Wnt5a PI3K/Akt RhoA activation. Transwell assays mouse xenograft experiments were utilized to assess MOLT4 cell invasion, migration The actin polarization pseudopodium formation examined using laser scanning confocal microscopy electron microscopy. induced in cells by promoting...
Natural fiber reinforced polymer-based composites have been growing into a type of green composites. The properties natural are closely related to the structure fibers. Bagasse (BF) is one most used fibers for preparing However, few examples previous research touch on quantitatively characterization BF and its effect In this work, four kinds including untreated (UBF), alkali treated (ABF), modified by silane coupling agent (SBF), combining treatment with (ASBF) were prepared melting blended...
MYCN amplification in neuroblastoma leads to aberrant expression of oncoprotein, which binds active genes promoting transcriptional amplification. Yet, how coordinates transcription elongation meet productive and machinery represents MYCN-driven vulnerability remain be identified. We conducted a targeted screen factors identified the super complex (SEC) as unique MYCN-amplified neuroblastomas. directly EAF1 recruits SEC enhance processive elongation. Depletion or AFF1/AFF4, another core...
Abstract Tumor cells must rewire nucleotide synthesis to satisfy the demands of unbridled proliferation. Meanwhile, they exhibit augmented reactive oxygen species (ROS) production which paradoxically damages DNA and free deoxy-ribonucleoside triphosphates (dNTPs). How these metabolic processes are integrated fuel tumorigenesis remains be investigated. MYC family oncoproteins coordinate ROS generation drive development numerous cancers. We herein perform a Clustered Regularly Interspaced...
G-quadruplexes (G4s) are unique noncanonical nucleic acid secondary structures, which have been proposed to physically interact with transcription factors and chromatin remodelers regulate cell type-specific transcriptome shape landscapes.
The ability of human cells to defend against viruses originating from distant species has long been ignored. Owing the pressure natural evolution and exploration, some these may be able invade beings. If their 'fresh' host had no defences, could cause a serious pandemic, as seen with HIV, SARS (severe acute respiratory syndrome) avian influenza virus that originated chimpanzees, common palm civet birds, respectively. It is unknown whether immune system tolerate invasion plant virus. To model...
We have previously demonstrated that the CCR9/CCL25 signaling pathway plays an important role in drug resistance human acute T-lymphocytic leukemia (T-ALL) by inducing activation of ERM protein with polarized distribution T-ALL cell line MOLT4. However, mechanism action activated MOLT4 cells induced CCL25 remains uncharacterized. Here we investigated CCR9/CCL25-initiated CCR9-high-expressing cells. Our results showed 1) function P-gp was increased after treatment CCL25; 2) colocalized and...
The oncogene steroid receptor coactivator-3 (SRC-3) plays a pivotal role in the downstream transcriptional regulation mediated by estrogen (ER), thereby promoting occurrence and progression of endocrine resistance breast cancer. Herein, we disclose novel series potent SRC-3 PROTACs to overcome resistance. These were able efficiently degrade inhibit proliferation wild-type endocrine-resistant cancer cells. Notably, compound BY13 could significantly growth drug-resistant tumors without...
The source of IgA and the mechanism for deposition in mesangium remain unknown primary nephropathy. Because CD19+CD5+ B cells are important producers contribute to several autoimmune diseases, they may play an role In this study, flow cytometry, quantitative PCR, confocal microscopy were used assess frequency, distribution, Ig production, CD phenotypes, cytokine sensitivity apoptosis peripheral blood, peritoneal fluid, kidney biopsies 36 patients with All nephropathy significantly more...
The epithelial–mesenchymal transition (EMT) is a fundamental process governing morphogenesis in multicellular organisms and has recently been implicated promoting carcinoma invasion metastasis. Besides their therapeutic effects, accumulating evidences suggest that chemotherapeutic agents also induced EMT enhanced the malignancy of treated cancer cells; however, mechanism(s) still remains unclear. Here, we investigated role β-catenin signaling doxorubicin (Dox)-induced human gastric cell line...
Abstract Metastatic disease remains the primary cause of death for individuals with T cell acute lymphoblastic leukemia (T‐ALL). microRNAs (miRNAs) play important roles in pathogenesis T‐ALL by inhibiting gene expression at posttranscriptional levels. The goal current project is to identify any significant miRNAs metastasis. We observed miR‐146b‐5p be downregulated patients and lines, bioinformatics analysis implicated hematopoietic system. inhibited migration invasion cells. Interleukin‐17A...
Mucosa-associated T-lymphocyte (MAIT) cells that are selectively accumulated in the intestinal mucosa may be involved immune regulation. MAIT determined by their Vα7.2-Jα33 (human)/Vα19-Jα33 (mice) invariant chain. The encoding DNA sequences of Human Jα33 and mouse identical. In order to study role IBD we produced anti-Jα33 antibody detect TNBS induced models.Colitis was male BALB/c mice. Jα33+MAIT from normal mice were transferred into as donors. Colitis severity evaluated clinically...
Background: ZWINT is a crucial component of the mitotic checkpoint. However, its possible role in lung cancer unclear. In this study, we determined correlation with cancer. Methods: Real-time PCR and immunohistochemistry (IHC) were used to determine 40 collected clinical samples. Chi-square test was examine correlations between expression clinicopathological factors. The prognostic significance mRNA evaluated using Kaplan–Meier plotter. Univariate multivariate Cox proportional hazards...
The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance metastasis diverse solid tumors. We previously reported that 48 h treatment of anti-cancer doxorubicin could induce EMT human gastric cancer BGC-823 cells. However, the long term effects this transient were unknown. In study we found after with 0.1 μg/ml doxorubicin, most cells died next week, while minor population survived formed colonies. propagated...