A5068112614- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Anaerobic Digestion and Biogas Production
- Methane Hydrates and Related Phenomena
- Food Quality and Safety Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nematode management and characterization studies
- Botanical Research and Chemistry
- Antioxidant Activity and Oxidative Stress
- Algal biology and biofuel production
- Biochemical Acid Research Studies
- Insect and Pesticide Research
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Hydraulic Fracturing and Reservoir Analysis
- Polyamine Metabolism and Applications
- Geoscience and Mining Technology
- Plant-Microbe Interactions and Immunity
- Biochemical and Molecular Research
- Bacterial biofilms and quorum sensing
University of South Florida
2021-2023
Rice University
2018-2021
Wuhan University
2011-2016
Pennsylvania State University
2016
Huazhong Agricultural University
2007-2011
Shengli Oilfield Central Hospital
1994
ABSTRACT Approaches using metabolic engineering and synthetic biology to overproduce terpenoids, such as the precursors of taxol artemisinin, in microbial systems have achieved initial success. However, due lack steady‐state kinetic information incomplete understanding terpenoid biosynthetic pathway, it has been difficult build a highly efficient, universal system. Here, we reconstituted mevalonate pathway produce farnesene (a precursor new jet fuel) vitro purified protein components. The...
Microbial fatty acid derivatives are emerging as promising alternatives to fossil fuel derived transportation fuels. Among bacterial synthases (FAS), the Escherichia coli FAS is perhaps most well studied, but little known about its steady-state kinetic behavior. Here we describe reconstitution of E. using purified protein components and report detailed analysis this reconstituted system. When all ketosynthases present at 1 μM, maximum rate free synthesis exceeded 100 μM/ min. The turnover...
Energy from remote methane reserves is transformative; however, unintended release of this potent greenhouse gas makes it imperative to convert efficiently into more readily transported biofuels. No pure microbial culture that grows on anaerobically has been isolated, despite capture through anaerobic processes efficient than aerobic ones. Here we engineered the archaeal methanogen Methanosarcina acetivorans grow as a and biofuel precursor acetate. To methane, cloned enzyme methyl-coenzyme M...
Abstract Formate can be envisioned at the core of a carbon-neutral bioeconomy, where it is produced from CO 2 by (electro-)chemical means and converted into value-added products enzymatic cascades or engineered microbes. A key step in expanding synthetic formate assimilation its thermodynamically challenging reduction to formaldehyde. Here, we develop two-enzyme route which activated formyl phosphate subsequently reduced Exploiting promiscuity acetate kinase N- acetyl-γ-glutamyl reductase,...
ABSTRACT The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high‐value oleo chemicals. As Saccharomyces cerevisiae has many advantages industrial production compared to Escherichia coli . Here, we attempted engineer overproduction of acids. First, disruption the beta‐oxidation pathway, elimination acyl‐CoA synthetases, overexpression different thioesterases acetyl‐CoA carboxylase ACC1, engineering supply acetyl‐CoA....
Abstract As a highly valued keto‐carotenoid, astaxanthin is widely used in nutritional supplements and pharmaceuticals. Therefore, the demand for biosynthetic improved efficiency of biosynthesis has driven investigation metabolic engineering native producers heterologous hosts. However, microbial resources are limited. In this study, we found that α‐Proteobacterium Sphingomonas sp. ATCC 55669 could produce naturally. We whole‐genome sequencing to identify pathway using combined...
ABSTRACT We previously demonstrated anaerobic conversion of the greenhouse gas methane into acetate using an engineered archaeon that produces methyl‐coenzyme M reductase (Mcr) from unculturable microorganisms a microbial mat in Black Sea to create first culturable prokaryote reverses methanogenesis and grows anaerobically on methane. In this work, we further same host with goal converting butanol. Instead, discovered process for secreted valuable product, L ‐lactate, sufficient optical...
One-carbon (C1) compounds are emerging as cost-effective and potentially carbon-negative feedstocks for biomanufacturing, which require efficient, versatile metabolic platforms the synthesis of value-added products. Synthetic formyl-CoA elongation (FORCE) pathways allow diverse product from C1 via iterative elongation, operating independently host metabolism with reduced engineering complexity improved theoretical yields. However, a major bottleneck was identified suboptimal kinetics core...
Abstract It is of great economic interest to produce succinate from low‐grade carbon sources, which can enhance the competitiveness biological route. In this study, producer Escherichia coli CT550/pHL413KF1 was further engineered efficiently use mixed sugars non‐food based soybean hydrolysate under anaerobic conditions. Since many common E. strains fail galactose anaerobically even if they it aerobically, glucose, and related sugar transporters were deactivated individually evaluated. The...
Abstract It is of great economic interest to produce succinate from low‐grade carbon sources, which can make it more economically competitive against petrochemical‐based succinate. Galactose sugars constitute a significant fraction the soluble carbohydrate in meal agricultural sources considered low value or waste byproduct oilseed processing. To improve galactose utilization, effect galR and glk on uptake was investigated by deactivation each gene three previously engineered host strains....
The chemical 3-hydroxypropionate (3HP) is an important starting reagent for the commercial synthesis of specialty chemicals. In this study, a part 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula was utilized 3HP production. To study basic biochemistry pathway, in vitro-reconstituted system established using acetyl-CoA as substrate kinetic analysis system. results indicated that formation sensitive to carboxylase and malonyl-CoA reductase, but not malonate semialdehyde...
Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there no report that B. the adults of long-horned beetle, Batocera horsfieldi, a pest poplar trees. This work aims select strain towards Asian beetle.A total 504 strains were tested for insecticidal activity horsfieldi by artificial feeding. A ZQ-89 was found with high toxicity adults. The rectified lethal rate beetle 55·33%. Additionally, body weight and egg-hatching respectively, decreased 2·22 19·62%...