A5082711272- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Diet and metabolism studies
- Mass Spectrometry Techniques and Applications
- Child and Adolescent Psychosocial and Emotional Development
- Analytical Chemistry and Chromatography
- X-ray Spectroscopy and Fluorescence Analysis
- Nuclear physics research studies
- Nuclear Physics and Applications
- Liver Disease Diagnosis and Treatment
- Vitamin D Research Studies
- Pharmacogenetics and Drug Metabolism
- Adipose Tissue and Metabolism
- Drug Transport and Resistance Mechanisms
- Cancer, Hypoxia, and Metabolism
- Attachment and Relationship Dynamics
- Per- and polyfluoroalkyl substances research
- Toxic Organic Pollutants Impact
- Tryptophan and brain disorders
- Birth, Development, and Health
- Fungal Biology and Applications
- Insect and Arachnid Ecology and Behavior
- Entomopathogenic Microorganisms in Pest Control
- Heme Oxygenase-1 and Carbon Monoxide
- Crystallography and Radiation Phenomena
Pennsylvania State University
2013-2022
Karolinska University Hospital
2022
Karolinska Institutet
2022
University of Prince Edward Island
2015-2017
Joint Center for Structural Genomics
2017
University of Maryland, Baltimore
1991-2017
University of Ottawa
2015
Children's Hospital Research Institute of Manitoba
2015
University of Manitoba
2015
Government of Manitoba
2015
BackgroundAlteration of the gut microbiota through diet and environmental contaminants may disturb physiological homeostasis, leading to various diseases including obesity type 2 diabetes. Because most exposure environmentally persistent organic pollutants (POPs) occurs diet, host gastrointestinal tract commensal are likely be exposed POPs.ObjectivesWe examined effect 2,3,7,8-tetrachlorodibenzofuran (TCDF), a contaminant, on metabolism, we correlations between composition signaling...
Commensal microbiota-dependent tryptophan catabolism within the gastrointestinal tract is known to exert profound effects upon host physiology, including maintenance of epithelial barrier and immune function. A number abundant microbiota-derived metabolites exhibit activation potential for aryl hydrocarbon receptor (AHR). Gene expression facilitated by AHR through presence dietary or microbiota-generated can influence homeostasis confer protection from intestinal challenges. Utilizing...
Bile acids are potent antibacterial compounds and play an important role in shaping the microbial ecology of gut. Here, we combined flow cytometry, growth rate measurements (OD600), NMR- mass spectrometry-based metabolomics to systematically profile impact bile on microbiome using vitro vivo models. This study confirmed that (1) unconjugated possess more activity than conjugated acids; (2) Gram-positive bacteria sensitive Gram-negative bacteria; (3) some probiotic such as Lactobacillus...
48 12-month-old infants and their mothers were videotaped in the Ainsworth Strange Situation. Each infant-mother dyad was also filmed for 3 min while mother completed a questionnaire infant left to explore room devoid of toys, situation which maternal compliance with request complete expected compete attentional demands made on her by infant. Infant-mother attachment classified as secure, anxious-avoidant, or anxious-resistant basis behavior Assessment sensitivity during included behaviors...
Abstract Lung cancer remains the most common cause of deaths worldwide, yet there is currently a lack diagnostic noninvasive biomarkers that could guide treatment decisions. Small molecules (<1,500 Da) were measured in urine collected from 469 patients with lung and 536 population controls using unbiased liquid chromatography/mass spectrometry. Clinical putative prognostic validated by quantitation normalized to creatinine levels at two different time points further confirmed an...
In the present study we have examined effects of androgens and estrogens on circulating arginine vasopressin (AVP). Adult male Wistar rats had serum AVP levels 0.4 μU/ml. Two weeks after bilateral castration, rose to 2.6/nU/ml, but daily testosterone administration (100 μg/100 g BW) castrate males prevented increase (0.8 μU/ml). During a normal estrous cycle, adult female values 0.6 μU/ml during diestrus, 4.6 morning proestrus, 1.3 afternoon 1.5 day estrus. These changes in paralleled...
Energy from remote methane reserves is transformative; however, unintended release of this potent greenhouse gas makes it imperative to convert efficiently into more readily transported biofuels. No pure microbial culture that grows on anaerobically has been isolated, despite capture through anaerobic processes efficient than aerobic ones. Here we engineered the archaeal methanogen Methanosarcina acetivorans grow as a and biofuel precursor acetate. To methane, cloned enzyme methyl-coenzyme M...
The gut microbiota modulates obesity and associated metabolic phenotypes in part through intestinal farnesoid X receptor (FXR) signaling. Glycine-β-muricholic acid (Gly-MCA), an FXR antagonist, has been reported to prevent or reverse high-fat diet (HFD)-induced genetic obesity, insulin resistance, fatty liver; however, the mechanism by which these are improved is not fully understood. current study investigated influence of activity on community structure function its impact hepatic lipid...
A compelling demonstration of adaptation by natural selection is the ability parasites to manipulate host behavior. One dramatic example involves fungal species from genus Ophiocordyceps that control their ant hosts inducing a biting Intensive sampling across globe ants died after being manipulated suggests this phenomenon highly species-specific. We advance our understanding system reconstructing manipulation under controlled laboratory conditions and combining with field observations...
Although oral methotrexate (MTX) remains the anchor drug for rheumatoid arthritis (RA), up to 50% of patients do not achieve a clinically adequate outcome. In addition, there is lack prognostic tools treatment response prior initiation. This study was undertaken investigate whether interindividual differences in human gut microbiome can aid prediction MTX efficacy new-onset RA.
To determine the effect of microbiota on vitamin D metabolism, serum 25hydroxyvitamin D(25D), 24,25dihydroxyvitamin (24,25D) and 1,25dihydroxyvitamin (1,25D) were measured in germfree (GF) mice before after conventionalization (CN). GF had low levels 25D, 24,25D 1,25D hypocalcemic. CN with microbiota, for 2wks recovered levels. Females more 25D than males both as CN. Introducing a limited number commensals (8 commensals) increased to same extent Monocolonization enteric pathogen Citrobacter...
A negative ion electrospray ionization tandem mass spectrometric technique was developed for the analysis of glycerophospholipids. Examination product spectrum deprotonated molecular provided sufficient information to identify both class glycerophospholipid and weights two fatty acid moieties. This applied profiling glycerophospholipids present in chloroform/methanol extracts four different bacterial species. The principal phospholipids detected by this were phosphatidylglycerols...
Abstract Cationic and anionic electrospray mass spectra were measured for equine myoglobin, lactalbumin hen egg white lysozyme from acidic basic solutions. Both positive negative ions detected all solutions in which the proteins soluble. This was observed both nebulization‐assisted thermally assisted electrospray. As other studies, distribution of charge states reflected functional groups proteins, also folding or extension protein.
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor. Activation of AhR mediates the expression target genes (e.g., CYP1A1 ) by binding to dioxin response element (DRE) sequences in their promoter region. To understand multiple mechanisms AhR-mediated gene regulation, microarray analysis on liver isolated from ligand-treated transgenic mice expressing wild-type (WT) Ahr or DRE-binding mutant (A78D) an ahr - null background was performed. Results revealed that DRE...
Environmental exposure to dioxins and dioxin-like compounds poses a significant health risk for human health. Developing better understanding of the mechanisms toxicity through activation aryl hydrocarbon receptor (AHR) is likely improve reliability assessment. In this study, AHR-dependent metabolic response mice exposed 2,3,7,8-tetrachlorodibenzofuran (TCDF) was assessed using global (1)H nuclear magnetic resonance (NMR)-based metabolomics targeted metabolite profiling extracts obtained...
We have previously demonstrated a role for the aryl hydrocarbon receptor (AHR) in attenuation of cholesterol biosynthesis pathway. This regulation did not require that AHR binds to its cognate response element. Based on these observations and other reports depicting lipid metabolism, we chose investigate involvement fatty acid synthesis pathway mice humans. For this purpose, C57BL/6J, liver-specific transgenic DRE-binding mutant AhR (A78D-AhrTtrCreAlbAhrfx/fx) CreAlbAhrfx/fx were treated...
Short chain fatty acids (SCFAs) are important regulators of host physiology and metabolism may contribute to obesity associated metabolic diseases. Interest in SCFAs has increased part due the recognized importance how production by microbiota signal host. Therefore, reliable, reproducible, affordable methods for SCFA profiling required accurate identification quantitation. In current study, four different (acetic acid, propionic butyric acid) extraction quantitation were compared using two...
Gamma rays emitted by excited ${\mathrm{Si}}^{28}$ formed in the reaction ${\mathrm{Al}}^{27}(p, \ensuremath{\gamma}){\mathrm{Si}}^{28}$ at ${E}_{p}=771$ kev have been selectively absorbed silicon. Selective, or resonant, absorption occurs when absorber is placed such an angle that loss of energy upon emission and reabsorption photons restored Doppler shift resulting from forward motion nucleus. A narrow slit a lead collimator filled with silicon powder, transmitted gamma radiation detected...