A5082173877- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Microbial bioremediation and biosurfactants
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Insect-Plant Interactions and Control
- Plant and animal studies
- Corrosion Behavior and Inhibition
- Enzyme Catalysis and Immobilization
- Anaerobic Digestion and Biogas Production
- Microbial Community Ecology and Physiology
- Biofuel production and bioconversion
- Escherichia coli research studies
- Bacillus and Francisella bacterial research
- Microbial Fuel Cells and Bioremediation
- Microbial metabolism and enzyme function
- Wastewater Treatment and Nitrogen Removal
- Forest Insect Ecology and Management
- Antimicrobial Peptides and Activities
- Phytoplasmas and Hemiptera pathogens
- Water Treatment and Disinfection
- Metalloenzymes and iron-sulfur proteins
- Insect and Arachnid Ecology and Behavior
Pennsylvania State University
2016-2025
University of Delaware
1999-2024
Institut National des Sciences Appliquées de Lyon
2023-2024
Institut des Nanotechnologies de Lyon
2023-2024
École d'Ingénieurs en Chimie et Sciences du Numérique
2023
Université Claude Bernard Lyon 1
2023
Centre National de la Recherche Scientifique
2023
École Centrale de Lyon
2023
Leiden University
2020
Utrecht University
2020
Interkingdom signaling is established in the gastrointestinal tract that human hormones trigger responses bacteria; here, we show corollary true, a specific bacterial signal, indole, recognized as beneficial signal intestinal epithelial cells. Our prior work has shown secreted by commensal Escherichia coli and detected feces, reduces pathogenic E. chemotaxis, motility, attachment to However, effect of indole on cells not known. Because are likely be exposed continuously hypothesized may for...
Phages are the most abundant entity in biosphere and outnumber bacteria by a factor of 10. Phage DNA may also constitute 20% bacterial genomes; however, its role is ill defined. Here, we explore impact cryptic prophages on cell physiology precisely deleting all nine prophage elements (166 kbp) using Escherichia coli. We find that contribute significantly to resistance sub-lethal concentrations quinolone β-lactam antibiotics primarily through proteins inhibit division (for example, KilR rac...
The cross-species bacterial communication signal autoinducer 2 (AI-2), produced by the purified enzymes Pfs (nucleosidase) and LuxS (terminal synthase) from S-adenosylhomocysteine, directly increased Escherichia coli biofilm mass 30-fold. Continuous-flow cells coupled with confocal microscopy corroborated these results showing addition of AI-2 significantly both thickness reduced interstitial space between microcolonies. As expected, to which lack ability transport (lsr null mutant) failed...
Abstract Background As a stationary phase signal, indole is secreted in large quantities into rich medium by Escherichia coli and has been shown to control several genes (e.g., astD , tnaB gabT ), multi-drug exporters, the pathogenicity island of E. ; however, its impact on biofilm formation not well-studied. Results Through series global transcriptome analyses, confocal microscopy, isogenic mutants, dual-species biofilms, we show here that non-toxic signal controls biofilms repressing...
ABSTRACT Biofilms are associated with a wide variety of bacterial infections and pose serious problem in clinical medicine due to their inherent resilience antibiotic treatment. Within biofilms, persister cells comprise small subpopulation that exhibits multidrug tolerance antibiotics without undergoing genetic change. The low frequency cell formation makes it difficult isolate study persisters, persistence is often attributed quiescent metabolic state induced by toxins regulated through...
ABSTRACT During infection in the gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) O157:H7 is exposed to a wide range of signaling molecules, including eukaryotic hormones epinephrine and norepinephrine, bacterial signal molecules such as indole. Since these have been shown be involved regulation phenotypes motility virulence that are crucial for EHEC infections, we hypothesized also govern initial recognition large intestine environment attachment host cell surface. Here,...
With the opportunistic pathogen Pseudomonas aeruginosa, quorum sensing based on homoserine lactones was found to influence biofilm formation. Here we discern a mechanism by which controls formation screening 5850 transposon mutants of P. aeruginosa PA14 for altered This screen identified PA3885 mutant, had 147-fold more than wild-type strain. Loss decreased swimming, abolished swarming, and increased attachment, although this did not affect production rhamnolipids. The mutant also wrinkly...
Summary Bacteria are often thought of as having two dormant phenotypes: the viable but non‐culturable (VBNC) state and persister state. Here we investigate relatedness stress‐induced phenotypes at single‐cell level examine cell morphology quantify resuscitation. Using classic starvation conditions to create VBNC cells, found that majority remaining Escherichia coli population spherical, have empty cytosol fail resuscitate; however, some spherical cells resuscitate immediately (most probably...
Summary Persister cells are a multi‐drug tolerant subpopulation of bacteria that contribute to chronic and recalcitrant clinical infections such as cystic fibrosis tuberculosis. Persisters metabolically dormant, so they highly all traditional antibiotics which mainly effective against actively growing cells. Here, we show the FDA ‐approved anti‐cancer drug mitomycin C ( MMC ) eradicates persister through growth‐independent mechanism. is passively transported bioreductively activated, leading...
Significance While the effects of gut microbes on brain development and function have been described, mechanisms remain largely unknown. Here, we report that tryptophan-metabolizing secrete indoles regulate neurogenesis in adult hippocampus. This stimulatory effect is mediated by metabolic- immune-linked aryl hydrocarbon receptor (AhR). Another AhR ligand, tryptophan metabolite kynurenine, failed to induce neurogenesis, suggesting ligand specificity AhR-mediated regulation neurogenesis. The...
The quorum-sensing disrupter (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) of the alga Delisea pulchra was found to inhibit swarming motility Escherichia coli completely at 13 microg cm-2 (also 20 ml-1) but did not its growth rate 13-52 or from 100 ml-1. Swimming inhibited by furanone 20-40 In addition, confocal scanning laser microscopy revealed that this 60 ml-1 biofilm formation E. coli, as it decreased thickness 55%, reduced number water channels and percentage live...
Analysis of the temporal development Escherichia coli K-12 biofilms in complex medium indicates greatest differential gene expression between biofilm and suspension cells occurred young at 4 7 h (versus 15 24 h). The main classes genes differentially expressed (biofilm versus cells) include 42 related to stress response (e.g. cspABFGI), 66 quorum sensing ydgG, gadABC, hdeABD), 20 motility flgBCEFH, fliLMQR, motB), 13 fimbriae sfmCHM, fimZ, csgC), sulfur tryptophan metabolism trpLBA, tnaLA,...
Subsocial behavior is the most primitive level of social interaction involving parents and offspring (79). Although preovipositional behaviors such as nest construction (48, 77) or various resource manipulations (10, 162) eventually benefit offspring, subsociality by definition restricted to postovipositional parental that promotes survival, growth, development (33). Insect adaptations comprise a continuum care rang ing from passive egg guarding an array complex grooming, feeding,...
ABSTRACT The use of plants for rehabilitation heavy-metal-contaminated environments is an emerging area interest because it provides ecologically sound and safe method restoration remediation. Although a number plant species are capable hyperaccumulation heavy metals, the technology not applicable remediating sites with multiple contaminants. A clever solution to combine advantages microbe-plant symbiosis within rhizosphere into effective cleanup technology. We demonstrated that expression...