A5024219837- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Essential Oils and Antimicrobial Activity
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Biochemical and Structural Characterization
- Antibiotic Use and Resistance
- Microbial Community Ecology and Physiology
- Antibiotics Pharmacokinetics and Efficacy
- Oral microbiology and periodontitis research
- Microbial Metabolism and Applications
- Infections and bacterial resistance
- Enzyme function and inhibition
- Medicinal Plants and Neuroprotection
- Microbial infections and disease research
- Protein Hydrolysis and Bioactive Peptides
- Advanced biosensing and bioanalysis techniques
Universidad Nacional Autónoma de México
2016-2025
Desert Research Center
2021
Universidad Autónoma de la Ciudad de México
2020
Universidad Autónoma Monterrey
2020
Colegio de Postgraduados
2015-2017
John Wiley & Sons (United States)
2016
Hudson Institute
2016
Instituto Nacional de Cardiología
2011-2015
Instituto Nacional de Cardiologia
2014
Secretaria de Salud
2013
DNA microarrays revealed that expression of ycfR, which encodes a putative outer membrane protein, is significantly induced in Escherichia coli biofilms and also by several stress conditions. We show deletion ycfR increased biofilm formation fivefold the presence glucose; glucose effect was corroborated showing binding cyclic AMP receptor protein to promoter. It appears YcfR multiple resistance since deleting rendered cell more sensitive acid, heat treatment, hydrogen peroxide, cadmium....
Acinetobacter baumannii is well adapted to hospital environments, and the persistence of its chronic infections mainly due ability form biofilms resistant conventional antibiotics host immune systems. Hence, inhibitions biofilm formation virulence characteristics provide other means addressing infections. In this study, antibiofilm activities twelve flavonoids were initially investigated. Three most active flavonoids, namely, fisetin, phloretin, curcumin, dose-dependently inhibited by a...
ABSTRACT For all bacteria, nearly every antimicrobial fails since a subpopulation of the bacteria enter dormant state known as persistence, in which antimicrobials are rendered ineffective due to lack metabolism. This tolerance antibiotics makes microbial infections leading cause death worldwide and treating chronic infections, including those wounds problematic. Here, we show that FDA‐approved anti‐cancer drug cisplatin [ cis ‐diamminodichloroplatinum(II)], mainly forms intra‐strand DNA...
Quorum quenching decreases Pseudomonas aeruginosa virulence factors and biofilm formation, alleviating infections in animal models. Nevertheless, it is usually performed laboratory strains such as PAO1 PA14, studies involving clinical or environmental isolates are scarce. In this work, the effects of ZnO nanoparticles, a potent quorum quencher for strain, were tested six from cystic fibrosis patients, furanone C-30 resistant strain urine, two PA14 gallium mutants, mutant four isolates....
We discovered previously that the small Escherichia coli proteins Hha (hemolysin expression modulating protein) and adjacent, poorly-characterized YbaJ are important for biofilm formation; however, their roles have been nebulous. Biofilms intricate communities in which cell signaling often converts single cells into primitive tissues. Here we show decreases formation dramatically by repressing transcription of rare codon tRNAs serves to inhibit fimbriae production some extent fimbrial genes...
The quorum-quenching compounds brominated furanone C-30 and 5-fluorouracil inhibit the pathogenicity of Pseudomonas aeruginosa laboratory strains PA01 PA14; however, there is no report studying effectiveness these for clinical isolates. Therefore, effect both quorum quenchers on production pyocyanin, elastase alkaline protease eight from children was evaluated. Although were in general effective attenuation factors, three resistant to found. For 5-fluorouracil, some isolates showed...
Quorum sensing (QS) coordinates the expression of multiple virulence factors in Pseudomonas aeruginosa; hence its inhibition has been postulated as a new alternative to treat infections. In particular, QS interference approaches claim that they attenuate bacterial without directly decreasing growth and suggest vivo immune system would control Moreover, since vitro experiments performed rich medium demonstrate interfering with decreases production affecting it was assumed than therapies will...
Does the understanding of dynamics biochemical networks in vivo , terms properties their components determined vitro require latter to be all under same conditions? An ‐like assay medium for enzyme activity determination was designed based on concentrations major ionic constituents E scherichia coli cytosol: K + N a M g 2+ phosphate, glutamate, sulfate and Cl − . The maximum capacities ( V max ) extracted enzymes two pathways were using both this specific each enzyme. activities differed...
Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by whole population bacteria, regardless whether they invested or not their production. These can be used QS defective mutants for growth since these do contribute to production, considered social cheaters. Pyocyanin is a phenazine toxic, QS-controlled metabolite produced P. aeruginosa. It redox-active compound promotes...
Abstract Pseudomonas aeruginosa is one of the most worrisome infectious bacteria due to its intrinsic and acquired resistance against several antibiotics recalcitrance infections; hence, development novel antimicrobials effective multidrug-resistant P. mandatory. In this work, silver nanoparticles obtained by green synthesis using a leaf extract fungi were tested battery clinical strains from cystic fibrosis, pneumonia burnt patients, some them with multidrug resistance. Both showed potent...
Abstract Antibiotic failure is one of the most worrisome threats to global health. Among new therapeutic efforts that are being explored, use bacteriophages (viruses kill bacteria), also known as ‘phages’, extensively studied a strategy target bacterial pathogens. However, main drawbacks phage therapy plethora defence mechanisms bacteria defend themselves against phages. This review aims summarize approaches evaluated overcome systems, including innovative applied: circumvention receptor...
Background Bacteriophage therapy is becoming part of mainstream Western medicine since antibiotics clinical use tend to fail. It involves applying lytic bacteriophages that self-replicate and induce cell lysis, thus killing their hosts. Nevertheless, bacterial promotes the selection resistant clones which sometimes may exhibit a decrease in virulence or antibiotic resistance. Methods In this work, we studied Pseudomonas aeruginosa phage φDCL-PA6 its variant φDCL-PA6α. Additionally,...