A5001805613- RNA Research and Splicing
- Plant Molecular Biology Research
- Amphibian and Reptile Biology
- Trypanosoma species research and implications
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Parasitic Infections and Diagnostics
- Force Microscopy Techniques and Applications
- Alzheimer's disease research and treatments
- Plant Reproductive Biology
- Plant and soil sciences
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Plant Diversity and Evolution
- Plant and animal studies
- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Sperm and Testicular Function
- Genomics and Phylogenetic Studies
- Research on Leishmaniasis Studies
- Endoplasmic Reticulum Stress and Disease
- Oral microbiology and periodontitis research
- Periodontal Regeneration and Treatments
- Reproductive biology and impacts on aquatic species
- Plant tissue culture and regeneration
Universidad Nacional Autónoma de México
2016-2025
Weatherford College
2024
Colegio de Postgraduados
2024
King Saud University
2023
Universidad Autónoma de la Ciudad de México
2016
Tel Aviv University
2002
Universidad Autónoma Metropolitana
1994
Instituto Nacional de Enfermedades Respiratorias
1994
Cold Spring Harbor Laboratory
1992-1993
State University of New York
1993
We have investigated the distribution of U3 snRNA and rRNA in HeLa cells normal rat kidney during interphase mitosis. snRNA, known to be involved pre-rRNA processing, was detected nucleoli coiled bodies interphase, whereas distributed throughout cytoplasm. By comparison, ribosomal protein S6 nucleoli, bodies, During nucleologenesis, observed newly forming late telophase but not prenucleolar (PNBs), PNBs. Similar findings those reported here for localization been previously small nuclear...
Endosymbiosis is a common phenomenon in nature, especially between bacteria and insects, whose typically unbalanced diets are usually complemented by their obligate endosymbionts. While much interest focus has been directed toward phloem-feeders like aphids mealybugs, blood-feeders such as the Lone star tick (Amblyomma americanum), Glossina flies, human body louse (Pediculus humanus corporis) depend on endosymbionts which complement B-vitamin-deficient diets, thus required for growth...
Abstract Pseudomonas aeruginosa is one of the most worrisome infectious bacteria due to its intrinsic and acquired resistance against several antibiotics recalcitrance infections; hence, development novel antimicrobials effective multidrug-resistant P. mandatory. In this work, silver nanoparticles obtained by green synthesis using a leaf extract fungi were tested battery clinical strains from cystic fibrosis, pneumonia burnt patients, some them with multidrug resistance. Both showed potent...
We report on a new, environment-friendly synthesis route to produce Fe3O4 magnetic nanoparticles (MNPs) from extracts of the plants Vanilla planifolia and Cinnamomun verum. These aqueous plant have double function reducing agents due their phenolic groups, also capping materials through –OH bonding over MNPs surface. The resulting average sizes ≈10–14 nm with core–shell Fe3O4–γFe2O3 structure surface oxidation driven by groups OH− covalent bonding. Saturation magnetization values MS = 70.84...
To date, no safe vaccine or antivirals for Zika virus (ZIKV) infection have been found. The pathogenesis of severe Zika, where host and viral factors participate, remains unclear. For the control it is important to understand how ZIKV interacts with different cells. Knowledge targeted cellular pathways which allow productively replicate and/or establish prolonged persistence contributes novel vaccines therapies. Monocytes endothelial vascular cells are main targets. During process, capable...
Zika fever is a reemerging arthropod-borne viral disease; however, virus (ZIKV) can be transmitted by other, non-vector means. Severe characterized neurological disorders, autoimmunity, or congenital syndrome. Monocytes are primary ZIKV targets in humans and, response to infection, release extracellular vesicles like exosomes. Exosomes mediate intercellular communication and involved the virus's ability circumvent immune response, promoting pathological processes. This study aimed evaluate...
Hemileia vastatrix coffee leaf rust reduces Mexican production by 51%. We aimed to analyze the size and distribution of H. urediniospores among plantations, as well morphological structures uredinium. In 2015, 65 samples with symptoms were collected from 17 cultivars grown at various altitudes (229-1649 m) under different environmental conditions in 14 regions four states. A total 30 spores per sample measured grouped using Ward centroid method, group was analyzed. Uredinia morphology...
Suicide is a significant public health problem, with increased rates in low- and middle-income countries such as Mexico; therefore, suicide prevention important. complex multifactorial phenomenon which biological social factors are involved. Several studies on the mechanisms of have analyzed proteome dorsolateral prefrontal cortex (DLPFC) people who died by suicide. The aim this work was to analyze protein expression profile DLPFC individuals comparison age-matched controls order gain...
ABSTRACT We have examined the cellular distribution of double-stranded RNA-activated protein kinase DAI in adenovirus 2 (Ad2) -infected and uninfected HeLa cells. In cells was found to be concentrated cytoplasm. addition, localized nucleoli diffusely distributed throughout nucleoplasm. Cells treated with α-interferon displayed a similar pattern for DAI. When RNA polymerase I activity inhibited by drug actinomycin D, segregated colocalize dense fibrillar region nucleoli. During mitosis,...
Splicing is an essential step in the processing of intron-containing pre-mRNAs prior to their transport out nucleus (for review, see Green 1991). During last few years, a significant number studies have addressed relationship pre-mRNA splicing nuclear structure Spector 1993). SnRNPs as well several non-snRNP factors been shown by immunofluorescence microscopy be localized speckled pattern that distributed various regions throughout nucleoplasm. Poly(A)+ RNA has colocalize with these speckles...
The mammalian cell nucleus is functionally compartmentalized into various substructures. Nuclear speckles, also known as interchromatin granule clusters, are enriched with SR splicing factors and implicated in gene expression. Here we report that nuclear speckle formation developmentally regulated; certain cases phosphorylated proteins absent from the instead localized at granular structures cytoplasm. To investigate how architecture formed, performed a phenotypic screen of HeLa cells...
Nucleoli are subcellular compartments where transcription and maturation of pre-ribosomal RNAs occur. While the ribosomal is common to all living cells, presence ultrastructure nucleoli has been only documented in eukaryotes. Asgard-Archaea, closest prokaryotic relatives eukaryotes, their near TACK-Archaea have homologs nucleolar proteins genome, but cellular organization both largely unexplored. Here we provide ultrastructural molecular evidence for putative nucleolus-like domains TACK...
The objectives of this investigation were a) to determine the best concentration chitosan reduce <em>Alternaria alternata</em> development in relation mycelial growth, germination and sporulation, b) observe through scanning electron microscopy effect level at morphological cellular c) evaluate efficiency compound control A. alternata mango cv. 'Tommy Atkins' fruits its on fruit ripening during storage.Results demonstrated that most effective was 1.0 %, because it reduced growth sporulation...
Enterococcus faecalis has been associated with root canal infections, while Streptococcus mutans a central role in the etiology of dental caries. One main reasons endodontic failure to presence E. and formation biofilms. S. inhabits oral cavity, specifically plaque, which is multispecies biofilm formed on hard surfaces tooth. The factor determining pathogenicity numerous bacteria. Natural antimicrobial peptides saliva protect against pathogenic bacteria aim this study was assess...
Chagas Disease is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi, affecting 6–8 million people, mainly in Latin America. The medical treatment based on two compounds, benznidazole and nifurtimox, with limited effectiveness that produce severe side effects; consequently, there an urgent need to develop new, safe, effective drugs. Amphotericin B most potent antimycotic known date. A21 derivative of this compound property binding ergosterol present cell...
Little is known about the molecular mechanisms that regulate cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as biological marker, we studied its expression and distribution in human periodontal tissues, ligament, alveolar bone, cementoblastoma-derived cells. In immunoreactivity to anti-CP was observed throughout cementoid phase of acellular cellular cementum,...
Insulin and ‘insulin‐like’ growth factors (IGFs) are known to regulate cell in eukaryotes by stimulating a signal transduction pathway that exerts translational control. Intermediate kinases of this pathway, target rapamycin (TOR) ribosomal protein S6 kinase (S6K), have been reported Arabidospsis thaliana Zea mays . However, upstream inducers downstream targets the not well plants. The objective work is inquire whether plant regulated similar insulin/IGF‐stimulated other metazoans. as...
Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from single Bovans White flock and exposed to L. monocytogenes. Artificially contaminated eggs divided into three different treatment groups: NEW, 2% citric acid solution (CAS), saline (SS). To evaluate bactericidal effect, Mexican norm for antimicrobial activity determination protocol performed. The observed effect compared those obtained CAS SS....
The increasing prevalence of conformational diseases, including Alzheimer's disease, type 2 Diabetes Mellitus and Cancer, poses a global challenge at many different levels. It has devastating effects on the sufferers as well tremendous economic impact families health system. In this work, we apply cross-functional approach that combines ideas, concepts technologies from several disciplines in order to study, silico vitro, role novel chemical chaperones family (NCHCHF) processes protein...
Human islet amyloid peptide (hIAPP1-37) aggregation is an early step in Diabetes Mellitus. We aimed to evaluate a family of pharmaco-chaperones act as modulators that provide dynamic interventions and the multi-target capacity (native state, cytotoxic oligomers, protofilaments fibrils hIAPP1-37) required meet treatment challenges diabetes. used cross-functional approach combines silico vitro biochemical biophysical methods study hIAPP1-37 aggregation-oligomerization process reveal novel...