A5071089651- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Biochemical and biochemical processes
- Horticultural and Viticultural Research
- Biosensors and Analytical Detection
- Microbial Metabolites in Food Biotechnology
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Phytochemical compounds biological activities
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Food composition and properties
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
Instituto de Investigación Biomédica de A Coruña
2016-2025
Complexo Hospitalario Universitario A Coruña
2016-2025
Universidade da Coruña
2018-2025
Fundación Seimc-Gesida
2020-2024
Ghent University
2019
Instituto de Biomedicina de Sevilla
2019
Hospital Universitario Virgen del Rocío
2019
Universidad de Sevilla
2019
Marqués de Valdecilla University Hospital
2019
Instituto de Investigación Marqués de Valdecilla
2019
The emergence of multidrug resistant (MDR) pathogenic bacteria is jeopardizing the value antimicrobials, which had previously changed course medical science. In this study, we identified endolysins ElyA1 and ElyA2 (GH108-PG3 family), present in genome bacteriophages Ab1051Φ Ab1052Φ, respectively. muralytic activity these against MDR clinical isolates (Acinetobacter baumannii, Pseudomonas aeruginosa Klebsiella pneumoniae) was tested using turbidity reduction assay. Minimal inhibitory...
Clinical case of a patient with Pseudomonas aeruginosa multidrug-resistant prosthetic vascular graft infection which was treated cocktail phages (PT07, 14/01, and PNM) in combination ceftazidime-avibactam (CZA). After the application phage treatment absence antimicrobial therapy, new P. bloodstream (BSI) septic residual limb metastasis occurred, now involving wild-type strain being susceptible to ß-lactams quinolones. strains were analyzed by microbiology whole genome sequencing techniques....
Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms seven clinical isolates A. baumannii. Microarray analysis one isolates, Ab1 (A. ST-2_clon_2010), previously cultured presence 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ...
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed search for new means of combatting imipenem-resistant persister strains K. by repurposing the anticancer drug mitomycin C as antimicrobial agent combining conventional imipenem with lytic phage vB_KpnM-VAC13. Several clinical isolates were characterized, isolate (harboring OXA-245 β-lactamase) a selected study....
The need for alternatives to antibiotic therapy due the emergence of multidrug resistant bacteria (MDR), such as nosocomial pathogen Acinetobacter baumannii, has led recovery phage therapy. In addition, phages can be combined in cocktails increase host range. this study, evolutionary mechanism adaptation was utilized order develop a adapted A. named Ab105-2phiΔCI404ad, from mutant lytic phage, Ab105-2phiΔCI, previously developed by our group. whole genome sequence Ab105-2phiΔCI404ad...
Abstract Antibiotic failure is one of the most worrisome threats to global health. Among new therapeutic efforts that are being explored, use bacteriophages (viruses kill bacteria), also known as ‘phages’, extensively studied a strategy target bacterial pathogens. However, main drawbacks phage therapy plethora defence mechanisms bacteria defend themselves against phages. This review aims summarize approaches evaluated overcome systems, including innovative applied: circumvention receptor...
Background Bacteriophage therapy is becoming part of mainstream Western medicine since antibiotics clinical use tend to fail. It involves applying lytic bacteriophages that self-replicate and induce cell lysis, thus killing their hosts. Nevertheless, bacterial promotes the selection resistant clones which sometimes may exhibit a decrease in virulence or antibiotic resistance. Methods In this work, we studied Pseudomonas aeruginosa phage φDCL-PA6 its variant φDCL-PA6α. Additionally,...
Phage therapy is an abandoned antimicrobial that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into lytic (Ab105-2phiΔCI) displayed activity against A. clinical strain Ab177_GEIH-2000 (isolated the GEIH-REIPI Spanish Multicenter Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585, and for which meropenem imipenem MICs of respectively, 32 µg/mL, 16 µg/mL were obtained). We observed vitro synergistic effect (reduction 4 log-7...
Abstract Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies demonstrated that TA play a key role in phage inhibition. The aim present study was to investigate PemIK (PemK/PemI) type II system inhibition by its intrinsic expression clinical strains Klebsiella pneumoniae carrying lncL plasmid, which harbours carbapenemase OXA-48 and PemK/PemI system. Furthermore, induced an IPTG-inducible plasmid reference strain K. ATCC10031...
To optimize phage therapy, we need to understand how bacteria evolve against attacks. One of the main problems therapy is appearance bacterial resistance variants. The use genomics track antimicrobial increasingly developed and used in clinical laboratories. For that reason, it important consider, an emerging future with detect avoid phage-resistant strains can be overcome by analysis metadata provided whole-genome sequencing. Here, identified genes associated 18 Acinetobacter baumannii...
Researchers, including microbiologists and infectious disease specialists, require more knowledge about the interactions between phages their bacterial hosts defense mechanisms. In this study, we analyzed molecular mechanisms of viral in infecting clinical isolates K. pneumoniae .
Abstract Phage tail-like bacteriocins (PTLBs) are large proteomic structures similar to the tail phages. These function in bacterial competition by making pores membrane of their competitors. The PTLBs identified Pseudomonas aeruginosa known as R-type and F-type pyocins, which have a narrow spectrum action. Their specificity is determined fiber closely related lipopolysaccharide type target competitor strain. In this study, genome sequences 32 clinical P. isolates were analysed investigate...
Klebsiella pneumoniae is the clinically most important species within genus and, as a result of continuous emergence multi-drug resistant (MDR) strains, cause severe nosocomial infections. The decline in effectiveness antibiotic treatments for infections caused by MDR bacteria has generated particular interest study bacteriophages. In this study, we characterized total 40 temperate bacteriophages (prophages) with genome range 11.454-84.199 kb, predicted from 16 carbapenemase-producing...
At the end of 2019, a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), caused pandemic that persists to date and has resulted in more than 6.2 million deaths. In last couple years, researchers have made great efforts develop diagnostic technique maintains high levels sensitivity specificity, since an accurate early diagnosis is required minimize prevalence SARS-CoV-2 infection. this context, CRISPR-Cas systems are proposed as promising tools for development...
ABSTRACT Pseudomonas aeruginosa is a bacterial pathogen that major cause of lung infections in cystic fibrosis (CF) and other patients. Isolates P. from CF patients commonly carry filamentous phages (Pf phages), which constitute family temperate known to be related biofilm production antibiotic sequestration. In this study, we identified 12 new Pf phage genomes collection clinical isolates Study the anti-phage defense systems revealed presence 89 such systems, eight were encoded genomes....
Introduction Antimicrobial resistance (AMR) is a major public health threat, driving the need for alternative treatments such as phage therapy. However, bacterial defense mechanisms, often regulated by quorum sensing (QS) network and encoded in genomic islands (GIs), can generate phage-resistant mutants. Understanding these mechanisms essential optimizing Methods This study analyzed 48 Klebsiella pneumoniae strains to identify pathogenicity (PAIs) containing anti-phage (APD) proteins. We...
ABSTRACT The widespread incidence of antimicrobial resistance has created renewed interest in the use alternative treatments such as phage therapy. Phages are viruses that infect bacteria and generally have a narrow host-range. Combining phages with antibiotics can prevent emergence bacterial resistance. aim present study was to develop therapy medical products (PTMPs) targeting clinical isolates carbapenem-producing Klebsiella pneumoniae belonging high-risk clone ST512. From collection 22...