A5054947609- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Oral microbiology and periodontitis research
- Dental Health and Care Utilization
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- 3D Printing in Biomedical Research
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Dental Implant Techniques and Outcomes
- Antibiotics Pharmacokinetics and Efficacy
- Bacillus and Francisella bacterial research
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
- Environmental Toxicology and Ecotoxicology
- HIV/AIDS oral health manifestations
- Marine Biology and Environmental Chemistry
- Photoacoustic and Ultrasonic Imaging
- Bacteriophages and microbial interactions
- Ocular Surface and Contact Lens
- Marine and coastal plant biology
- Plant Pathogenic Bacteria Studies
- Microplastics and Plastic Pollution
- Corneal Surgery and Treatments
Instituto de Investigación Biomédica de A Coruña
2024-2025
Complexo Hospitalario Universitario A Coruña
2024-2025
Universidade de Santiago de Compostela
2013-2024
Dentaid (Spain)
2024
Recent studies have revealed the presence of N-acyl-homoserine lactones (AHLs) quorum sensing (QS) signals in oral environment. Yet, their role biofilm development remains scarcely investigated. The use quenching (QQ) strategies targeting AHLs has been described as efficient for control pathogenic biofilms. Here, we evaluate a highly active AHL-targeting QQ enzyme, Aii20J, to modulate formation vitro. effect enzyme was studied vitro multispecies biofilms generated from samples taken healthy...
Acinetobacter baumannii presents a typical luxI/luxR quorum sensing (QS) system (abaI/abaR) but the acyl-homoserine lactone (AHL) signal profile and factors controlling production of QS signals in this species have not been determined yet. A very complex AHL was identified for A. ATCC17978 as well nosocomialis M2, only when cultivated under static conditions, suggesting that surface or cell-to-cell contact is involved activation genes. The analysis baumanni clinical isolates revealed...
Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms seven clinical isolates A. baumannii. Microarray analysis one isolates, Ab1 (A. ST-2_clon_2010), previously cultured presence 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ...
The important nosocomial pathogen Acinetobacter baumannii presents a quorum sensing (QS) system (abaI/abaR) mediated by acyl-homoserine-lactones (AHLs) and several quenching (QQ) enzymes. However, the role of this complex network in control expression virulence-related phenotypes such as surface-associated motility biofilm formation are not clear. Therefore, effect mutation AHL synthase AbaI, exogenous addition QQ enzyme Aii20J on A. ATCC® 17978TM was studied detail. multidrug-resistant...
Acyl homoserine lactones (AHLs), the quorum sensing (QS) signals produced by Gram-negative bacteria, are currently considered to play a minor role in development of oral biofilm since their production pathogens has not been ascertained thus far. However, we report presence AHLs different samples and pathogen Porphyromonas gingivalis. The importance is further supported very high prevalence AHL-degradation capability, up 60%, among bacteria isolated from dental plaque saliva samples....
Contact lenses (CLs) are prone to biofilm formation, which may cause severe ocular infections. Since the use of antibiotics is associated with resistance concerns, here, two alternative strategies were evaluated endow CLs antibiofilm features: copolymerization antifouling monomer 2-methacryloyloxyethyl phosphorylcholine (MPC) and loading antioxidant resveratrol known antibacterial activity. MPC has, so far, been used increase water retention on CL surface (Proclear® 1 day CLs). Both...
ABSTRACT Carbapenemase OXA-48 and its variants pose a serious threat to the development of effective treatments for bacterial infections. OXA-48-producing Enterobacterales are most prevalent carbapenemase-producing bacteria in large parts world. Although these exhibit low-level carbapenem resistance vitro , infections they cause challenging treat with conventional therapies, owing their spread complex detection clinical settings. However, numerous β-lactamase inhibitors (BLIs) currently...
There is increasing evidence being accumulated regarding the importance of
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 108:217-225 (2014) - DOI: https://doi.org/10.3354/dao02697 In vitro quenching fish pathogen Edwardsiella tarda AHL production using marine bacterium Tenacibaculum sp. strain 20J cell extracts Manuel Romero, Andrea Muras, Celia Mayer, Noemí Buján, Beatriz Magariños, Ana Otero* Departamento de Microbiología y...
Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent biofilm formation by Streptococcus mutans, pathogen involved in tooth decay.Objective: To obtain inhibition of S. mutans extracts obtained from marine bacterium Tenacibaculum sp. 20J interfering with system.Design: The inhibitory activity was tested biosensors Vibrio harveyi BB170 and JMH597. ATCC25175 monitored using impedance real-time measurements xCELLigence system®, confocal laser...
Dental plaque is omnipresent in healthy oral cavities and part of our commensal microbial colonization. At the same time, dental cause most common human diseases, caries gum disease. consists billions microbes attached to surface your teeth. Communication among these pivotal for development complex communities yet poorly studied plaque. In present study, we show that a specific communication molecule induces changes within community related This finding suggests interfering with may...
Quorum sensing (QS) is a mechanism dependent on bacterial density. This coordinated process mediated by the synthesis and secretion of signal molecules, called autoinducers (AIs). N-acyl-homoserine lactones (AHLs) are most common AIs that used Gram-negative bacteria involved in biofilm formation. Quenching (QQ) interference QS producing hydrolyzing enzymes, among other strategies. The main objective present study was to identify QQ strains from MBR wastewater treatment plants. A total 99...
While increasing evidence supports a key role of bacteria in coral larvae settlement and development, the relative importance environmentally-acquired versus vertically-transferred bacterial population is not clear. Here we have attempted to elucidate post-brooding-acquired on development planula-larvae octocoral Rhytisma f. fulvum, an vitro cultivation system employing different types filtered (FSW) autoclaved (ASW) seawater with addition native bacteria. A good was obtained polystyrene...
The rise in multidrug-resistant bacteria challenges clinical microbiology. Tigecycline, eravacycline, and omadacycline show promise against carbapenem-resistant Enterobacterales Acinetobacter baumannii. This study evaluates their activity resistance mechanisms.
Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control biofilm formation, key process in biofouling development. Appropriate vitro biofilm-forming bacteria models are needed establish screening methods for innovative anti-biofilm and anti-microfouling compounds. Four marine strains, two Pseudoalteromonas spp. Vibrio spp., were selected studied regard their capacity sensitivity (QS) inhibitors. Biofilm experiments performed...
The current study aimed to screen Bacillus strains with wide-spectrum quorum quenching (QQ) activity against N-acyl-l-homoserine lactones (AHLs), helpful in controlling virulence traits Gram-negatives, including biofilm formation and also anti-biofilm Gram-positives.A total of 94 halotolerant isolated from soil salt-lake sediment samples Algeria were examined for the presence QQ AHLs, aiiA gene, encoding an AHL lactonase enzyme typical spp., antimicrobial activities Pseudomonas aeruginosa...
There is an increasing interest in developing innovative coatings and testing natural products with anti-fouling activity to substitute current highly toxic biocides that have a harmful impact on marine organisms. Bacillus licheniformis species shown different anti-biofilm activities vitro , but so far, its efficacy field trials has not been tested. For this purpose, the capacity of extracts B. NCTC 10341 T prevent micro macro-fouling was first tested . The methanol cell extract (MCE)...
<title>Abstract</title> Objective This study aimed to evaluate the impact of 0.05% and 0.12% chlorhexidine (CHX) mouthwashes on peri-implant pocket microbiota patients with peri-implantitis. Materials methods We analyzed microbial composition lesions in 22 before after a 15-day regimen thrice-daily use two commercial CHX containing (n = 11) 11). Microbial samples were collected using paper points taxonomic was determined through sequencing 16S rRNA gene amplicons Illumina MiSeq. Results...
The microbial compositions from concurrent peri-implant and periodontal lesions were compared, since the results reported in literature on etiological relationship between these oral pathologies are contradictory. Microbial nine patients evaluated using Illumina MiSeq sequencing of 16S rRNA gene amplicons Principal Components Analysis. Comparisons use curettes or paper points as collection methods bacterial composition both performed. Paper allowed recovery a higher number genera. A...