A5033681783- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 detection and testing
- Vibrio bacteria research studies
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Oral microbiology and periodontitis research
- Pharmaceutical and Antibiotic Environmental Impacts
- Gut microbiota and health
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Poxvirus research and outbreaks
- Escherichia coli research studies
- Plant Virus Research Studies
- Antibiotics Pharmacokinetics and Efficacy
- Genomics and Phylogenetic Studies
- COVID-19 diagnosis using AI
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Salivary Gland Disorders and Functions
- Antimicrobial Peptides and Activities
- Colorectal Cancer Screening and Detection
- Viral Infections and Outbreaks Research
- Legionella and Acanthamoeba research
Complexo Hospitalario Universitario A Coruña
2010-2024
Instituto de Investigación Biomédica de A Coruña
2011-2024
Centro de Investigación Biomédica en Red
2022-2024
Universidade da Coruña
2020-2024
Instituto de Salud Carlos III
2023-2024
Biomedical Research Institute
2020
Acinetobacter baumannii has emerged as a dangerous opportunistic pathogen, with many strains able to form biofilms and thus cause persistent infections. The aim of the present study was use high-throughput sequencing techniques establish complete transcriptome profiles planktonic (free-living) sessile (biofilm) forms A. ATCC 17978 thereby identify differences in their gene expression patterns. Collections mRNA from (both exponential stationary phase cultures) cells were sequenced. Six...
Multidrug efflux pumps have emerged as important mechanisms of antimicrobial resistance in bacterial pathogens. In order to cause infection, pathogenic bacteria require avoid the effects host-produced compounds, and express may accomplish this task. study, we evaluated effect inactivation AcrAB-TolC on resistance, fitness, virulence Enterobacter cloacae, an opportunistic pathogen usually involved nosocomial infections. Two different clinical isolates E. cloacae were used, EcDC64 (multidrug...
RecA is the major enzyme involved in homologous recombination and plays a central role SOS mutagenesis. In Acinetobacter spp., including baumannii , multidrug-resistant bacterium responsible for nosocomial infections worldwide, DNA repair responses differ many ways from those of other bacterial species. this work, function A. was examined by constructing recA mutant. Alteration single gene had pleiotropic effect, showing involvement damage consequently cellular protection against stresses...
The quantification of the SARS-CoV-2 RNA load in wastewater has emerged as a useful tool to monitor COVID–19 outbreaks community. This approach was implemented metropolitan area A Coruña (NW Spain), where from treatment plant analyzed track epidemic dynamics population 369,098 inhabitants. Viral detected and epidemiological data health system served main sources for statistical models developing. Regression described here allowed us estimate number infected people (R2 = 0.9), including...
To evaluate the activity of cefiderocol, imipenem/relebactam, cefepime/taniborbactam and cefepime/zidebactam against a clinical laboratory collection ceftolozane/tazobactam- ceftazidime/avibactam-resistant Pseudomonas aeruginosa β-lactamase mutants.The cefepime/taniborbactam, comparators was evaluated 30 molecularly characterized and/or P. isolates from patients previously treated with cephalosporins. how different β-lactamases in affected resistance to these agents, copy each blaPDC,...
The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral gut microbiomes patients with CRC, as well impaired vascular barrier function. In present study, saliva, crevicular fluid, feces, non‐neoplastic tumor intestinal tissue samples 93 30 healthy individuals without digestive disorders (non‐CRC) were analyzed by...
Acinetobacter baumannii is a multidrug-resistant bacterium responsible for nosocomial infections in hospitals worldwide. Study of mutant phenotypes fundamental understanding gene function. The methodologies developed to inactivate A. genes are complicated and time-consuming; sometimes result unstable mutants, do not enable construction double (or more) knockout strains baumannii.We describe here rapid simple method obtaining mutants by replacement via crossover recombination, use PCR product...
The important nosocomial pathogen Acinetobacter baumannii presents a quorum sensing (QS) system (abaI/abaR) mediated by acyl-homoserine-lactones (AHLs) and several quenching (QQ) enzymes. However, the role of this complex network in control expression virulence-related phenotypes such as surface-associated motility biofilm formation are not clear. Therefore, effect mutation AHL synthase AbaI, exogenous addition QQ enzyme Aii20J on A. ATCC® 17978TM was studied detail. multidrug-resistant...
Understanding microbial populations in hospital environments is crucial for improving human health. Hospital-acquired infections are an increasing problem intensive care units (ICU). In this work we present exploration of bacterial diversity at inanimate surfaces the ICU wards University Hospital A Coruña (Spain), as example confined environment subjected to selective pressure, taking entrance hall hospital, open and crowded environment, reference. Surface swab samples were collected from...
Acinetobacter baumannii is a hospital-acquired pathogen that shows an extraordinary capacity to stay in the hospital environment. Adherence of bacteria eukaryotic cells or abiotic surfaces first step for establishing infection. The A. strain AbH12O-A2 showed exceptional ability adhere A549 epithelial cells. AbFhaB/FhaC 2-partner secretion (TPS) system involved adhesion was discovered after screening recently determined genome (CP009534.1). AbFhaB large exoprotein which transport bacterial...
Acinetobacter baumannii is a nosocomial pathogen that has considerable ability to survive in the hospital environment partly due its capacity form biofilms. The first step process of establishing an infection adherence bacteria target cells. Chaperone-usher pili assembly systems are involved pilus biogenesis pathways play important role adhesion host cells and tissues as well medically relevant surfaces. After screening collection strains, biofilm hyper-producing A. strain (MAR002) was...
ABSTRACT The quantification of the SARS-CoV-2 load in wastewater has emerged as a useful method to monitor COVID-19 outbreaks community. This approach was implemented metropolitan area A Coruña (NW Spain), where from treatment plant Bens analyzed track epidemic’s dynamic population 369,098 inhabitants. We developed statistical regression models that allowed us estimate number infected people viral detected with reliability close 90%. is first wastewater-based epidemiological model could...
β-Lactamases and penicillin-binding proteins (PBPs) have evolved from a common ancestor. are enzymes that degrade β-lactam antibiotics, whereas PBPs involved in the synthesis processing of peptidoglycan, which forms an elastic network bacterial cell wall. This study analyzed interaction between β-lactamases peptidoglycan impact on fitness biofilm production. A representative set all classes was cloned expression vector pBGS18 under control CTX-M promoter expressed Escherichia coli MG1655....
Abstract Objectives To describe and characterize the emergence of resistance to ceftolozane/tazobactam, ceftazidime/avibactam imipenem/relebactam in a patient receiving treatment for an MDR Pseudomonas aeruginosa CNS infection. Methods One baseline (PA1) two post-exposure (PA2 PA3) isolates obtained before during nosocomial P. meningoventriculitis were evaluated. MICs determined by broth microdilution. Mutational changes investigated through WGS. The impact on β-lactam mutations blaPDC mexR...
Genetic and functional studies showed that some components of the Acinetobacter baumannii ATCC 17978 A1S_0112-A1S_0119 gene cluster are critical for biofilm biogenesis surface motility. Recently, our group has shown A1S_0114 was involved in formation, a process related with pathogenesis. Confirming previous results, microscopy images revealed Δ0114 derivative lacking this unable to form mature structure. Therefore, other bacterial phenotypes were analyzed determine role pathogenicity A....
Significance Emergence of the pathogen Klebsiella pneumoniae (particularly carbapenem-resistant strains) is considered a dire threat to public health. Resistance and virulence determinants may favor emergence untreatable infections. Understanding mechanisms involved in pathogenesis epidemicity K. essential for managing outbreaks developing treatments. Here we identify CUP system (Kpi) infer epidemiology Kpi + Europe. We demonstrate direct link between presence K . persistence hospital...
Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients 30 healthy controls (non‐CRC) were collected for microbiome analysis. Saliva (28 non‐CRC 94 CRC), feces (30 97 subgingival fluid (20 tumor tissue CRC) used 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis the abundance, performed with ANCOM‐BC package, adjusting P ‐values by Holm‐Bonferroni method, revealed that Parvimonas was significantly over‐represented in CRC ( ‐value...
Wastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from WWTP A Coruña (NW Spain) April 2020 February 2022 evaluate presence Gram-negative harboring carbapenemase genes. Bacteria isolated were classified and their antimicrobial profiles determined. total, 252 carrying various genes described. Whole-genome sequencing was conducted on 55 selected...
Acinetobacter baumannii is a major cause of antibiotic-resistant nosocomial infections worldwide. In this study, several rifampin-resistant spontaneous mutants obtained from the A. ATCC 17978 strain that differed in their point mutations rpoB gene, encoding β-subunit RNA polymerase, were isolated. All harboring amino acid substitutions position 522 or 540 RpoB protein impaired surface-associated motility and had attenuated virulence fertility model Caenorhabditis elegans The transcriptional...
Acinetobacter baumannii is a multidrug-resistant pathogen that represents serious threat to global health. A. possesses wide range of virulence factors contribute the bacterial pathogenicity. Among them, siderophore acinetobactin one most important, being essential for development infection. In this study we performed an in-depth analysis cluster in strain ATCC 17978. For purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical performed. Individual...
Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect...
Abstract The dynamics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) transmission are influenced by a variety factors, including social restrictions and the emergence distinct variants. In this study, we delve into origins dissemination Alpha, Delta, Omicron‐BA.1 variants concern in Galicia, northwest Spain. For this, leveraged genomic data collected EPICOVIGAL Consortium from GISAID database, along with mobility information other Spanish regions foreign countries. Our...
The new metallo-β-lactamase VIM-13 has been recently characterized. In comparison with the VIM-1 enzyme, showed 19 amino acid differences, 2 of which were located in active site centre. main objective present study was to assess whether differences between and β-lactamases site, at His224Leu Ser228Arg, are necessary sufficient explain microbiological biochemical two enzymes. Single mutants (Leu224His) (Arg228Ser) double mutant (Leu224His, Arg228Ser) created by site-directed mutagenesis...