A5012261161- Gut microbiota and health
- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- Malaria Research and Control
- Diet and metabolism studies
- Aquaculture disease management and microbiota
- Bacterial Identification and Susceptibility Testing
- COVID-19 epidemiological studies
- Pneumonia and Respiratory Infections
- Drug Transport and Resistance Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Respiratory and Cough-Related Research
- Gastrointestinal motility and disorders
- Infective Endocarditis Diagnosis and Management
- Infection Control and Ventilation
- COVID-19 and Mental Health
- Oral Health Pathology and Treatment
- Hemoglobinopathies and Related Disorders
- Probiotics and Fermented Foods
- HIV/AIDS oral health manifestations
- Global Maternal and Child Health
- Cancer, Stress, Anesthesia, and Immune Response
- Streptococcal Infections and Treatments
- Colorectal Cancer Screening and Detection
- Cancer Research and Treatments
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2019-2025
Zero to Three
2023
Universitat de Barcelona
2022
Foundation Center
2022
Barcelona Institute for Global Health
2022
Institut de Recerca Sant Joan de Déu
2020
Abstract Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study composition human microbiota. Unfortunately, short reads are unable differentiate between highly similar species. Considering that species from same genus can be associated health or disease it important identify them at lowest possible taxonomic rank. Third-generation sequencing platforms such as PacBio SMRT, increase read lengths allowing sequence whole maximum...
The salivary glands actively concentrate plasma nitrate, leading to high nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal. Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on oral microbiota have not been clarified. To test this, saliva 12 healthy donors was collected grow vitro biofilms with without 6.5 mM nitrate. Samples were taken at 5 h (most reduced) 9 (all biofilm formation for...
The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral gut microbiomes patients with CRC, as well impaired vascular barrier function. In present study, saliva, crevicular fluid, feces, non‐neoplastic tumor intestinal tissue samples 93 30 healthy individuals without digestive disorders (non‐CRC) were analyzed by...
Colorectal cancer (CRC) and periodontitis have recently been related due to the higher incidence of CRC in periodontal patients involvement pathogens carcinogenesis, suggesting that leakage from oral cavity gut occurs. However, magnitude this pass-through healthy individuals is controversial, effect could play it understudied. To evaluate rate bacterial gut, we analyzed microbial composition saliva, subgingival plaque, fecal samples without gastrointestinal disorders, including 20 controls,...
<title>Abstract</title> Background Colorectal cancer (CRC) is the second leading cause of cancer-related deaths, accounting for more than 900,000 deaths a year worldwide. Microbial dysbiosis, including presence oral bacteria in gut has been linked to CRC. Some mechanisms by which specific microorganisms potentially drive tumorigenesis have described, but there lack studies elucidating whole microbiota activity tumor and their implication development disease. Here, metatrascriptome control...
Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- multiple-species oral biofilms real time through impedance technology, which elucidates the kinetics of biofilm formation 96-well plates, without requirement for any further manipulation. Design Results: Using this system, Streptococcus mutans appear be sugar-dependent highly resistant amoxicilin, an antibiotic pathogen is sensitive planktonic state. Saliva, tongue dental plaque samples...
Abstract Background Susceptibility of children and adults to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection persistence antibody response the virus after resolution remain poorly understood, despite their significant public health implications. Methods A prospective cross-sectional seroprevalence study with volunteer families that included at least 1 first-reported adult case positive by SARS-CoV-2 polymerase chain reaction (PCR) child aged &lt;15 years living in...
Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients 30 healthy controls (non‐CRC) were collected for microbiome analysis. Saliva (28 non‐CRC 94 CRC), feces (30 97 subgingival fluid (20 tumor tissue CRC) used 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis the abundance, performed with ANCOM‐BC package, adjusting P ‐values by Holm‐Bonferroni method, revealed that Parvimonas was significantly over‐represented in CRC ( ‐value...
Abstract Background Low-density Plasmodium falciparum infections prevail in low transmission settings, where immunity is expected to be minimal, suggesting an immune-independent effect on parasite densities. We aimed describe densities pregnancy, and determine how gravidity antibody-mediated affect these, during a period of declining malaria southern Mozambique. Methods documented P. at first antenatal care visits ( n = 6471) between November 2016 October 2019 Ilha Josina (high-to-moderate...
Abstract Background. Sequencing variable regions of the 16S rRNA gene (≃300bp) with Illumina technology is commonly used to study composition human microbiota. Unfortunately, short reads are unable differentiate between highly similar species. Considering that species from same genus can be associated health or disease it important identify them at lowest possible taxonomic rank. Third-generation sequencing platforms such as PacBio SMRT, increase read lengths allowing sequence whole maximum...
Abstract Background : The carcinogenesis of colorectal cancer (CRC) is a multifactorial process involving both environmental and host factors, such as human genetics or the gut microbiome, which in CRC patients appears to be enriched oral microorganisms. aim this work was investigate presence activity Parvimonas micra patients. To do that, samples collected from subgingival sulcus neoplastic lesions were used for culturomics. Then, different body locations (saliva, gingival crevicular fluid,...
Biofilm formation by the pathobiont Haemophilus influenzae is associated with human nasopharynx colonization, otitis media in children, and chronic respiratory infections adults suffering from diseases such as obstructive pulmonary disease (COPD). β-lactam quinolone antibiotics are commonly used to treat these infections. However, considering resistance of biofilm-resident bacteria antibiotic-mediated killing, use may be insufficient require being replaced or complemented novel strategies....
Abstract Background Ulcerative colitis (UC) is characterized by a diffuse, continuous, and chronic inflammation of mucosa submucosa layers in the colon whose etiology still unknown. Intestinal microbiota dysbiosis alterations metabolomic profile have been reported mucosal biopsies from Inflammatory Bowel Disease (IBD) patients. We aim to characterize composition colonic resections UC Methods Colonic (n=18) non-IBD (n=20) patients were obtained. Microbiota identification, classification was...
Abstract Background Crohn’s Disease (CD) is a subtype of IBD characterized by chronic transmural inflammation the gastrointestinal tract associated with several complications being intestinal fibrosis most frequent. CD patients present microbiota dysbiosis and altered metabolomic profiles. GPCRs constitute family receptors which could be involved in inflammatory fibrotic processes to CD. We aim characterize composition, tissue profile metabolite-sensing expression ileal resections from...
Abstract Background Metabolomics is a recent technique that has bounced into Inflammatory Bowel Diseases (IBD) due to its capacity elucidate specific metabolites involved in the pathology and changes metabolomic profile have been detected urine, blood or feces from UC patients. G-protein coupled receptors (GPCRs) recently identified as promising pharmacological targets. We aim characterize metabolite-sensing GPCRs expression colonic resections Methods Colonic (n=18) non-IBD (n=20) patients...
Background: Low-density Plasmodium falciparum infections prevail in low transmission settings, where immunity is expected to be minimal. We aimed describe parasite densities pregnancy during declining malaria southern Mozambique. Methods: documented P. at first antenatal care visits (n=6471) between November 2016 and October 2019 Ilha Josina (moderate area), Manhiça (low Magude (pre-elimination area). Two-way interactions mixed-effects regression models were used assess gravidity-dependent...