A5024666798- Antibiotic Resistance in Bacteria
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Biosensors and Analytical Detection
- Oral microbiology and periodontitis research
- Antibiotics Pharmacokinetics and Efficacy
- Vibrio bacteria research studies
- COVID-19 Clinical Research Studies
- Colorectal Cancer Screening and Detection
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Aquaculture disease management and microbiota
- COVID-19 diagnosis using AI
- Antimicrobial Peptides and Activities
- Aquatic Invertebrate Ecology and Behavior
- Salivary Gland Disorders and Functions
- Metabolomics and Mass Spectrometry Studies
- Antibiotic Use and Resistance
- Mycobacterium research and diagnosis
- Essential Oils and Antimicrobial Activity
- Healthcare and Environmental Waste Management
- COVID-19 epidemiological studies
Instituto de Investigación Biomédica de A Coruña
2019-2025
Centro de Investigación Biomédica en Red
2022-2025
Complexo Hospitalario Universitario A Coruña
2019-2025
Universidade da Coruña
2019-2025
Instituto de Salud Carlos III
2023-2024
Zero to Three
2023
The quantification of the SARS-CoV-2 RNA load in wastewater has emerged as a useful tool to monitor COVID–19 outbreaks community. This approach was implemented metropolitan area A Coruña (NW Spain), where from treatment plant analyzed track epidemic dynamics population 369,098 inhabitants. Viral detected and epidemiological data health system served main sources for statistical models developing. Regression described here allowed us estimate number infected people (R2 = 0.9), including...
The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral gut microbiomes patients with CRC, as well impaired vascular barrier function. In present study, saliva, crevicular fluid, feces, non‐neoplastic tumor intestinal tissue samples 93 30 healthy individuals without digestive disorders (non‐CRC) were analyzed by...
Imipenem/relebactam is a novel carbapenem/β-lactamase inhibitor combination, developed to act against carbapenemase-producing Enterobacterales (CPE).To assess the in vitro activity of imipenem/relebactam Spanish nationwide collection CPE by testing susceptibility these isolates 16 widely used antimicrobials and determine underlying β-lactam resistance mechanisms involved molecular epidemiology carbapenemases Spain.Clinical (n = 401) collected for 2 months from 24 hospitals Spain were tested....
ABSTRACT The quantification of the SARS-CoV-2 load in wastewater has emerged as a useful method to monitor COVID-19 outbreaks community. This approach was implemented metropolitan area A Coruña (NW Spain), where from treatment plant Bens analyzed track epidemic’s dynamic population 369,098 inhabitants. We developed statistical regression models that allowed us estimate number infected people viral detected with reliability close 90%. is first wastewater-based epidemiological model could...
Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect...
Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients 30 healthy controls (non‐CRC) were collected for microbiome analysis. Saliva (28 non‐CRC 94 CRC), feces (30 97 subgingival fluid (20 tumor tissue CRC) used 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis the abundance, performed with ANCOM‐BC package, adjusting P ‐values by Holm‐Bonferroni method, revealed that Parvimonas was significantly over‐represented in CRC ( ‐value...
Wastewater treatment plants (WWTPs) are recognized as important niches of antibiotic-resistant bacteria that can be easily spread to the environment. In this study, we collected wastewater samples from WWTP A Coruña (NW Spain) April 2020 February 2022 evaluate presence Gram-negative harboring carbapenemase genes. Bacteria isolated were classified and their antimicrobial profiles determined. total, 252 carrying various genes described. Whole-genome sequencing was conducted on 55 selected...
LpxB is an enzyme involved in the biosynthesis pathway of lipid A, a component LPS.To evaluate lpxB gene Acinetobacter baumannii as potential therapeutic target and to propose antisense agents such peptide nucleic acids (PNAs) tool combat bacterial infection, either alone or combination with known antimicrobial therapies.RNA-seq analysis A. ATCC 17978 strain murine pneumonia model was performed study vivo expression lpxB. Protein studied presence absence anti-lpxB (KFF)3K-PNA (pPNA)....
Acinetobacter baumannii is a multidrug-resistant pathogen that represents serious threat to global health. A. possesses wide range of virulence factors contribute the bacterial pathogenicity. Among them, siderophore acinetobactin one most important, being essential for development infection. In this study we performed an in-depth analysis cluster in strain ATCC 17978. For purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical performed. Individual...
Abstract Objectives To evaluate the effect of coconut oil on oral bacteriome and inflammatory response in patients with periodontitis by integrating next-generation sequencing analyses pathogenic bacterial shifts quantification markers, thereby assessing its potential as a natural adjunct to standard nonsurgical periodontal therapy. Materials methods A triple-blind clinical trial was conducted 30 participants diagnosed periodontitis, randomized into 3 groups: (1) oil, (2) chlorhexidine (3)...
<title>Abstract</title> Background Colorectal cancer (CRC) is the second leading cause of cancer-related deaths, accounting for more than 900,000 deaths a year worldwide. Microbial dysbiosis, including presence oral bacteria in gut has been linked to CRC. Some mechanisms by which specific microorganisms potentially drive tumorigenesis have described, but there lack studies elucidating whole microbiota activity tumor and their implication development disease. Here, metatrascriptome control...
Abstract Background Infections caused by multidrug-resistant pathogens such as Acinetobacter baumannii constitute a major health problem worldwide. In this study we present global in vivo transcriptomic analysis of A. isolated from the lungs mice with pneumonia infection. Methods Mice were infected ATCC 17978 and AbH12O-A2 strains total bacterial RNA analyzed sequencing. Lists differentially expressed genes obtained 14 them selected for gene deletion further analysis. Results Transcriptomic...
Pharmaceutical active compounds (PhACs) are emerging contaminants that pose a growing concern due to their ubiquitous presence and harmful impact on aquatic ecosystems. Among PhACs, the anti-inflammatory ibuprofen (IBU) antibiotic oxytetracycline (OTC) two of most used whose has been reported in different environments worldwide. However, there is still scarce information about cellular molecular alterations provoked by IBU OTC photosynthetic microorganisms as microalgae, even more if we...
Acinetobacter baumannii is currently considered one of the most problematic nosocomial microorganisms. In present work hisF gene from ATCC 17978 strain and AbH12O-A2 clinical isolate A. was found over-expressed during course murine pneumonia infections. The study demonstrated that mutant lacking induces a sub-lethal infection in mice, while complemented increased its virulence. This histidine auxotroph showed an increase on IL-6 secretion leukocytes recruitment Furthermore, data revealed...
Abstract The dynamics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) transmission are influenced by a variety factors, including social restrictions and the emergence distinct variants. In this study, we delve into origins dissemination Alpha, Delta, Omicron‐BA.1 variants concern in Galicia, northwest Spain. For this, leveraged genomic data collected EPICOVIGAL Consortium from GISAID database, along with mobility information other Spanish regions foreign countries. Our...
The discovery of new antibiotics that are effective against Acinetobacter baumannii and Enterobacteralesis a research priority. Several essential oils (EOs) have displayed some antimicrobial activity could potentially act as antibiotic adjuvants. Research in this area aims to develop therapeutic alternatives treat infections caused by these pathogens. MICs different EOs were determined A. Klebsiella pneumoniae. Combined disk diffusion tests checkerboard assays used study the synergy between...
The carbapenem-hydrolyzing class D β-lactamases (CHDLs) are the main mechanism of carbapenem resistance in Acinetobacter baumannii . CHDLs not effectively inactivated by clinically available β-lactam-type inhibitors. We have previously described vitro efficacy inhibitor LN-1-255 combination with carbapenems.
Abstract The present work describes a statistical model to account for sequencing information of SARS-CoV-2 variants in wastewater samples. expresses the joint probability distribution number genomic reads corresponding mutations and non-mutations every locus terms variant proportions mutation within variant. Since can be estimated using GISAID data, only unknown parameters are proportions. These maximum likelihood. method is applied monitor evolution data coming from samples collected A...
Abstract Background : The carcinogenesis of colorectal cancer (CRC) is a multifactorial process involving both environmental and host factors, such as human genetics or the gut microbiome, which in CRC patients appears to be enriched oral microorganisms. aim this work was investigate presence activity Parvimonas micra patients. To do that, samples collected from subgingival sulcus neoplastic lesions were used for culturomics. Then, different body locations (saliva, gingival crevicular fluid,...
Wastewater-based epidemiology (WBE) has become an effective tool in the surveillance of infectious diseases such as COVID-19. In this work, we performed a brief study monitoring SARS-CoV-2 viral load wastewater from six nursing homes located metropolitan area A Coruña (Spain) between December 2020 and March 2021. The main objective was to detect outbreaks among residents efficacy vaccination campaign. (RNA copies per L wastewater) determined by reverse-transcription quantitative PCR...
The dynamics of SARS-CoV-2 transmission are influenced by a variety factors, including social restrictions and the emergence distinct variants. In this study, we delve into origins dissemination Alpha, Delta, Omicron variants concern in Galicia, northwest Spain. For this, leveraged genomic data collected EPICOVIGAL Consortium from GISAID database, along with mobility information other Spanish regions foreign countries. Our analysis indicates that initial introductions during Alpha phase were...