A5030813267- Extracellular vesicles in disease
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Corneal Surgery and Treatments
- RNA modifications and cancer
- Probiotics and Fermented Foods
- Aortic aneurysm repair treatments
- Bacterial biofilms and quorum sensing
- Ocular Oncology and Treatments
- T-cell and B-cell Immunology
- Retinal Development and Disorders
- Oral microbiology and periodontitis research
- CAR-T cell therapy research
Instituto de Investigación Sanitaria de Santiago
2020-2025
Servicio Gallego de Salud
2020-2025
Universidade de Santiago de Compostela
2018
Extracellular vesicles (EVs) have recently emerged as a relevant way of cell to communication, and its analysis has become an indirect approach assess the cell/tissue origin status. However, knowledge about their nature role on metabolic diseases is still very scarce. We established insulin resistant (IR) two lipid (palmitic/oleic) hypertrophied adipocyte models isolate EVs perform protein cargo qualitative quantitative Sequential Window Acquisition All Theoretical Mass Spectra (SWATH) by...
Brown adipose tissue (BAT) is a key target for the development of new therapies against obesity due to its role in promoting energy expenditure; BAT secretory capacity emerging as an important contributor systemic effects, which extracellular vesicles (EVs) (i.e., batosomes) might be protagonists. EVs have emerged relevant cellular communication system and carriers disease biomarkers. Therefore, characterization protein cargo batosomes reveal their potential biomarkers metabolic activity...
Extracellular vesicles (EVs) represent a sophisticated mechanism of intercellular communication that is implicated in health and disease. Specifically, the role EVs metabolic regulation their implications pathologies, such as obesity its comorbidities, remain unclear. were isolated through serial ultracentrifugation from murine adipocytes treated with palmitate or oleic acid, whole visceral subcutaneous adipose tissue (obesesomes) bariatric surgery obese donors, human hepatocytes under...
Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent biofilm formation by Streptococcus mutans, pathogen involved in tooth decay.Objective: To obtain inhibition of S. mutans extracts obtained from marine bacterium Tenacibaculum sp. 20J interfering with system.Design: The inhibitory activity was tested biosensors Vibrio harveyi BB170 and JMH597. ATCC25175 monitored using impedance real-time measurements xCELLigence system®, confocal laser...
ALK rearrangements are present in 5% of nonsmall cell lung cancer (NSCLC) tumors and identify patients who can benefit from inhibitors. fusions testing using liquid biopsies, although challenging, expand the therapeutic options for ALK-positive NSCLC considerably. RNA inside extracellular vesicles (EVs) is protected RNases other environmental factors, constituting a promising source noninvasive fusion transcript detection.EVs H3122 H2228 lines, harboring EML4-ALK variant 1 (E13; A20) 3...
Metastatic melanoma presents, in many cases, oncogenic mutations BRAF, a MAPK involved proliferation of tumour cells. BRAF inhibitors, used as therapy patients with these mutations, often lead to resistance and, thus, the use MEK inhibitors was introduced clinics. BRAFi/MEKi, combination that has modestly increased overall survival patients, been proven differentially affect immune ligands, such NKG2D-ligands, drug-sensitive vs. drug-resistant However, fact NKG2D-ligands can be released...
Introduction: PARK7/DJ-1 is an oncogene that associated with tumorigenesis in many cancers. Recent studies have demonstrated the importance of DJ-1 origin and development uveal melanoma (UM). We present analysis role protein UM cells, especially its effect on proliferation migration. Methods: cells from a primary tumor, Mel 270, liver metastasis, OMM2.5, were transfected lentiviral-delivered shRNA against PARK7/DJ-1. Evaluation cell migration was performed using xCELLigence real-time...