A5059531830- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Endometriosis Research and Treatment
- Cancer Genomics and Diagnostics
- Reproductive Physiology in Livestock
- Tryptophan and brain disorders
- Immune Cell Function and Interaction
- Thyroid Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Diet and metabolism studies
- CAR-T cell therapy research
- Clinical Laboratory Practices and Quality Control
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Metabolism and Genetic Disorders
- Blood groups and transfusion
- Digestive system and related health
- Bipolar Disorder and Treatment
- Stress Responses and Cortisol
- Porphyrin Metabolism and Disorders
- COVID-19 Impact on Reproduction
- Immunotherapy and Immune Responses
- Adrenal and Paraganglionic Tumors
- Pregnancy and Medication Impact
- Lung Cancer Research Studies
Clinica Universidad de Navarra
2015-2025
Navarre Institute of Health Research
2016-2025
Universitat de València
2024
Universidad de Navarra
2004-2023
Institut d'Imagerie Biomédicale
2010
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2010
Molina Center for Energy and the Environment
2008
National Institutes of Health
1998
Walter Reed National Military Medical Center
1980
Naval Medical Research Command
1980
Malignant melanoma is an aggressive tumor that produces exosomes, which contain microRNAs (miRNAs) could be of utility in following tumoral cell dysregulation. MicroR-125b a miRNA whose down-regulation seems to implicated progression.To analyze miR-125b levels serum, and exosomes obtained from patients with advanced melanoma.Serum samples were 21 melanoma, 16 disease-free 19 healthy volunteers. Exosomes isolated serum by precipitation, miR-16 quantified real-time polymerase chain...
Abstract Background Exosomes are nanovesicles released by cells that can be detected in blood. contain several molecules, such as cytokines have potential utility disease biomarkers. The aim of the present work is to compare six different commercial kits suitable for clinical laboratory relation efficiency and purity exosome isolation, their effect subsequent analysis. Methods Serum exosomes were obtained from 10 volunteers using kits: exoEasy, ExoQuick, Exo-spin, ME kit, ExoQuick Plus...
The nonclassical human leukocyte antigen-G (HLA-G) is a tolerogenic molecule that can be released to the circulation by expressing cells. This form dimers but some other complexed HLA-G forms have been proposed present in vivo. Here, we further characterized these Ascitic and pleural exudates from patients were selected based on positivity for ELISA. Complexed was detected exosomes, which indicates an intracellular origin of forms. 2D-PAGE analysis isolated exosomes showed high molecular...
Mutation analysis of epidermal growth factor receptor (EGFR) gene is essential for treatment selection in non-small cell lung cancer (NSCLC). Analysis usually performed tumor samples. We evaluated the clinical utility EGFR plasma cell-free DNA (cfDNA) from patients under with inhibitors. selected 36 NSCLC and EGFR-activating mutations. Blood samples were collected at baseline during Wild-type EGFR, L858R, delE746-A750, T790M mutations quantified cfDNA by droplet digital PCR. Stage IV had...
In prolactinoma diagnosis, current guidelines recommend prolactin (PRL) assessment, considering values exceeding 200 ng/mL highly suggestive of prolactinoma. However, subtler hyperprolactinemia is more common, and to rule out potential prolactinomas, pituitary resonance magnetic imaging (MRI) studies are necessary. These present limitations in terms availability, costs, delays diagnosis. We aimed evaluate the screening utility metoclopramide (MCP) test identifying patients with moderate for...
The aim of this retrospective study was to analyse in advanced melanoma the potential tumor markers S-100B, inhibiting activity protein (MIA) and YKL-40 compared LDH. Serum levels MIA, LDH were measured 110 patients with (36 stage IIIB/C 74 IV), 66 disease-free 65 healthy controls. Results show that MIA significantly higher than or combination S-100B plus had best diagnostic sensitivity, addition did not further increase sensitivity. an independent prognostic factor overall survival....
Abstract Objectives Personalized reference intervals (prRI) have been proposed as a diagnostic tool for assessing measurands with high individuality. Here, we evaluate clinical performance of prRI using carcinoembryonic antigen (CEA) cancer detection and compare it that change values (RCV) other criteria recommended by guidelines (e.g. 25 % between consecutive CEA results (RV25) the cut-off point 5 μg/L (CP5)). Methods Clinical analytical data from 2,638 patients collected over 19 years were...
Human leucocyte antigen G (HLA-G) is a tolerogenic molecule that protects the fetus from maternal immune attack, may favour tumoral immunoescape and up-regulated in viral inflammatory diseases. The aim of this work was to discover if nitric oxide (NO) could affect HLA-G expression or function because NO an important modulator innate adaptive immunity. For purpose were analysed following treatment with donor peroxynitrite various cell lines expressing either spontaneously upon transfection....
Variegate porphyria (VP) results from haploinsufficiency of protoporphyrinogen oxidase (PPOX), the seventh enzyme in heme synthesis pathway. There is no VP model that recapitulates clinical manifestations acute attacks. Combined administrations 2-allyl-2-isopropylacetamide and rifampicin rabbits halved hepatic PPOX activity, resulting increased accumulation a potentially neurotoxic precursor, lipid peroxidation, inflammation, hepatocyte cytoplasmic stress. Rabbits also showed hypertension,...
Acute intermittent porphyria (AIP) is a rare metabolic disorder caused by haploinsufficiency of hepatic porphobilinogen deaminase (PBGD), the third enzyme heme biosynthesis. Individuals with AIP experience neurovisceral attacks closely associated overproduction potentially neurotoxic precursors.