A5036972191- Insect Resistance and Genetics
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Insect Utilization and Effects
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Nematode management and characterization studies
- Insect and Arachnid Ecology and Behavior
- Aquaculture disease management and microbiota
- Genetics, Aging, and Longevity in Model Organisms
- Bacteriophages and microbial interactions
- Animal and Plant Science Education
- Mass Spectrometry Techniques and Applications
- thermodynamics and calorimetric analyses
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Plasma Applications and Diagnostics
- Plant Disease Resistance and Genetics
- Marine Bivalve and Aquaculture Studies
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Cancer-related molecular mechanisms research
- Antimicrobial Peptides and Activities
- Myxozoan Parasites in Aquatic Species
Ankang City Central Hospital
2025
Hunan Normal University
2014-2024
Huazhong Agricultural University
2008-2021
Institute of Oceanology
2016-2019
Chinese Academy of Sciences
2008-2019
South China Sea Institute Of Oceanology
2016-2019
China University of Geosciences
2006
State Key Laboratory of Agricultural Microbiology
2005
Cell death plays an important role in host-pathogen interactions. Crystal proteins (toxins) are essential components of Bacillus thuringiensis (Bt) biological pesticides because their specific toxicity against insects and nematodes. However, the mode action by which crystal toxins to induce cell is not completely understood. Here we show that toxin triggers necrosis signaling pathway using Cry6Aa-Caenorhabditis elegans toxin-host interaction system, involves increase concentrations...
Plant-parasitic nematodes (PPNs) are piercing/sucking pests, which cause severe damage to crops worldwide, and difficult control. The cyst root-knot (RKN) sedentary endoparasites that develop specialized multinucleate feeding structures from the plant cells called syncytia or giant respectively. Within these produce tubes, act as molecular sieves with exclusion limits. For example, Heterodera schachtii is reportedly unable ingest proteins larger than 28 kDa. However, it unknown yet what...
Abstract Background Esophageal squamous cell carcinoma (ESCA) is a type of cancer that starts in the cells lining esophagus, tube connecting throat to stomach. It known for its aggressive nature and poor prognosis. Understanding key factors drive this crucial developing better diagnostic tools treatments. Methods Gene expression profiles ESCA were analyzed using Expression Omnibus (GEO) datasets (GSE23400, GSE29001, GSE92396, GSE1420) from GEO database. Differentially expressed genes (DEGs)...
Securing protein for the approximate 10 billion humans expected to inhabit our planet by 2050 is a major priority global community. Evidence has accrued over past 30 years that strongly supports and justifies sustainable use of insects as means produce products feed pets, livestock, poultry, aquacultured species. Researchers entrepreneurs affiliated with universities industries, respectively, from 18 nations distributed across North South America, Europe, Asia, Africa Australia contributed...
Saccharopolyspora spinosa is an important producer of antibiotic spinosad with clarified biosynthesis pathway but its complex regulation networks associated primary metabolism and secondary metabolites production almost have never been concerned or studied before. The proteomic analysis a novel CCTCC M206084 was performed aimed to provide global profile regulatory proteins. Two-dimensional-liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 1090, 1166, 701, 509 proteins from...
Eight new α-pyrones 1–8 and three known 9–11 were isolated from marine-derived Nocardiopsis strains SCSIO 10419, 04583, KS107. The structures of compounds elucidated by comprehensive spectral analyses. absolute configurations 4-deoxyphomapyrone C (1), 4-deoxy-11-hydroxyphomapyrone (3), 4-deoxy-7R-hydroxyphomapyrone (5), phomapyrone (11) determined TDDFT-ECD calculations for the solution conformers, which revealed that conformation side chain was decisive sign characteristic high-wavelength...
Aims: The objective of this work was to enhance the insecticidal activity or widen pesticidal spectrum a commercial Bacillus thuringiensis strain YBT1520. Methods and Results: A vegetative protein gene vip3Aa7, under control its native promoter cry3A promoter, subcloned into B. acrystalliferous BMB171 generate BMB8901 BMBvip respectively. It found that amount Vip3Aa7 produced by 3·2-fold more than BMB8901. Therefore, vip3Aa7 transformed toxicity resulting BMB218V against Spodoptera exigua...
This study was undertaken to determine the retention of Salmonella through Alphitobius diaperinus metamorphosis and its contribution, defecation, external contamination.Insects were exposed a tagged enterica evaluated for elimination. (i) Each day 3 weeks, filter collected frass from restrained insect analysis. (ii) Exposed larvae in closed container followed pupation, newly emerged adults examined their marker bacteria.Exposed produced Salmonella-positive an average 8 days, ranging 6 11...
Summary A set of expressed sequence tag‐simple repeat (EST‐SSR) markers the Pacific oyster, Crassostrea gigas , was developed through bioinformatic mining GenBank public database. As June 30, 2007, a total 5132 EST sequences from were downloaded and screened for di‐, tri‐ tetra‐nucleotide repeats, with criteria at minimum 5, 4 repeats three categories SSRs respectively. Seventeen polymorphic microsatellite characterized. Allele numbers ranged 3 to 10, observed expected heterozygosity values...
The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence P. TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity XaxAB binary toxin Xenorhabuds nematophila, which has both necrotic apoptotic activities in mammalian cells vitro. To evaluate biological activity Plu1961/Plu1962, their coding genes were cloned expressed Escherichia coli. Both Plu1961 Plu1962 as soluble protein...
The larvae of the black soldier fly (BSF), Hermetia illucens L., are commonly associated with decaying organic wastes. Over past 15 years, investigators in China have conducted extensive research exploring use BSF to recycle materials as a means protect environment, while producing products value, such protein and bioenergy. Initial efforts were based on strain from USA. However, since then, H. strains specimens collected Hubei Guangdong Provinces been established used models explore this...
Information regarding black soldier fly (Diptera: Stratiomyidae) adult biology is vital as this the life stage that produces eggs and thus drives population size. The goal of study was to determine key biological characteristics flies they age in relation to: (1) thermal preferences (T sel ) males females; (2) impact temperature on heat shock protein expression well (3) sperm count; (4) viability males. Aging significantly impacted male female preferences. Young (<24-h-old) preferred...
Butenyl-spinosyn, a secondary metabolite produced by Saccharopolyspora pogona, exhibits strong insecticidal activity than spinosyn. However, the low synthesis capacity and unknown metabolic characteristics of butenyl-spinosyn in wild-type S. pogona limit its broad application engineering. Here, we showed that exhibited increased glucose consumption ability growth rate compared with spinosa, but production was much lower To further elucidate mechanism these different phenotypes, performed...
Microbes, combined with insects, convert organic waste into products of value. Resulting insects can be harvested and used as a high-quality protein resource, while the residues fertiliser. Microbes play an important role in conversion process. This review’s aim was focused on how microbes promote such black soldier fly ( Hermetia illucens L.), house Musca domestica waxworm Plodia interpunctella ) yellow meal worm Tenebrio molitor to waste, also harmlessly reducing pollution. The novelty is...
The group of nematicidal crystal protein Cry6A shares very low identity and exhibits different structure with Cry5B, another well-studied produced by Bacillus thuringiensis. In this study, we assayed the susceptibility bre mutants (Caenorhabditis elegans resistance to Cry5B) Cry6Aa2 examined synergistic activity between Cry5Ba2. Our results show that all are susceptible on lethal activity, growth inhibition, fertility exhibit no cross-resistance Cry6Aa2. Moreover, combinations Cry5Ba2 serial...
Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide have been difficult because the relatively short half-life such substances. On other hand, can be synthetized at different times development, so samples stages to studied, further complicating analysis. A dual genomic proteomic approach would enhance our ability identify substances, particularily using mass...