A5100687487- Microbial Natural Products and Biosynthesis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Marine Sponges and Natural Products
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Magnetic Properties and Applications
- Synthetic Organic Chemistry Methods
- Magnetic properties of thin films
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Catalytic Processes in Materials Science
- RNA and protein synthesis mechanisms
- Fungal Biology and Applications
- Glycosylation and Glycoproteins Research
- Alkaloids: synthesis and pharmacology
- Magnetic Properties of Alloys
- Bioactive Compounds and Antitumor Agents
- Synthesis and Biological Activity
- Synthesis and Catalytic Reactions
- Shape Memory Alloy Transformations
- Zeolite Catalysis and Synthesis
- Mesoporous Materials and Catalysis
South China Sea Institute Of Oceanology
2016-2025
Institute of Oceanology
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2011-2025
University of Nottingham Ningbo China
2025
Beihang University
2021-2025
Peking University
2010-2025
University College London
2025
Kunming University of Science and Technology
2016-2024
British Columbia Children's Hospital
2024
The outbreak of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus continually lead to worldwide human infections and deaths. Currently, there is no specific viral protein-targeted therapeutics. Viral nucleocapsid protein a potential antiviral drug target, serving multiple critical functions during the life cycle. However, structural information remains unclear. Herein, we have determined 2.7 Å crystal structure N-terminal RNA binding domain protein. Although overall similar as other...
Glycosyltransferases (GTs), an essential class of ubiquitous enzymes, are generally perceived as unidirectional catalysts. In contrast, we report that four glycosyltransferases from two distinct natural product biosynthetic pathways-calicheamicin and vancomycin-readily catalyze reversible reactions, allowing sugars aglycons to be exchanged with ease. As proof the broader applicability these new more than 70 differentially glycosylated calicheamicin vancomycin variants reported. This study...
Abstract Four new indolo‐sesquiterpenes – dixiamycins A ( 1 ) and B 2 ), oxiamycin 3 chloroxiamycin 4 were isolated from a marine‐derived Actinomycete characterized, together with the known compound xiamycin 5 ). Dixiamycins are first examples of atropisomerism naturally occurring N‐N‐coupled atropo‐diastereomers, dimeric indolo‐sesquiterpene skeleton stereogenic N‐N axis between sp ‐hybridized nitrogen atoms. Solution TDDFT ECD calculations utilized to ascertain axial chirality , rotational...
Four new β-carboline alkaloids, designated marinacarbolines A–D (1–4), two indolactam 13-N-demethyl-methylpendolmycin (5) and methylpendolmycin-14-O-α-glucoside (6), the three known compounds 1-acetyl-β-carboline (7), methylpendolmycin (8), pendolmycin (9) were obtained from fermentation broth of Marinactinospora thermotolerans SCSIO 00652, a actinomycete belonging to family Nocardiopsaceae. Their structures elucidated by extensive MS 1D 2D NMR spectroscopic data analyses. The structure...
A PCR-based screening approach led to the identification of a deep-sea-derived Streptomyces sp. SCSIO 03032 capable producing new bisindole alkaloids spiroindimicins A–D (1–4). Structural elucidation these compounds revealed presence unusual [5,6] or [5,5] spiro-ring containing skeletons. Spiroindimicins B–D (2–4) with exhibited moderate cytotoxicities against several cancer cell lines.
Abstract Ikarugamycin is a member of the polycyclic tetramate macrolactams (PTMs) family natural products with diverse biological activities. The biochemical mechanisms for formation ring systems in PTMs remain elusive. enzymatic mechanism constructing an inner five‐membered ikarugamycin reported. A three‐gene‐cassette ikaABC from marine‐derived Streptomyces sp. ZJ306 sufficient conferring production heterologous host. IkaC catalyzes reductive cyclization reaction to form by Michael...
We report the activation of a PTM gene cluster in marine-derived <italic>Streptomyces pactum</italic>, leading to discovery six new PTMs, pactamides A-F.
Zoo animals, harboring zoonotic gastrointestinal protozoal diseases, pose potential hazards to the safety of visitors and animal keepers. This study involved collection examination 400 fresh fecal samples from 68 species, obtained five zoos. The aim this was determine occurrence, genetic characteristics, common protists. PCR or nested analysis conducted on these detect four specific parasites: Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, Blastocystis spp. overall...
It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.
Xiamycin A (XMA) and oxiamycin (OXM) are bacterial indolosesquiterpenes featuring rare pentacyclic ring systems isolated from a marine-derived Streptomyces sp. SCSIO 02999. The putative biosynthetic gene cluster for XMA/OXM was identified by partial genome sequencing approach. Eighteen genes were proposed to be involved in biosynthesis, including five terpene synthesis via non-mevalonate pathway, eight encoding oxidoreductases, regulation resistance. Targeted disruptions of 13 within the xia...
The RNA polymerase inhibitor tiacumicin B is currently undergoing phase III clinical trial for treatment of Clostridium difficile associated diarrhea with great promise. To understand the biosynthetic logic and to lay a foundation generating structural analogues via pathway engineering, gene cluster was identified characterized from producer Dactylosporangium aurantiacum subsp. hamdenensis NRRL 18085. Sequence analysis 110 633 bp DNA region revealed presence 50 open reading frames (orfs)....
The biosynthesis gene cluster (fls) for atypical angucycline fluostatins was identified from the marine derived Micromonospora rosaria SCSIO N160 and confirmed by knockouts biochemical characterization of a bifunctional oxygenase FlsO2. absolute configuration key biosynthetic intermediate prejadomycin determined first time Cu Kα X-ray analysis. Heterologous expression intact fls-gene in Streptomyces coelicolor YF11 presence 3% sea salts led to isolation two new compounds: fluostatin L (1)...
Over 200 genomes of streptomycete strains that were isolated from various environments are available the NCBI. However, little is known about characteristics linked to marine adaptation in marine-derived streptomycetes. The particularity and complexity environment suggest streptomycetes genetically diverse. Here, we sequenced nine Streptomyces genus different longitudes, latitudes, depths South China Sea. Then compared these 22 NCBI downloaded strains. Thirty-one clearly grouped into a...
Abstract (‒)-Morphine, which is selected as an essential medicine by World Health Organization, widely applied in the treatment of pain-related diseases. Due to its synthetically challenging molecular architecture and important clinical role, extensive synthetic studies morphine-type alkaloids have been conducted. However, catalytic asymmetric total synthesis (‒)-morphine remains a long-standing challenge. Here, we disclose efficient enantioselective longest linear sequence 16 steps. The key...
Currently, the synthesis of pure silica zeolites always requires presence organic structure-directing agents (OSDAs), which direct assembly pathway and ultimately fill pore space. A sustainable route is now reported for synthesizing in absence OSDAs from a combined strategy zeolite seeding alcohol filling, where seeds crystallization crystals amorphous silica, while served as filling zeolites. Very importantly, could be fully washed out pores by water at room temperature, completely avoids...
Abstract Lomaiviticin A and difluostatin are benzofluorene-containing aromatic polyketides in the atypical angucycline family. Although these dimeric compounds potent antitumor agents, how nature constructs their complex structures remains poorly understood. Herein, we report discovery of a number fluostatin type with varied C−C C−N coupling patterns. We also demonstrate that dimers not true secondary metabolites, but instead derived from non-enzymatic deacylation biosynthetic acyl...
Abstract Large and sensitive magnetostriction (large strain induced by small magnetic fields) is highly desired for applications of magnetostrictive materials. However, it difficult to simultaneously improve reduce the switching field because are both proportional magnetocrystalline anisotropy. To solve this fundamental challenge, we report that introducing tetragonal nanoprecipitates into a cubic matrix can facilitate large even in random polycrystals. As exhibited proof-of-principle...
Abstract The outbreak of coronavirus disease (COVID-19) in China caused by SARS-CoV-2 virus continually lead to worldwide human infections and deaths. It is currently no specific viral protein targeted therapeutics yet. Viral nucleocapsid a potential antiviral drug target, serving multiple critical functions during the life cycle. However, structural information yet be clear. Herein, we have determined 2.7 Å crystal structure N-terminal RNA binding domain protein. Although overall similar...