A5035853910- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Plant and Fungal Interactions Research
- Viral gastroenteritis research and epidemiology
- Complement system in diseases
- MicroRNA in disease regulation
- Plant Disease Management Techniques
- Phosphodiesterase function and regulation
- Plant Virus Research Studies
- SARS-CoV-2 detection and testing
- Epigenetics and DNA Methylation
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Plant Disease Resistance and Genetics
- Bone Tissue Engineering Materials
- S100 Proteins and Annexins
- Animal Virus Infections Studies
- Endodontics and Root Canal Treatments
- Oral microbiology and periodontitis research
- Forest Insect Ecology and Management
- Curcumin's Biomedical Applications
- Plant Pathogens and Fungal Diseases
- Cellular transport and secretion
- Mechanisms of cancer metastasis
Guangzhou Medical University
2021-2025
Stomatology Hospital
2021-2023
Sun Yat-sen University
2017-2022
Fifth Affiliated Hospital of Sun Yat-sen University
2020-2022
Imaging Center
2020
Huazhong Agricultural University
2016-2020
Shanghai Institutes for Biological Sciences
2014
Chinese Academy of Sciences
2014
Sichuan University
2009
The outbreak of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus continually lead to worldwide human infections and deaths. Currently, there is no specific viral protein-targeted therapeutics. Viral nucleocapsid protein a potential antiviral drug target, serving multiple critical functions during the life cycle. However, structural information remains unclear. Herein, we have determined 2.7 Å crystal structure N-terminal RNA binding domain protein. Although overall similar as other...
Dysregulated immune cell responses have been linked to the severity of coronavirus disease 2019 (COVID-19), but specific viral factors severe acute respiratory syndrome 2 (SARS-CoV-2) were currently unknown. Herein, we reveal that Immunoglobulin-like fold ectodomain protein SARS-CoV-2 ORF7a interacts with high efficiency CD14+ monocytes in human peripheral blood, compared pathogenic SARS-CoV ORF7a. The crystal structure at 2.2 Å resolution reveals three remarkable changes on amphipathic side...
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular in intact organisms are largely unknown due to low sensitivity and/or brightness current genetically encoded fluorescent indicators. Here, we report development new circularly permuted GFP (cpGFP)-based indicator G-Flamp1, which exhibits large fluorescence increase (a maximum ΔF/F
Abstract Although human antibodies elicited by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about function of N-reactive antibodies. Herein, we isolate and profile a panel 32 N protein-specific monoclonal (mAbs) from quick recovery disease-19 (COVID-19) convalescent patient who has dominant antibody responses to SARS-CoV-2 rather than spike (S) protein. The complex structure RNA binding domain...
Coronavirus disease 2019 (COVID-19) has caused massive disruptions to society and the economy, transcriptional regulatory mechanisms behind severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are poorly understood. Herein, we determined crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal domain (CTD) at a resolution 2.0 Å, demonstrated that CTD comparable distinct electrostatic potential surface equivalent domains other reported CoVs, suggesting novel roles in viral RNA...
Abstract The outbreak of coronavirus disease (COVID-19) in China caused by SARS-CoV-2 virus continually lead to worldwide human infections and deaths. It is currently no specific viral protein targeted therapeutics yet. Viral nucleocapsid a potential antiviral drug target, serving multiple critical functions during the life cycle. However, structural information yet be clear. Herein, we have determined 2.7 Å crystal structure N-terminal RNA binding domain protein. Although overall similar...
To study the effect of electrospinning nanofiber mats synthesized using hydroxypropyl chitosan in conjugation with poly (vinyl alcohol) on osteogenesis MC3T3 cell, we fabricated different ratios (HPCH) and to electrospin mats, mechanical physical properties were tested by SEM, FT-IR conventional methods. Then cell evaluated Alizarin Red stain, ALP stain expression relevant genes. We found that (50%) alcohol)(50%) can induce osteoblast differentiation apparently cell.
ORF8 is a viral immunoglobulin-like (Ig-like) domain protein encoded by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome. It tends to evolve rapidly and interfere with immune responses. However, structural characteristics of various proteins their subsequent effects on biological functions remain unclear. Herein, we determined crystal structures SARS-CoV-2 (S84) (one epidemic isoforms) bat RaTG13 variant at 1.62 Å 1.76 resolution, respectively. Comparison these...
Centromere protein W (CENP-W), identified as a centromeric component, plays an important role in the cell life cycle. However, how CENPW expression affects biological processes liver cancer cells remains unknown. In this article, we found that was overexpressed tissues. Low correlated with better prognosis hepatocellular carcinoma (HCC) patients, compared to high expression. The results of qRT-PCR and western blot assay showed effectively knocked down HCC using siRNA transfection. Cell...
Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to regulate cell biological behavior in some human malignancies. We hypothesized there was a negative regulation, which might induce apoptosis, between and PUMA adenoid cystic carcinoma (ACC). In this study, expression levels gene after transfection with antisense (As-miR-222) were evaluated by RT-PCR Western blot assays. Cell proliferation migratory abilities detected CCK-8 Transwell cycle...
The medical application of chitosan (Cs) has been for about half a century, but the molecular mechanism not elucidated yet. This study is to explore antibacterial nanoparticles (Csnp) in dentin at atomic resolution level. Extracted tooth specimen was prepared three groups: A. control group; B. Csnp treatment under ultrasonic agitation (UA); C. without UA. A scanning electron microscope (SEM) used observe distribution on surface. incubations Enterococcus faecalis (E. faecalis) were performed....
Dysregulated immune cell responses have been linked to the severity of Coronavirus Disease 2019 (COVID-19). However, specific viral factor severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contributing immune-dysregulation is currently unclear. Herein, we identified ectodomain Ig-like fold proteinSARS-CoV-2 Orf7a interacted with CD14+ monocytes at highest efficiency in human peripheral blood mononuclear cells, but not for relative highly pathogenic protein SARS-CoV Orf7a. The 2.2...
Abstract cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular in intact organisms are largely unknown due to low sensitivity and/or brightness current genetically encoded fluorescent indicators. Here, we report development new circularly permuted GFP (cpGFP)-based indicator G-Flamp1, which exhibits large fluorescence increase (a maximum ΔF/F 0 1100% HEK293T cells), relatively high...
A total of 707 isolates Botrytis were collected from plastic tunnel-grown strawberry and tomato in the Hubei province China. They identified based on specific molecular markers. Diversity B. cinerea (Bc) was evaluated by typing transposable elements (Boty, Flipper) mating types (MAT1-1, MAT1-2), as well determining virulence tobacco (Nicotiana benthamiana) fenhexamid sensitivity agar medium. The results showed that 706 (99.9%) Bc 1 isolate (0.1%) pseudocinerea. (n = 706) classified into four...
This study aims to explore the therapeutic targets of curcumin in periodontitis through network pharmacology and molecular docking technology.Targets were predicted by different databases, protein-protein interaction (PPI) constructed String revealed between periodontitis. The key target genes screened for Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses. Molecular was performed analyze binding potential periodontitis.A total 672 periodontitis-related disease 107...
Abstract Although human antibodies elicited by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about function of N-reactive antibodies. Herein, we isolated and profiled a panel 32 N protein-specific monoclonal (mAbs) from quick recovery disease-19 (COVID-19) convalescent patient who had dominant antibody responses to SARS-CoV-2 rather than spike (S) protein. The complex structure RNA binding...
Abstract Although human antibodies elicited by severe acute respiratory distress syndrome coronavirus-2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about the function of N-directed antibodies. Herein, we isolated and profiled a panel 32 N protein-specific monoclonal (mAb) from quick recovery coronavirus disease-19 (COVID-19) convalescent, who had dominant antibody responses to SARS-CoV-2 rather than Spike protein. The complex structure RNA...
The interaction of the solo H3K79 methyltransferase DOT1-like (DOT1L) and its regulatory factor ALL1-fused gene from chromosome 10 protein (AF10) is crucial for transcription developmental genes such as HOXA in acute leukemia. octapeptide motif leucine zipper region AF10 responsible binding DOT1L catalyzing monomethylation to demethylation. However, characteristics mechanism between are not clear. Here, we present crystal structures coiled-coil regions DOT1L-AF10 AF10-inhibitory peptide,...