A5102027329- Insect Resistance and Genetics
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Genetically Modified Organisms Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bacillus and Francisella bacterial research
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Mass Spectrometry Techniques and Applications
- Studies on Chitinases and Chitosanases
- Cancer Research and Treatments
- GABA and Rice Research
- Enzyme Structure and Function
- Plant Micronutrient Interactions and Effects
- Cassava research and cyanide
- Nanoplatforms for cancer theranostics
- Advanced Proteomics Techniques and Applications
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
Hunan Normal University
2013-2023
Chinese Academy of Sciences
2005-2008
Wuhan Institute of Virology
2005-2008
Traditional cancer therapies, such as surgery treatment, radiotherapy, and chemotherapy, often fail to completely eliminate tumor cells in an anaerobic microenvironment of regions. In contrast these traditional the use targeted delivery vectors deliver anticancer genes or antitumor drugs hypoxic areas tumors is most clinically promising treatment with rapid development recent years. this study, E.coli Nissle 1917 (EcN), intestinal probiotic, was utilized a transport vector p53 Tum-5 protein...
Tumor growth and metastasis depend on angiogenesis. Thus, inhibiting tumor angiogenesis has become promising cancer therapeutic strategy in recent years. Tumstatin is a more powerful inhibitor than endostatin. Anti-angiogenic active fragment encoding amino acids 45-132 (Tum-5) of tumstatin was subcloned into four different inducible expression vectors successfully solubly expressed Escherichia coli BL21 (DE3) this study. Subsequently, an anaerobic vector constructed under Vitreoscilla...
Saccharopolyspora spinosa is an important producer of antibiotic spinosad with clarified biosynthesis pathway but its complex regulation networks associated primary metabolism and secondary metabolites production almost have never been concerned or studied before. The proteomic analysis a novel CCTCC M206084 was performed aimed to provide global profile regulatory proteins. Two-dimensional-liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 1090, 1166, 701, 509 proteins from...
The total protoxin complement in the parasporal body of mosquitocidal strain, Bacillus thuringiensis subsp. jegathesan 367, was determined by use a polyacrylamide gel block coupled to mass spectrometry. A eight protoxins were identified from this including five reported (Cry11Ba, Cry19Aa, Cry24Aa, Cry25Aa, and Cyt2Bb), as well three previously undescribed (Cry30Ca, Cry60Aa, Cry60Ba) isolate. It interesting that encoding genes new existed cry30Ca-gap-orf2 cry60Ba-gap-cry60Aa. cry30Ca...
Abstract Background Butenyl-spinosyn, produced by Saccharopolyspora pogona , is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum. Bacterioferritin (Bfr, encoded bfr ) regulates the storage utilization of iron, which essential for growth metabolism microorganisms. However, effect Bfr on butenyl-spinosyn biosynthesis in S. has not been explored. Results Here, we found that intracellular iron influenced stress resistance was regulated Bfr. The...
The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence P. TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity XaxAB binary toxin Xenorhabuds nematophila, which has both necrotic apoptotic activities in mammalian cells vitro. To evaluate biological activity Plu1961/Plu1962, their coding genes were cloned expressed Escherichia coli. Both Plu1961 Plu1962 as soluble protein...
The proteome of a new Bacillus thuringiensis subsp. kurstaki strain, 4.0718, from the middle vegetative (T(1)), early sporulation (T(2)), and late (T(3)) phases was analyzed using an integrated liquid chromatography (LC)-based protein identification system. system comprised two-dimensional (2D) LC coupled with nanoscale electrospray ionization (ESI) tandem mass spectrometry (MS/MS) on high-resolution hybrid spectrometer automated data analysis After deletion redundant proteins different...
Abstract Background Saccharopolyspora spinosa produces the environment-friendly biopesticide spinosad, a mixture of two polyketide-derived macrolide active ingredients called spinosyns A and D. Therefore considerable interest is in improvement spinosad production because its low yield wild-type S. . Recently, spinosad-hyperproducing PR2 strain with stable heredity was obtained from protoplast regeneration SP06081 strain. comparative proteomic analysis performed on strains during first rapid...
Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide have been difficult because the relatively short half-life such substances. On other hand, can be synthetized at different times development, so samples stages to studied, further complicating analysis. A dual genomic proteomic approach would enhance our ability identify substances, particularily using mass...
Butenyl-spinosyn, a secondary metabolite produced by Saccharopolyspora pogona, exhibits strong insecticidal activity than spinosyn. However, the low synthesis capacity and unknown metabolic characteristics of butenyl-spinosyn in wild-type S. pogona limit its broad application engineering. Here, we showed that exhibited increased glucose consumption ability growth rate compared with spinosa, but production was much lower To further elucidate mechanism these different phenotypes, performed...
To improve ultraviolet (UV) resistance of Bacillus thuringiensis for increasing the duration Bt product applied in field, a genetically engineered strain TD841 that produced both melanin and Cry1A protein was constructed, its UV evaluated laboratory.Melanin quantitative analysis revealed recombinant could synthesize 0.15 mg ml(-1) sporulated culture. Atomic force microscopy confirmed production diamond crystal SDS-PAGE results showed expression 130 kDa protein. Bioassay demonstrated LC(50)...
Understanding the metabolism of Saccharopolyspora pogona on a global scale is essential for manipulating its metabolic capabilities to improve butenyl-spinosyn biosynthesis. Here, we combined multiomics analysis parse S. genomic information, construct network, and mine important functional genes that affect This research not only elucidated relationship between biosynthesis primary pathway but also showed low expression level continuous downregulation bus cluster competitive utilization...
By a combination of PCR and mass spectrometry, total five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, three protoxins (Cry1Aa, Cry1Ac, Cry2Aa) identified strain's parasporal crystals. These results indicated that this complementary method may be useful evaluating B. strains at both gene protein levels.
The growth process of Bacillus thuringiensis Bt4.0718 strain was studied using proteomic technologies. proteins Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed separation by 2-DE identified MALDI-TOF/TOF MS. Bioactive factors such as insecticidal crystal (ICPs) including Cry1Ac(3), Cry2Aa, BTRX28, immune inhibitor (InhA), InhA precursor were identified. started to express the middle vegetative phase,...
Summary Butenyl‐spinosyn, a promising biopesticide produced by Saccharopolyspora pogona , exhibits stronger insecticidal activity and broader pesticidal spectrum. However, its titre in the wild‐type S. strain is too low to meet industrial production requirements. Deletion of non‐target natural product biosynthetic gene clusters resident genome could reduce consumption synthetic precursors, thereby promoting biosynthesis butenyl‐spinosyn. it has always been challenge for scientists...
Syringolin, synthesized by a mixed non-ribosomal peptide synthetase/polyketide synthetase in Pseudomonas syringae pv. (Pss) B728a, is novel eukaryotic proteasome inhibitor. Meanwhile, directly modifying large fragments the PKS/NRPS gene cluster through traditional DNA engineering techniques very difficult. In this study, we cloned syl from Pss B301D-R via Red/ET recombineering to effectively express syringolin heterologous hosts. A 22 kb genomic fragment containing sylA–sylE was into pASK...
Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression the effects of these elements vary significantly. However, molecular mechanisms caused by have yet to be reported. The ICP are mainly composed Cry1Ca, Cry1Ac, Cry1Da, which weights about 130 kDa. production was most efficient when Cu2+ added at concentrations ranging...