A5071919859- Antibiotic Use and Resistance
- Probiotics and Fermented Foods
- Gut microbiota and health
- Tuberculosis Research and Epidemiology
- Pneumonia and Respiratory Infections
- Healthcare Systems and Reforms
- Patient Satisfaction in Healthcare
- Bacterial Genetics and Biotechnology
- Global Maternal and Child Health
- Drug Transport and Resistance Mechanisms
- Insect Resistance and Genetics
- Chronic Disease Management Strategies
- Antibiotic Resistance in Bacteria
- Pharmacological Effects and Toxicity Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumocystis jirovecii pneumonia detection and treatment
- Allergic Rhinitis and Sensitization
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Pharmaceutical Practices and Patient Outcomes
- Legume Nitrogen Fixing Symbiosis
Yangzhou University
2025
Hunan Normal University
2011-2024
Shandong University
2015-2024
Central South University
2024
Nankai University
2021-2024
Shandong First Medical University
2021-2023
Shandong Provincial QianFoShan Hospital
2023
Peking Union Medical College Hospital
2012-2022
National Clinical Research Center for Digestive Diseases
2021-2022
Center for Disease Control
2022
The aim of this study was to assess the reliability and validity 6-min walk test (6MWT) in healthy children. Chinese secondary school students were randomly recruited. They attended current authors' unit on two occasions, separated by 2 weeks. Physical examination standardised maximum incremental exercise testing a treadmill performed first visit. Spirometry 6MWT carried out second A selected subgroup invited return for repeat at an interval 2–4 Seventy-eight subjects recruited; however,...
Gut dysbiosis and bile acid (BA) metabolism disturbance are involved in the pathogenesis of ulcerative colitis. This study aimed to investigate effect fucoidan on BA gut microbiota dextran sulfate sodium-induced colitis mice. Our results showed that effectively suppressed colonic inflammation repaired barrier. In addition, increased relative abundance Lachnospiraceae family, such as Turicibacter, Muribaculum, Parasutterella, Colidextribacter, followed by an increase short-chain fatty acids,...
The exponentially increasing volumes of DNA sequence data highlight the need for new cloning methods to explore information. Here, we describe 'ExoCET' (Exonuclease Combined with RecET recombination) directly clone any chosen region from bacterial and mammalian genomes nucleotide precision into operational plasmids. ExoCET combines in vitro exonuclease annealing remarkable capacity full length homologous recombination (HR) retrieve specified regions genomic preparations. Using T4 polymerase...
BackgroundInappropriate antibiotic prescribing contributes to the generation of drug resistance worldwide, and is particularly common in China. We assessed effectiveness an antimicrobial stewardship programme aiming reduce inappropriate paediatric outpatients by targeting providers caregivers primary care hospitals rural China.MethodsWe did a pragmatic, cluster-randomised controlled trial with 6-month intervention period. Clusters were township two counties Guangxi province China, which...
Background: The rapidly increasing dissemination of carbapenem-resistant Enterobacteriaceae (CRE) in both humans and animals poses a global threat to public health. However, the transmission CRE between has not yet been well studied. Objectives: We investigated prevalence, risk factors, drivers their backyard rural China. Methods: conducted comprehensive sampling strategy 12 villages Shandong, Using household [residents (farm companion animals)] as single surveillance unit, we assessed...
Abstract Background Probiotics are being considered as valuable microorganisms related to human health. Hu sheep is referred one of the important breeds in China. Goat milk produced by characterized with high nutritional value and hypoallergenic nature. Particularly, this contains plenty prebiotic probiotic bacteria. This study was aimed scrutinize more bacterial strains from potential activity. Results Based on 16S rRNA sequence analysis, pool forty were identified evaluated their...
Inappropriate antibiotic prescribing causes widespread serious health problems. To reduce of antibiotics in Chinese primary care to children with upper respiratory tract infections (URTIs), we developed an intervention comprising clinical guidelines, monthly review meetings, doctor-patient communication skills training, and education materials for caregivers. We previously evaluated our using unblinded cluster-randomised controlled trial (cRCT) 25 facilities across two rural counties. When...
Scope Tryptophan (Trp) metabolites are closely related to neurological diseases, whereas, the underlying mechanism alleviative effects of Trp on neurodegeneration in aging remains unclear. This study aims evaluate protective and mechanisms process. Methods results The neuroprotective properties evaluated vitro vivo experimental model. such as indole, indole‐3‐acetic acid (IAA), indole‐3‐propionic (IPA), indole‐3‐lactic (ILA), indole‐3‐carboxyaldehyde (Icld) could significantly reduce...
Chronic obstructive pulmonary disease (COPD), a condition primarily linked to oxidative stress, poses significant health burdens worldwide. Recent evidence has shed light on the association between dysbiosis of gut microbiota and COPD, their metabolites have emerged as potential modulators progression through intricate gut-lung axis. Here, we demonstrate efficacy oral administration probiotic Pediococcus pentosaceus SMM914 (SMM914) in delaying COPD by attenuating stress. Specially, induces...
Abstract Bacterial competent cells are essential for cloning, construction of DNA libraries, and mutagenesis in every molecular biology laboratory. Among various transformation methods, electroporation is found to own the best efficiency. Previous methods based on washing electroporating bacterial ice-cold condition that make them fragile prone death. Here we present simple temperature shift improve recombineering efficiency E. coli several other gram-negative bacteria thereby economizing...
Self-management is the cornerstone of diabetes care, however, despite numerous recommendations available for self-management, type-2 mellitus (T2DM) patients' performance suboptimal in China. This study aimed to explore association between self-efficacy and self-management behaviors among Chinese T2DM patients, which might provide evidence inform effective interventions these patients.A cross-sectional survey was conducted using a multi-stage stratified randomized sampling Shandong Province,...
Dietary tryptophan affects intestinal homeostasis and neurogenesis, whereas the underlying mechanism reciprocal interaction between gut microbiota in aging are unclear. This investigation was performed to determine effect of on intestinal- neuro- health aging. In present study, 0.4% diet significantly ameliorated oxidative stress inflammation mice, potentially through regulation 5'-adenosine monophosphate (AMP)-activated protein kinase (AMPK) nuclear factor κB (NF-κB) pathways. The increased...
The imbalance of reactive oxygen species is the main cause in aging, accompanied by oxidative stress. As most abundant human milk oligosaccharides (HMOs), 2'-Fucosyllactose (2'-FL) has been confirmed to have great properties immunity regulation and anti-inflammatory. research on 2'-FL focused infants currently, while there no related report for elderly. A d-galactose-induced accelerated aging model was established explore protective effect intestines brain mice. In this study, significantly...
Abstract Background In modern animal husbandry, breeders pay increasing attention to improving sow nutrition during pregnancy and lactation favor the health of neonates. Sow milk is a main food source for piglets their first three weeks life, which not only rich repository essential nutrients broad range bioactive compounds, but also an indispensable commensal bacteria. Maternal microorganisms are important sources bacteria neonatal gut. Bacteria from maternal may confer benefit on host....
Akkermansia muciniphila is a potential candidate for ulcerative colitis prevention. Considering that it utilizes 2′-fucosyllactose (2′FL) growth, 2′FL can be used to enrich the abundance of A. in feces. However, whether crosswalk between and promote intestinal colonization remains unclear. In this study, we explored effect underlying mechanism on vitro vivo as well its alleviating colitis. Our results revealed serve carbon source support growth increase cell-surface hydrophobicity expression...
<b>Objective:</b> To establish the association between airway inflammation and severity of obstructive sleep apnoea (OSA) in children. <b>Methods:</b> Consecutive children presenting with symptoms suggestive OSA were recruited. They completed a symptom questionnaire, underwent physical examination, spirometry, sputum induction an overnight polysomnography. Adequate contained <50% squamous epithelial cells, was diagnosed if index >1. <b>Results:</b> 73 median (interquartile range (IQR))...
Precise and fluent genetic manipulation is still limited to only a few prokaryotes. Ideally the highly advanced technologies available in Escherichia coli could be broadly applied. Our efforts apply lambda Red technology, widely termed 'recombineering', Photorhabdus Xenorhabdus yielded success. Consequently we explored properties of an endogenous luminescens Red-like operon, Plu2934/Plu2935/Plu2936. Bioinformatic functional tests indicate that Plu2936 5'-3' exonuclease equivalent Redα...
Abstract Linear plus linear homologous recombination-mediated recombineering (LLHR) is ideal for obtaining natural product biosynthetic gene clusters from pre-digested bacterial genomic DNA in one or two steps of recombineering. The salinomycin has a potent and selective activity against cancer stem cells therefore potential anti-cancer drug. Herein, we separately isolated three fragments the cluster ( sal O -orf 18) Streptomyces albus S. ) DSM41398 using LLHR assembled them into intact (106...