A5101778368- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Insect Resistance and Genetics
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Phytochemical compounds biological activities
- Innovative Energy Harvesting Technologies
- Fungal and yeast genetics research
- Dam Engineering and Safety
- Cancer Treatment and Pharmacology
- Soil and Unsaturated Flow
- Studies on Chitinases and Chitosanases
- Toxin Mechanisms and Immunotoxins
- Peptidase Inhibition and Analysis
- Sesquiterpenes and Asteraceae Studies
- Advanced Glycation End Products research
- Microbial Metabolism and Applications
- Biochemical Acid Research Studies
- Bacillus and Francisella bacterial research
- Wireless Power Transfer Systems
- Allelopathy and phytotoxic interactions
- Heat shock proteins research
- Polyamine Metabolism and Applications
- Characterization and Applications of Magnetic Nanoparticles
Guangxi University
2021-2022
Harvard University Press
2014
Brookhaven National Laboratory
2014
Hunan Normal University
2008-2009
Rice University
1999-2003
Aptose Biosciences (Canada)
2003
Texas A&M University
1998-2002
McGill University
2000
Jewish General Hospital
2000
U.S. Salinity Laboratory
1996
The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps most notorious member of nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential biosynthesis. Comparison calicheamicin with encoding chromoprotein C-1027 reveals PKS highly conserved among these distinct families. Contrary previous hypotheses, this suggests are generated by similar...
Abstract Efficiently capturing energy from environmental vibrations remains a significant challenge in sustainable systems. This paper presents novel integrated vibrational harvesting-storage-injection system (VEHSIS) to improve capture efficiency. The VEHSIS comprises four main components: an Energy Harvesting Module, Storage Injection and Microcontroller Control Module. employs four-magnet piezoelectric bistable structure, which decreases the height of potential barriers thus enables...
Aspergillus terreus dihydrogeodin oxidase (DHGO) is an enzyme catalyzing the stereospecific phenol oxidative coupling reaction converting to (+)- geodin. We previously reported purification of DHGO from A. and raised polyclonal antibody against DHGO. From first cDNA library constructed in lambda gt11 using mRNA 3-day-old mycelium terreus, four clones were identified anti-DHGO antibody, but all contained partial inserts around 280 base pairs. This fragment was used as a probe clone genomic...
A search for genes encoding enzymes involved in cobalamin (vitamin B12) production the commercially important organism Propionibacterium freudenreichii (P. shermanii) has resulted isolation of an additional 14 responsible 17 steps anaerobic B12 pathway this organism. All believed to be necessary biosynthesis adenosylcobinamide from uroporphyrinogen III have now been isolated except two (cbiA and as yet unidentified gene cobalt reductase). Most are contained divergent operons, one which,...
Inhibitor 1 (I-1) is a protein inhibitor of phosphatase (PP1), major eukaryotic Ser/Thr phosphatase. Nonphosphorylated I-1 inactive, whereas phosphorylated potent PP1 inhibitor. in vivo on Thr 35 and Ser 67 . by cAMP-dependent kinase (A kinase), -phosphorylated inhibits PP1. Until now the that phosphorylates had not been identified physiological role phosphorylation was unknown. In this study we detected high level activity brain extract when glutathione S -transferase (GST) fusion mutant...
ABSTRACT A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme overexpressed Escherichia coli and purified. Methylglyoxal has native molecular mass of 60 kDa an optimum pH 7.5. K m V max values for the substrate dihydroxyacetone phosphate were 0.53 mM 1.56 mmol min −1 μg , respectively. When E. glycerol dehydrogenase coexpressed with BL21(DE3), 3.9 1,2-propanediol produced.
A relatively simple method of characteristics is developed to simulate one‐dimensional variably saturated water flow. The uses the Eulerian‐Lagrangian approach separate governing flow equation into “convection” and “diffusion” parts, which are solved with conventional finite element method, respectively. combines a single‐step reverse particle tracking technique correction strategy ensure accurate mass balances. process implemented by weighing calculated convective contribution pressure head...
Analytic expressions for the potentials and fields of flat cylindrical plates, including fringe fields, are given. The present analysis extends simplifies current expression plates develops in terms polar coordinates. development a fortran program to output field strength at given location within Proton Electric Dipole Moment (Proton EDM) ring is then described. Fourth-order Runge-Kutta integration used investigate effect on particle spin dynamics with precision tracking proposed EDM experiment.
To enhance the harvesting performance of a bi-stable piezoelectric energy harvester (BEH), this work proposes four-magnet BEH (FBEH).FBEH consists cantilever beam with tip magnet, fixed magnet and two movable magnets.The magnets relative to can move in both horizontal vertical directions.A nonlinear distributed parameter model FBEH is derived through Hamilton principle.The effects excitation frequency amplitude as well gap on are mainly investigated by using bifurcation diagram, phase...
Spinosad (spinosyn A and spinosyn D), the secondary metabolite produced by Saccharopolyspora spinosa, is a potent insecticide with low effects on environment mammals. Strategies such as metabolic engineering, mutagenesis fermentation process optimization have been employed for its production enhancement. Quantitative real-time polymerase chain reaction(qRT-PCR) one of preferred methods evaluation gene transcript levels, whose accuracy sensitivity depend normalization using optimal reference...
Saccharopolyspora spinosa is a well-known actinomycete for producing the secondary metabolites, spinosad, which potent insecticides possessing both efficiency and safety. In previous researches, great efforts, including physical mutagenesis, fermentation optimization, genetic manipulation other methods, have been employed to increase yield of spinosad hundreds folds from low-yield strain. However, metabolic network in S. still remained un-revealed. this study, two strains with different...