A5010126262- Porphyrin Metabolism and Disorders
- Folate and B Vitamins Research
- Porphyrin and Phthalocyanine Chemistry
- Photosynthetic Processes and Mechanisms
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Neonatal Health and Biochemistry
- Chemical Synthesis and Analysis
- Heme Oxygenase-1 and Carbon Monoxide
- Molecular spectroscopy and chirality
- Plant biochemistry and biosynthesis
- Enzyme Structure and Function
- Cancer Treatment and Pharmacology
- Analytical Chemistry and Chromatography
- Synthetic Organic Chemistry Methods
- Fluorine in Organic Chemistry
- Carbohydrate Chemistry and Synthesis
- Chemical synthesis and alkaloids
- DNA and Nucleic Acid Chemistry
- Metabolism and Genetic Disorders
- Protein Structure and Dynamics
- Alkaloids: synthesis and pharmacology
- Synthesis of β-Lactam Compounds
- Plant tissue culture and regeneration
- Oxidative Organic Chemistry Reactions
Agri Food and Biosciences Institute
2023-2024
University of Ulster
2023-2024
Texas A&M University
1999-2008
Texas A&M University – Texarkana
2003
Mitchell Institute
1989-2000
Agricultural Research Service
1997-1998
Universidad de la República
1997
United States Department of Agriculture
1997
Bryn Mawr College
1994
American Chemical Society
1993
A. I. Scott, Q. Rev. Chem. Soc., 1965, 19, 1 DOI: 10.1039/QR9651900001
To support profitable agricultural production, nutrients, including phosphorus (P) are applied to soils. However, avoid over-application and mobilisation of excess P, in-soil concentrations must be maintained at the agronomic optimum (crop requirement) through soil test P (STP) data. Areas above STP (e.g., Olsen P) status have been linked elevated instream soluble reactive (SRP) concentrations. For example, when this is combined with hydrologically sensitive areas (HSAs), can mobilised...
The Escherichia coli cysG gene was successfully subcloned and over-expressed to produce a 52 kDa protein that purified homogeneity. This shown catalyse the S-adenosylmethionine-dependent methylation of uroporphyrinogen III give product identified as sirohydrochlorin on basis its absorption spectra, incorporation 14C label from S-adenosyl[Me-14C]methionine mass 1H-n.m.r. spectra octamethyl ester. Further confirmation structure obtained 14C-n.m.r. spectrum methyl ester produced by incubation...
In Bacillus megaterium, the hemAXBCDL genes were isolated and found to be highly similar from subtilis that are required for conversion of glutamyl-tRNA into uroporphyrinogen III. Overproduction purification HemC (porphobilinogen deaminase) -D (uroporphyrinogen III synthase) allowed these enzymes used in vitro synthesis porphobilinogen. A second smaller cluster three (termed sirABC) was also encode catalyse transformation sirohaem on basis their ability complement a defined Escherichia coli...
The C-terminus of the Escherichia coli CysG protein, consisting amino acids 202-457, was expressed as a recombinant protein using gene dissection methodology. Analysis activity this truncated termed CysGA, revealed that it able to methylate uroporphyrinogen III in same S-adenosyl-L-methionine (SAM)-dependent manner complete protein. However, not complement E. cysG cells, thereby suggesting first 201 had an enzymic associated with conversion dihydrosirohydrochlorin into sirohaem. N-terminus...
Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III produce precorrin‐2, hence it was given trivial name methylase. We now report that in addition methylase activity, CysG protein catalyses both NAD + dependent oxidation of precorrin‐2 sirohydrochlorin, but also insertion iron into this oxidized intermediate, thereby producing siroheme. Thus is a multifunctional solely responsible for siroheme synthesis from , and accordingly renamed synthase.
Strictosidine synthetase, which catalyzes the condensation of tryptamine with secologanin to form strictosidine (isovincoside), was purified 740-fold homogeneity from cultured cells Catharanthus roseus in 10% yield. The specific activity is 5.85 nkat/mg. molecular weight as estimated by gel filtration 38,000. isoelectric point 4.6. Apparent Km values for and are 0.83 0.46 mM, respectively. enzyme shows a broad pH optimum between 5.0 7.5. product enzymic reaction exclusively strictosidine,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation and Structure of HetacillinG. A. Hardcastle Jr., D. Johnson, C. Panetta, I. Scott, S. SutherlandCite this: J. Org. Chem. 1966, 31, 3, 897–899Publication Date (Print):March 1, 1966Publication History Published online1 May 2002Published inissue 1 March 1966https://pubs.acs.org/doi/10.1021/jo01341a060https://doi.org/10.1021/jo01341a060research-articleACS PublicationsRequest reuse permissionsArticle Views202Altmetric-Citations54LEARN...
In the vitamin B12 biosynthetic pathway enzymes responsible for conversion of precorrin-3 to precorrin-4 have been identified as gene products cobG and cobJ from Pseudomonas denitrificans. CobG catalyzes oxidation precorrin-3x (a hydroxy lactone) whereas CobJ is a SAM-dependent C-17 methyl transferase necessary ring contraction. A mechanism contraction proposed.
The mevalonate-dependent pathway is used by many organisms to synthesize isopentenyl pyrophosphate, the building block for biosynthesis of biologically important compounds, including farnesyl dolichol, and sterols. Mevalonate kinase (MVK) catalyzes a critical phosphoryl transfer step, producing mevalonate 5'-phosphate. crystal structure thermostable MVK from Methanococcus jannaschii has been determined at 2.4 A, revealing an overall fold similar homoserine M. jannaschii. In addition, enzyme...
Abstract This review contains an account of recent experimental results in the continuing saga vitamin B 12 biosynthesis (largely from author's laboratory) as well a personal view future directions research natural product biosynthesis. The emphasis is on powerful combination molecular biology search for, and expression of, genes encoding biosynthetic enzymes state‐of‐the‐art spectroscopic techniques, order to “view” biochemical events they take place NMR tube. As logical development these...
Uroporphyrinogen III methylase was purified from a recombinant hem B − strain of E. coli harbouring plasmid containing the cys G gene. N‐terminal analysis this protein gave an amino acid sequence corresponding to that predicted genetic code. From u.v./visible spectrum reaction catalysed by SAM dependent it possible observe sequential appearance chromophores dipyrrocorphin and subsequently pyrrocorphin. Confirmation transformation obtained 13 C‐NMR studies when demonstrated, for first time...
Evidence is presented from 13C n.m.r. spectroscopic studies which indicates that the enzymic transformation of porphobilinogen into uroporphyrinogens I and III occurs through a transient free intermediate, pre-uroporphyrinogen, produced by deaminase (uroporphyrinogen synthetase).
Pre-uroporphyrinogen, produced from porphobilinogen by the action of deaminase has been shown to act as a substrate for enzyme uroporphyrinogen III cosynthetase, being converted in high yield into III; absence pre-uroporphyrinogen rearranges chemically afford exclusively I, and evidence is also presented which demonstrates that cosynthetase function independently sequentially overall conversion III.
D. H. R. Barton and A. I. Scott, J. Chem. Soc., 1958, 1767 DOI: 10.1039/JR9580001767
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Sesquiterpene α-Methylene Lactones from the Egyptian Plant Jasonia candicansAhmed A. Ahmed, Ahmed Mahmoud, Howard J. Williams, Ian Scott, Joseph H. Reibenspies, and Tom MabryCite this: Nat. Prod. 1993, 56, 8, 1276–1280Publication Date (Print):August 1, 1993Publication History Published online1 July 2004Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/np50098a011https://doi.org/10.1021/np50098a011research-articleACS...