A5050729207- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Plant-based Medicinal Research
- Virus-based gene therapy research
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- Electrospun Nanofibers in Biomedical Applications
- Nicotinic Acetylcholine Receptors Study
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Forensic Toxicology and Drug Analysis
- Biopolymer Synthesis and Applications
- Ion channel regulation and function
- Marine Toxins and Detection Methods
- Insect and Pesticide Research
- Advanced Synthetic Organic Chemistry
- Synthetic Organic Chemistry Methods
- Alkaloids: synthesis and pharmacology
- Amino Acid Enzymes and Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Chemical synthesis and alkaloids
- Neurobiology and Insect Physiology Research
- Silk-based biomaterials and applications
- Graphene and Nanomaterials Applications
University of Bath
2014-2024
University of Glasgow
2023
University of Nottingham
1982-2019
Institute of Nanotechnology
2019
National Nanotechnology Research Center
2019
Bilkent University
2019
University College London
2019
Biopharma Technology (United Kingdom)
2009
University of Sunderland
2005
University of London
2005
The olfactory region of the nasal cavity directly links brain to external environment, presenting a potential direct route central nervous system (CNS). However, targeting drugs is challenging and relies on combination drug formulation, delivery device, administration technique navigate human anatomy. In addition, in vitro vivo models utilized evaluate performance formulations do not accurately reflect deposition uptake cavity. current study describes development respirable...
Abstract [ 3 H]‐Methyllycaconitine ([ H]‐MLA) is a new radioligand with selectivity for α7‐type neuronal nicotinic acetylcholine receptors (nAChRs). In our previous study [Davies, A.R.L., Hardick, D.J., Blagbrough, I.S., Potter, B.V.L., Wolstenholme, A.J. & Wonnacott, S. (1999) Neuropharmacology, 38, 679–690], this labelled single class of site in rat brain membranes; its pharmacology and distribution crudely dissected regions closely paralleled that the well‐established α7‐ligand 125...
To investigate the destruction of clinically-relevant bacteria within biofilms via sustained release antibiotic tetracycline from zein-based electrospun polymeric fibrous matrices and to demonstrate compatibility such wound dressing with human skin cells. Zein/PCL triple layered dressings entrapped were electrospun. The successful entrapment in these was validated. bioactive tetracycline, preformed biofilms, viability fibroblast (FEK4) cells investigated. led efficient Staphylococcus aureus...
Polyamines are believed to be potent vectors for the selective delivery of chemotherapeutic agents into cancer cells. In this paper, we report effect spermine conjugation on cytotoxic and transport properties acridine. Six derivatives, composed a chain attached at its N1 position an acridine via aliphatic chain, were synthesized. The linker, comprised 3−5 methylene units, was connected position-9 heterocycle through either amide (amidoacridines 8−10) or amine (aminoacridines 11−13) linkage....
Methyllycaconitine (MLA, 1) is a novel, potent probe for mammalian and insect nicotinic acetylcholine receptors (nAChR) displays remarkable selectivity toward neuronal [125I]-α-bungarotoxin (αBgTX) binding sites that correspond to α7-type nAChR in brain. We have shown that, among number of selected norditerpenoid alkaloids, elatine (2) nudicauline (3) are equipotent with, or better than, MLA (1) brain [125I]-αBgTX sites, with IC50 values 6.1, 1.7, 7.6 nM, respectively. The...
Synthetic cannabinoid receptor agonists (SCRAs) are one of the fastest growing classes recreational drugs. Despite their growth in use, vast chemical diversity and rapidly changing landscape structures make understanding effects challenging. In particular, side for SCRA use extremely diverse, but notably include severe outcomes such as cardiac arrest. These appear at odds with main putative mode action, full receptors. We have hypothesized that SCRAs may act MAO inhibitors, owing to...
Novel polyamine carbamates have been designed and prepared from cholesterol. Our synthesis uses an orthogonal protection strategy based upon trifluoroacetyl Boc-protecting groups. These unsymmetrical symmetrical (e.g., spermine thermine) polyamines. Detailed interpretations of (1)H (13)C NMR spectroscopic data led to the unambiguous assignment these carbamates. target conjugates contain a variety positive charges distributed along methylene chains. Their pK(a)s determined potentiometrically...
New therapeutic options are urgently required for the treatment of Staphylococcus aureus infections. Accordingly, we sought to exploit vulnerability S. naturally occurring polyamines. We have developed and tested anti-staphylococcal activity three novel linear polyamines based on spermine norspermine. Using a panel genetically distinct clinically relevant multidrug resistant isolates, including polyamine USA300 strain LAC, compound AHA-1394 showed greater than 128-fold increase in inhibition...
A general procedure to prepare gold nanourchins (GNUs) via a seed-mediated method was followed using dopamine hydrochloride as reducing agent and silver nitrate salt (AgNO3) shape-directing agent. The novelty of this study comes from the successful incorporation prepared urchins an aqueous suspension in nasal pressurized metered dose inhaler (pMDI) formulation investigation their potential for olfactory targeting direct nose-to-brain drug delivery (NTBDD). developed pMDI composed 0.025% w/w...
We have quantified the effects of regiochemical distribution positive charges along polyamine moiety in lipopolyamines for DNA molecular recognition. High affinity binding leads to charge neutralisation, condensation and ultimately lipofection. Binding affinities calf thymus were determined using an ethidium bromide displacement assay was detected by changes turbidity light scattering. The vitro transfection competence cholesterol carbamates measured CHO cells. In design condensing...