A5039992599- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Phytochemical compounds biological activities
- Pharmacological Effects of Natural Compounds
- Fungal Biology and Applications
- Phytochemicals and Antioxidant Activities
- Natural product bioactivities and synthesis
- Plant chemical constituents analysis
- Phytochemistry and Biological Activities
- Plant biochemistry and biosynthesis
- Bioactive Compounds and Antitumor Agents
- Heme Oxygenase-1 and Carbon Monoxide
- Traditional Chinese Medicine Analysis
- Neuroscience of respiration and sleep
- Synthesis of Organic Compounds
- Ginseng Biological Effects and Applications
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Berberine and alkaloids research
- Plant Virus Research Studies
- Saffron Plant Research Studies
- Plant-based Medicinal Research
- Dermatology and Skin Diseases
- Restraint-Related Deaths
- Alkaloids: synthesis and pharmacology
Makino Botanical Garden
2015-2023
Nagoya City University
2009-2022
Tokai University
2013-2022
Kanazawa Medical University
2015-2021
Tokyo Medical Examiner's Office
2010-2019
Tokai University Hachioji Hospital
2015-2016
Pharmac
2004-2015
Tokyo Metropolitan Government
2010-2015
Tokyo Medical University
2004-2014
Aichi Gakuin University
2010
Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes other lytic organelles. Motivated such aim, we have investigated capability multiwalled carbon nanotubes (MWCNTs) penetrate cell membrane plant protoplasts (plant cells made devoid their walls via enzymatic treatment) studied internalization mechanism confocal imaging TEM techniques. Our results indentified an...
Catharanthus roseus cell suspension cultures converted exogenously supplied curcumin to a series of glucosides, none which has been found in nature so far. The efficiency glucosylation was dependent on culture stage the cells and medium sucrose concentration. Methyl jasmonate salicylic acid enhanced glucoside formation only when they were added prior addition curcumin. yield 2.5 μmol/g fresh weight at an optimal condition. water solubility curcumin‐4′,4″‐ O ‐β‐ D ‐digentiobioside 0.65...
Quercetin, a flavonol contained in various vegetables and herbal medicines, has biological activities including anti-cancer, anti-allergic anti-oxidative activities. However, low oral bioavailability of quercetin due to insolubility water limited its use as food additive or dietary supplement. Since the solubility is enhanced by glycosyl conjugation, present study, we evaluated several glycosides with different sugar moieties rats. quercetin-3-O-rutinoside (rutin), quercetin-3-O-glucoside...
Iridoids form a broad and versatile class of biologically active molecules found in thousands plant species. In addition to the many hundreds iridoids occurring plants, some iridoids, such as secologanin, serve key building blocks biosynthesis monoterpene indole alkaloids (MIAs) quinoline alkaloids. This study describes molecular cloning functional characterization three iridoid glucosyltransfeases (UDP-sugar glycosyltransferase6 [UGT6], UGT7, UGT8) from Madagascar periwinkle (Catharanthus...
C-Glucosyltransferase is an enzyme that mediates carbon-carbon bond formation to generate C-glucoside metabolites. Although it has been identified in several plant species, the catalytic amino acid residues required for C-glucosylation activity remain obscure. Here, we a 2-hydroxyflavanone C-glucosyltransferase (UGT708D1) soybean. We found three residues, His20, Asp85, and Arg292, of UGT708D1 were located at predicted active site evolutionarily conserved. The substitution Asp85 or Arg292...
Strictosidine synthetase, which catalyzes the condensation of tryptamine with secologanin to form strictosidine (isovincoside), was purified 740-fold homogeneity from cultured cells Catharanthus roseus in 10% yield. The specific activity is 5.85 nkat/mg. molecular weight as estimated by gel filtration 38,000. isoelectric point 4.6. Apparent Km values for and are 0.83 0.46 mM, respectively. enzyme shows a broad pH optimum between 5.0 7.5. product enzymic reaction exclusively strictosidine,...
Iridoids are one of the most widely distributed secondary metabolites in higher plants. They pharmacologically active principles various medicinal plants and key intermediates biosynthesis monoterpenoid indole alkaloids as well quinoline alkaloids. Although iridoids present 1-O-glucosides, glucosylation step biosynthetic pathway has remained obscure. We isolated a cDNA coding for UDP-glucose:iridoid glucosyltransferase (UGT85A24) from Gardenia jasminoides. UGT85A24 preferentially...
As nanoparticles can cross different cellular barriers and access tissues, control of their uptake fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show the trafficking single-walled carbon nanotubes (SWCNTs) through various subcellular membranes plant is facilitated or inhibited by attaching suitable tag controlling medium components. This enables unique over distribution SWCNTs provides key strategy promote elimination...
ABSTRACT The antimycobacterial activities of disulfiram (DSF) and diethyldithiocarbamate (DDC) against multidrug- extensively drug-resistant tuberculosis (MDR/XDR-TB) clinical isolates were evaluated in vitro . Both DSF DDC exhibited potent antitubercular 42 M. , including MDR/XDR-TB strains. Moreover, showed remarkable bactericidal activity ex vivo Therefore, might be a drug repurposed for the treatment MDR/XDR-TB.
Sugar–sugar glycosyltransferases play an important role in structural diversity of small molecule glycosides higher plants. We isolated a cDNA clone encoding sugar–sugar glucosyltransferase (CaUGT3) catalyzing 1,6-glucosylation flavonol and flavone glucosides for the first time from Catharanthus roseus. CaUGT3 exhibited unique glucosyl chain elongation activity forming not only gentiobioside but also gentiotrioside gentiotetroside sequential manner. investigated functional properties using...
Screening of a total 86 crude drugs for retinoid X receptor (RXR) ligands demonstrated that the methanol extract bark Magnolia obovata markedly activated transcriptional activity RXRα in luciferase reporter assays. Thereafter, honokiol (1) was isolated as constituent able to activate RXR selectively natural rexinoid, but not RARα. The 1 more potent than those phytanic acid and docosahexaenoic acid, both which are known be agonists. Honokiol is capable activating RXR/LXR heterodimer,...
A one‐pot system for efficient enzymatic synthesis of curcumin glucosides is described. The method couples the activities two recombinant enzymes, UDP‐glucose: glucosyltransferase from Catharanthus roseus (CaUGT2) and sucrose synthase Arabidopsis thaliana (AtSUS1). UDP, a product inhibitor UDP‐glucosyltransferase, was removed used regeneration UDP‐glucose by second enzyme, AtSUS1. productivity increased several‐fold initially added to reaction mixture could be reduced one‐tenth normal level....
Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring nutraceutical. For the first time, two glucosyltransferases UGT75L6 UGT94E5 that sequentially mediate final glucosylation steps crocin biosynthesis G. have been identified functionally characterized. preferentially glucosylates carboxyl group crocetin yielding glucosyl esters, while 6′ hydroxyl glucose moiety esters. The expression pattern neither nor correlated with accumulation .
The catalytic function of plant secondary product glycosyltransferases (PSPGs) was investigated by coupling the activities recombinant flavonoid glucosyltransferases having different regiospecificities with sucrose synthase from Arabidopsis thaliana . In present system, UDP, a inhibitor PSPGs, removed reaction mixture and used for regeneration UDP‐glucose AtSUS1. in situ system not only enhanced glucosylation efficiency but also unraveled novel regioselectivity PSPGs. effect shown to be...
Licorice (glycyrrhiza root) has been used as a herbal medicine worldwide with its main active constituent being glycyrrhizin (GL). sometimes causes adverse effects such inducing pseudoaldosteronism by inhibiting type 2 11β-hydroxysteroid dehydrogenase (11β-HSD2) caused glycyrrhetinic acid (GA), major metabolite of GL. In this study we compared the inhibitory GA, GL, and 3-monoglucuronyl-glycyrrhetinic (3MGA), another on 11β-HSD2 activity using microsomes rat kidney tissue slices. 3MGA, GL...
The p53 tumor suppressor plays critical roles in cell cycle regulation and apoptotic death response to various cellular stresses, thereby preventing cancer development. Therefore, the activation of through small molecules is an attractive therapeutic strategy for treatment cancers retaining wild-type p53. We used a library 700 Myanmar wild plant extracts identify that induce transcriptional activity. A cell-based screening method with p53-responsive luciferase-reporter assay system revealed...