A5101701597- PI3K/AKT/mTOR signaling in cancer
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Cancer-related molecular mechanisms research
- Plant Reproductive Biology
- Cancer Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Virus-based gene therapy research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Plant Gene Expression Analysis
- Nematode management and characterization studies
- Glioma Diagnosis and Treatment
- Legume Nitrogen Fixing Symbiosis
- Histone Deacetylase Inhibitors Research
- Head and Neck Cancer Studies
- Protein Degradation and Inhibitors
- Cancer, Lipids, and Metabolism
- Circular RNAs in diseases
- Nanoparticle-Based Drug Delivery
- Plant biochemistry and biosynthesis
Peking University Shenzhen Hospital
2018-2025
University of Hong Kong - Shenzhen Hospital
2025
Huazhong Agricultural University
2000-2025
Zhangjiagang First People's Hospital
2017-2025
Soochow University
2017-2025
Guangxi University
2025
Shanxi Medical University
2023-2024
Second Hospital of Shanxi Medical University
2023-2024
Robert W. Holley Center for Agriculture & Health
2020-2023
China Medical University
2014-2023
High-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, complete submergence. We showed previously that Arabidopsis (Arabidopsis thaliana) seedlings elevate the levels hundreds transcripts, including a core group 49 genes are prioritized for translation across cell types both shoots and roots. To recognize low-oxygen...
The transition metal copper (Cu) is essential for all living organisms but toxic when present in excess. To identify Cu deficiency responses comprehensively, we conducted genome-wide sequencing-based transcript profiling of Arabidopsis thaliana wild-type plants and a mutant defective the gene encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), which acts as transcriptional regulator responses. In response to deficiency, FERRIC REDUCTASE OXIDASE5 (FRO5) FRO4 levels increased strongly, an...
Simultaneous detection and imaging of multiple intracellular biomarkers hold great promise for early cancer detection. Here, we introduce a four-color nanoprobe that can simultaneously detect image four types mRNAs in living cells. The composed gold nanoparticles functionalized with dense shell molecular beacons, which identify mRNA transcripts. It shows rapid response, high specificity, nuclease stability, good biocompatibility. Intracellular experiments indicate the could effectively...
Traditional cancer therapies, such as surgery treatment, radiotherapy, and chemotherapy, often fail to completely eliminate tumor cells in an anaerobic microenvironment of regions. In contrast these traditional the use targeted delivery vectors deliver anticancer genes or antitumor drugs hypoxic areas tumors is most clinically promising treatment with rapid development recent years. this study, E.coli Nissle 1917 (EcN), intestinal probiotic, was utilized a transport vector p53 Tum-5 protein...
Abstract Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that Arabidopsis (Arabidopsis thaliana) Kelch domain-containing F-box proteins, AtKFB01, AtKFB20, and AtKFB50, function as negative regulators controlling via mediating PAL’s ubiquitination subsequent degradation. Here,...
Significance Cancer cells reprogram their metabolism in response to growth factor receptor mutations. However, the effect of altered nutrient levels on oncogenic signaling and therapeutic is not well understood. We demonstrate that glucose or acetate, two abundant “fuel” sources brain, promote epidermal vIII (EGFRvIII)-dependent through activation mechanistic target rapamycin complex 2 (mTORC2) by acetylation its core component Rictor. This activity mediated elevated acetyl-CoA. A surprising...
Suberin, a lipophilic polymer deposited in the outer integument of Arabidopsis (Arabidopsis thaliana) seed coat, represents an essential sealing component controlling water and solute movement protecting from pathogenic infection. Although many genes responsible for suberin synthesis are identified, regulatory components its biosynthesis have not been definitively determined. Here, we show that MYB107 transcription factor acts as positive regulator biosynthetic gene expression coat....
Purpose: Systemic administration of free chemotherapeutic drugs leads to severe toxic effects, and physiological characteristics solid tumors restrain the from reaching hypoxic regions.E. coli Nissle 1917 (EcN) has been known penetrate barrier proliferate in interface between viable necrotic regions tumors.This study aimed fabricate a nanoscale minicell via genetic engineering EcN for targeted delivery cancer therapy.Methods: A large number minicells were produced by knocking out minCD gene...
The highly lethal brain cancer glioblastoma (GBM) poses a daunting challenge because the blood-brain barrier renders potentially druggable amplified or mutated oncoproteins relatively inaccessible. Here, we identify sphingomyelin phosphodiesterase 1 (SMPD1), an enzyme that regulates conversion of to ceramide, as actionable drug target in GBM. We show brain-penetrant antidepressant fluoxetine potently inhibits SMPD1 activity, killing GBMs, through inhibition epidermal growth factor receptor...
The Arabidopsis BAP1 gene encodes a small protein with C2-like domain. Here we show that the is capable of binding to phospholipids in calcium-dependent manner and associated membranes vivo. We identify multiple roles negatively regulating defense responses cell death thaliana. loss function confers an enhanced disease resistance virulent bacterial oomycete pathogens. mediated by salicylic acid, PAD4 SNC1. also involved control death, which suggested altered hypersensitive response avirulent...
Abstract Here we identify the BAP1 and BAP2 genes of Arabidopsis (Arabidopsis thaliana) as general inhibitors programmed cell death (PCD) across kingdoms. These two homologous encode small proteins containing a calcium-dependent phospholipid-binding C2 domain. its functional partner BON1 have been shown to negatively regulate defense responses disease resistance gene SNC1. Genetic studies here reveal an overlapping function in control. The loss induces accelerated hypersensitive but does not...
Glioblastoma multiforme (GBM) is the most aggressive of astrocytic malignancies and common intracranial tumor in adults. Although epidermal growth factor receptor (EGFR) overexpressed and/or mutated at least 50% GBM cases required for maintenance animal models, EGFR inhibitors have thus far failed to deliver significant responses patients. One inherent resistance mechanism coactivation multiple tyrosine kinases, which generates redundancy activation phosphoinositide-3'-kinase (PI3K)...
Developing efficient methods for targeting cancer cells and encapsulating drugs coupled with activated release holds enormous potential cell imaging therapy. Herein, a novel dual-targeted nanocarrier was developed on the basis of gold nanoparticles modified dense shell synthetic oligonucleotides. The folic acid functionalized single-stranded DNA designed to target folate receptor surface, molecular beacon employed as drug carrier associated intracellular tumor mRNA. Intracellular experiments...
Abstract Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in plant cell wall. Manipulating monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs growth development. Here, we show that expressing an engineered 4- O -methyltransferase chemically modifies phenolic moiety monomeric precursors, thus preventing their incorporation into polymer, substantially alters hybrid...
Summary Copines are calcium‐dependent membrane‐binding proteins that highly conserved among protozoa, plants, nematodes and mammals. Although they implicated in membrane trafficking signal transduction, the functions of these not well understood. The Arabidopsis copine gene BON1/CPN1 was previously shown to negatively regulate a disease resistance ( R ) SNC1 . Here we report Arabidopsis, as other organisms, there is family genes, BON1 , 2 3 Using double triple mutant combinations show three...
SOX2 is a high-mobility group box containing transcription factor essential for the maintenance of embryonic stem cells. Recent evidence indicates that overexpression correlates with metastasis and poor prognosis in patients laryngeal squamous cell cancer. To investigate how contributes to this aggressive phenotype, we introduced human gene into low SOX2-expressing cancer line Hep-2. Cell migration invasion were determined by Transwell assay or without Matrigel coating. The...