A5102932979- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Nematode management and characterization studies
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Fungal and yeast genetics research
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Insect Pest Control Strategies
- Plant Disease Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Antimicrobial Peptides and Activities
- Invertebrate Immune Response Mechanisms
- Plant Parasitism and Resistance
- Plant and Fungal Interactions Research
- Plant Pathogens and Resistance
- Photosynthetic Processes and Mechanisms
- Plant pathogens and resistance mechanisms
- Insect and Arachnid Ecology and Behavior
- Biochemical and Structural Characterization
Jiangsu University
2024
Chinese Academy of Agricultural Sciences
2012-2023
Institute of Plant Protection
2014-2023
Fujian Academy of Agricultural Sciences
2022
University of California, Davis
2018
Chinese Academy of Agricultural Engineering
2014
Seoul National University
2012
Ministère de l'Agriculture et de la Souveraineté alimentaire
2010
Verticillium dahliae is a wide-host-range fungal pathogen that causes soil-borne diseases in hundreds of dicotyledonous hosts. In search V. Vd991 cell death-inducing proteins, we identified pectate lyase (VdPEL1) exhibited pectin hydrolytic activity, which could induce strong death several plants. Purified VdPEL1 triggered defense responses and conferred resistance to Botrytis cinerea tobacco cotton Our results demonstrated the mutant VdPEL1rec lacking enzymatic activity lacked functions...
Abstract Fusarium oxysporum is an important soilborne fungal pathogen with many different formae speciales that can colonize the plant vascular system and cause serious crop wilt disease worldwide. We found a glycoside hydrolase family 12 protein FoEG1, secreted by F. , acted as pathogen‐associated molecular pattern (PAMP) targeting apoplast of plants to induce cell death. Purified FoEG1 triggered death in induced defence response enhance resistance plants. The ability was mediated...
Background Magnaporthe oryzae, the rice blast fungus, might secrete certain proteins related to plant-fungal pathogen interactions. Methodology/Principal Findings In this study, we report purification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip1) secreted by M. oryzae. The fraction was purified identified de novo sequencing, sequence matched genomic putative from oryzae strain 70-15 (GenBank accession No. XP_366602.1)....
Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco.The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, the amino acid sequence identified mass spectrometry.This recombinant protein, expressed Escherichia coli an E1 expression vector, had good thermal stability, caused clearly defined hypersensitive response (HR) necrosis tobacco leaves.It could also trigger early defence events,...
BcGs1, a cell wall-degrading enzyme (CWDE), was originally derived from Botrytis cinerea. Our previous study revealed that BcGs1 could trigger defense responses and protect plants against various pathogens. We researched the response mechanism underlying this elicitation in tomato.We two domains were required for BcGs1's full necrosis activity. According to analysis quantitative real-time PCR of up-regulated proteins genes filtered by iTRAQ-based proteome approach, oxidative metabolism...
During pathogenic infection, hundreds of proteins that play vital roles in the Verticillium dahliae-host interaction are secreted. In this study, an integrated proteomic analysis secreted V. dahliae was performed, and a conserved secretory protein, designated VdCP1, identified as member SnodProt1 phytotoxin family. An expression vdcp1 gene revealed transcript is present every condition studied displays elevated throughout infection process. To investigate natural role VdCP1 dahliae, two...
Botrytis cinerea is one of the most notorious pathogenic species that causes serious plant diseases and substantial losses in agriculture throughout world. We identified BcXyl1 from B. exhibited xylanase activity. Expression gene was strongly induced infecting N. benthamiana tomato plants, deletion strains severely compromised virulence cinerea. strong cell death several activity independent its Purified triggered typically PTI responses conferred resistance to TMV tobacco plants. A 26-amino...
Alt a 1 family proteins (AA1s) have only been observed in the Dothideomycetes and Sordariomycetes classes of fungi, their biological functions remained poorly understood. Verticillium dahliae, soil-borne pathogen that causes plant wilt disease, secretes hundreds during process pathogenic infection, including AA1 member PevD1. In this study, we found pevd1 transcript was present all hosts studied (cotton, Arabidopsis, tomato, tobacco) showed elevated expression throughout infection process....
Abstract APETALA2/ethylene‐responsive factor (AP2/ERF) family transcription factors are well‐documented in plant responses to a wide range of biotic and abiotic stresses, but their roles mediating elicitor‐induced disease resistance remains largely unexplored. PevD1 is Verticillium dahliae secretory effector that can induce cotton tobacco plants. In our previous work, Nicotiana benthamiana ERF114 ( NbERF114 ) was identified screen genes differentially expressed response infiltration. Here,...
Here, we report the identification, purification, characterization and gene cloning of a novel hypersensitive response inducing protein secreted by necrotrophic fungus, Alternaria tenuissima, designated as 1 (Hrip1). The caused formation necrotic lesions that mimic typical apoptosis-related events including DNA laddering. protein-encoding was cloned rapid amplification cDNA ends (RACE) method. sequence analysis revealed is 495 bp in length open reading frame (ORF) encodes for polypeptide 163...
We report the identification, characterization, and gene cloning of a novel protein elicitor (PeBL1) secreted from Brevibacillus laterosporus strain A60. Through purification process consisting ion-exchange chromatography high-performance liquid (HPLC), we isolated that was identified by electrospray ionization quadrupole time flight tandem mass spectrometry (ESI-Q-TOF-MS-MS). The 351-bp PeBL1 produces 12,833-Da with 116 amino acids contains 30-residue signal peptide. expressed in...
C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles various physiological processes of life. In this study, zinc gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that encodes 24-kDa protein with 191 amino acids containing one typical domain; named NbZFP1. Transient expression pGDG-NbZFP1 demonstrated NbZFP1 localized to chloroplast, especially chloroplasts cells surrounding leaf stomata. Virus-induced silencing...
Panama disease, or Fusarium wilt, the most serious disease in banana cultivation, is caused by oxysporum f. sp. cubense (FOC) and has led to great economic losses worldwide. One effective way combat this enhancing host plant resistance. The cerato-platanin protein (CPP) family a group of small secreted cysteine-rich proteins filamentous fungi. CPPs as elicitors can trigger immune system resulting defense responses plants. In study, we characterized novel cerato-platanin-like secretome race 4...