This study was conducted to evaluate the effect of curcumin on laying performance, egg quality, biochemical indicators, hormone levels, and immune activity in hens under heat stress. Hy-Line brown (280-day-old) were fed with 0, 100, 150, 200 mg/kg during a 42-D experiment. Compared control treatment, supplementation 150 improved performance quality by significantly increasing production, eggshell thickness, strength (P < 0.01), albumen height 0.05) while decreasing feed-to-egg ratio....

Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco.The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, the amino acid sequence identified mass spectrometry.This recombinant protein, expressed Escherichia coli an E1 expression vector, had good thermal stability, caused clearly defined hypersensitive response (HR) necrosis tobacco leaves.It could also trigger early defence events,...

Abstract Background and objectives Quinoa ( Chenopodium quinoa Willd.) is a promising nutritious cereal known for its rich nutrition diverse physiological activities. In order to further investigate the profiles activities of phenolic compounds from quinoa, composition free bound extracts white, red, black were analyzed, as well their antioxidant, anti‐inflammatory, antitumor Findings Total content (TPC) total flavonoid (TFC) ranged 514.03 1,409.54 mg gallic acid equivalent (GAE)/100g 177.49...

Abstract BACKGROUND Quinoa protein is a potential source of bioactive peptides. Although some studies have demonstrated its angiotensin converting enzyme (ACE) inhibitory properties, research into in vivo effect on blood‐pressure regulation and peptide characterization remains limited. RESULTS hydrolyzate (QPH) was prepared by simulated gastrointestinal digestion. QPH lowered the systolic blood pressure (SBP) diastolic (DBP) spontaneously hypertensive model rats (SHRs) from 2 h to10 after...

This study systematically evaluated the effect of Forsythia suspensa extract on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) and determined its mechanism action. The results showed that significantly inhibited DSS-induced UC in mice. In vivo mechanistic studies revealed relieved symptoms by enhancing antioxidant activity inhibiting pyroptosis. Further vitro experiments it reduced level reactive oxygen species (ROS) J774A.1 cells. We found enhanced cellular antioxidation After...

This study aimed to investigate the effects of polyherbal mixtures (PHM) on growth performance, antioxidant capacities, immune function, and intestinal health in yellow-feathered broilers. PHM is composed five traditional Chinese medicine herbs (Portulaca oleracea L., Radix Sophora flavescens, Thalictrum glandulosissimum, Terra flava usta, Pogostemon cablin). A total 270 one-day-old broilers were randomly allotted into 3 treatments for a 42-d feeding trial, each with 6 replicates 15 birds....

Pathogens utilize a panoply of effectors to manipulate plant defense. However, despite their importance, relatively little is actually known about regulation these virulence factors. Here, we show that the effector Fol -Secreted Virulence-related Protein1 (FolSvp1), secreted from fungal pathogen Fusarium oxysporum f. sp. lycopersici ( ), directly binds and translocates tomato pathogenesis-related protein1, SlPR1, apoplast outside plasma membrane host nucleus via its nuclear localization...

Maize is one of the most important crops in world. However, great yield losses have been caused by several major diseases worldwide, including southern leaf blight (SLB), northern (NLB) and Fusarium stalk rot (FSR) (Mueller et al., 2020). Identification resistance genes cultivation genetic materials conferring multiple disease (MDR) effective ways to control reduce (Li limited MDR identified. Recently, it has proved that generation genome editing using CRISPR/Cas9 targeting susceptibility...

In ginseng, several ginsenosides have been demonstrated to alleviate dextran sulfate sodium (DSS)-induced colitis, especially the six in this study. However, which ginsenoside has most potent anti-inflammatory effect and may be selected as a promising candidate for treatment of colitis remains unclear. A cell inflammation model was induced by lipopolysaccharide (LPS) 12 h mouse sterile water containing DSS lasting seven days. Cytokines associated with inflammation, pyroptosis, ferroptosis...

Abstract Background Occludin, a tight junction protein, plays an important role in maintaining the integrity of lung epithelial barrier; however, its ventilation-induced injury has not been explored. Here, we measured expression occludin with different tidal volumes. Our study indicated that level was significantly decreased and alveolar permeability increased owing to acute injury. Methods Thirty healthy Wistar rats (15 female, 15 male) weighing 250–300 g, were randomly divided into 5...

Abstract Background Cinnamomum longepaniculatum (Gamble) N. Chao ex H. W. Li, whose leaves produce essential oils, is a traditional Chinese medicine and economically important tree species. In our study, two C . varieties that have significantly different oil contents leaf phenotypes were selected as the materials to investigate secondary metabolism. Result The content between varieties. When results of both transcriptome metabolomic analyses combined, it was found differences related...

Chinese herbs have been used as feed additives and are commonly utilized in domestic intensive livestock farming. However, their impact on the production performance intestinal health of broiler breeders has yet to be thoroughly explored. This study aimed evaluate effects a herbal mixture (CHM) terms reproductive hormones, antioxidant capacity, immunity, breeders. A total 336 thirty-wk-old hens were randomly allotted 4 groups with 6 replicates fourteen each, which fed basal diet supplemented...

Deoxynivalenol (DON) can affect health and growth performance of pigs, resulting in significant economic losses swine production. The aim this study was to investigate the effect glycyrrhizic acid combined with compound probiotics, i.e. Enterococcus faecalis plus Saccharomyces cerevisiae (GAP) on improving performance, intestinal its fecal microbiota composition change piglets challenged DON. A total 160 42-day-old weaned (Landrace × Large White) were used experimental period 28 d. results...

Botrytis cinerea, the causal agent of gray mold, is an important plant pathogen causing preharvest and postharvest diseases. Due to extensive use commercial fungicides, fungicide-resistant strains have emerged. Natural compounds with antifungal properties are widely present in various kinds organisms. Perillaldehyde (PA), derived from species Perilla frutescens, generally recognized as a potent antimicrobial substance be safe humans environment. In this study, we demonstrated that PA could...

Abstract Background Ventilator-induced lung injury (VILI) is characterized by increased alveolar permeability, pulmonary edema. The tyrosine kinase, c-Src, involved in VILI but its role has not been fully elucidated. This study examined the relationship between c-Src activation and occludin levels both vitro vivo . Methods For study, Wistar rats were randomly divided into five groups: control (group C); normal tidal volume M); + inhibitor (PP2) M P); high H); H P). Rats all groups group C...

The soil-borne fungal pathogen Verticillium dahliae infects a wide range of dicotyledonous plants including cotton, tobacco, and Arabidopsis. Among the effector proteins secreted by V. dahliae, 16 kDa PevD1 induces hypersensitive response in tobacco. Here we report high-resolution structure with folds resembling C2 domain-like calcium ion bound to C-terminal acidic pocket. A yeast two-hybrid screen, designed probe for molecular functions PevD1, identified Arabidopsis asparagine-rich protein...

SUMMARY Plants usually employ resistance ( R ) genes to defend against the infection of pathogens, and most encode intracellular nucleotide‐binding, leucine‐rich repeat (NLR) proteins. The recognition between proteins their cognate pathogens often triggers a rapid localized cell death at pathogen sites, termed hypersensitive response (HR). Metacaspases (MCs) belong cysteine protease family, structurally related metazoan caspases. MCs play crucial roles in plant immunity. However, underlying...

The enzyme myo-inositol oxygenase (MIOX) catalyzes the into glucuronic acid. In this study, 6 MIOX genes were identified from all of three diploid cotton species (Gossypium arboretum, Gossypium herbaceum and raimondii) Gossypioides kirkii, 12 two domesticated tetraploid cottons hirsutum, barbadense, 11 wild tomentosum, mustelinum darwinii. number in genome is roughly twice that genome. Members family classified six groups based on phylogenetic analysis. Integrated analysis collinearity...

Abstract Background Ventilator-induced lung injury (VILI) is one of the most common complications for patients with acute (ALI) or respiratory distress syndrome (ARDS). Although p120 an important protein in regulation cell junctions, further mechanisms should be explored prevention and treatment VILI. Methods Mouse epithelial cells (MLE-12), which were transfected small interfering (si)RNA, cDNA, wild-type E-cadherin juxtamembrane domain a K83R mutant (K83R-JMD), subjected to 20 % cyclic...