In order to increase nutritive values of soybean meal (SBM), 3 species microbes were used ferment SBM. Through a × orthogonal design and parameter measurements peptide anti-nutritional factor contents in the fermented (FSBM), it was estimated that best microbial proportion

Aflatoxin B1 (AFB1) and zearalenone (ZEA) are the secondary toxic metabolites of fungi which contaminate a wide range food feedstuffs. Limiting exposure humans livestock to them is very essential. Among numerous methods mycotoxin-degradation, biodegradation by microorganisms enzymes an effective promising approach eliminate their hazards. The present study aims optimize proportion different species beneficial microbes means response surface methodology (RSM) its combination with...

Deoxynivalenol (DON) is extensively detected in many kinds of foods and feeds to harm human animal health. This research aims investigate the effect chlorogenic acid (CGA) on alleviating inflammation apoptosis swine jejunal epithelial cells (IPEC-J2) triggered by DON. The results demonstrated that cell viability was decreased when DON concentrations increased or incubation time expanded. pretreatment with CGA (40 μg/mL) for 1 h viability, lactate dehydrogenase (LDH) release at 0.5 μg/mL 6 h,...

Zearalenone (ZEA) and aflatoxin B₁ (AFB₁) are two main kinds of mycotoxins widely existing in grain animal feed that cause a lot economic loss health problems for animals humans. In order to alleviate the cytotoxic effects AFB₁ ZEA on swine jejunal epithelial cells (IPEC-J2), combination cell-free supernatant compound probiotics (CFSCP) with mycotoxin degradation enzymes (MDEs) from Aspergillus oryzae was tested. The results demonstrated coexistence had synergetic toxic cell viability....

Deoxynivalenol (DON) is a common mycotoxin, which often induces oxidative stress and cytotoxicity in humans animals. Astilbin (AST), as natural antioxidant, exhibits multiple pharmacological functions. The aim of this study was to investigate the effects AST on alleviating DON-induced intestinal porcine epithelial cells (IPEC-J2). results demonstrated that 0.5 μg/mL DON stimulation for 6 hours induced stress, inflammation apoptosis IPEC-J2 cells. enhanced cell viability dose- time-dependent...

Abstract Aflatoxin B 1 (AFB ) is one of the most dangerous mycotoxins for humans and animals. This study aimed to investigate effects compound probiotics (CP), CP supernatant (CPS), AFB -degradation enzyme (ADE) on chicken embryo primary intestinal epithelium, liver kidney cell viabilities, determine functions + ADE (CPADE) or CPS (CPSADE) alleviating cytotoxicity induced by . The results showed that decreased viabilities in dose-dependent time-dependent manners. optimal concentrations...

The aim of this study was to evaluate the effects compound mycotoxin detoxifier (CMD) on alleviating toxic effect aflatoxin B1 (AFB1) for broiler growth performance. One-kilogram CMD consists 667 g B1-degrading enzyme (ADE, 1,467 U/g), 200 montmorillonite and 133 probiotics (CP). feeding experiment divided into 2 stages (1–21 d 22–42 d). In early stage, a total 300 one-day-old Ross broilers were randomly 6 groups, 5 replications each group, 10 (half male half female) in replication. later...

Deoxynivalenol (DON) can affect health and growth performance of pigs, resulting in significant economic losses swine production. The aim this study was to investigate the effect glycyrrhizic acid combined with compound probiotics, i.e. Enterococcus faecalis plus Saccharomyces cerevisiae (GAP) on improving performance, intestinal its fecal microbiota composition change piglets challenged DON. A total 160 42-day-old weaned (Landrace × Large White) were used experimental period 28 d. results...

This study investigated the effects of compound probiotics (CP) on AFB

Deoxynivalenol (DON) is one of the mycotoxins most frequently encountering in cereal-based foods throughout world. Saccharomyces cerevisiae was used to alleviate porcine jejunal epithelia cell (IPEC-J2) injury induced by DON this study. The results indicated that viability and proliferation rates were significantly decreased when concentrations increased from 0 64 µM after 24 h incubation (p < 0.05). longer time higher would cause more serious effects on viability. S. could degrade decrease...

In order to remove zearalenone (ZEA) detriment—Bacillus subtilis, Candida utilis, and cell-free extracts from Aspergillus oryzae were used degrade ZEA in this study. The orthogonal experiment vitro showed that the degradation rate was 92.27% (p &lt; 0.05) under conditions Bacillus subtilis SP1, SP2 mixed together at 0.5%, 1.0%, 1.0%. When combined with above probiotics a ratio of 2:1 make mycotoxin-biodegradation preparation (MBP), reached 95.15% 0.05). further investigate MBP effect on...

This study was conducted to investigate the effects of dietary supplementation with compound probiotics and berberine (CPB) on growth performance, nutrient digestibility fecal microflora in weaned piglets. A total 200 piglets 35 days old were randomly allocated 5 groups, 4 replications each group, 10 replication. Group basal diet; group B supplemented antibiotics zinc oxide; groups C, D E 0.06%, 0.12% 0.18% CPB, respectively. The experimental period 42 d. results indicated that there no...

This study investigated the effects of compound probiotics (CP) as starter cultures to reduce AFB1 residues and improve safety quality chicken jerky. First, Enterococcus faecalis, Candida utilis, Lactobacillus casein, Bacillus subtilis were subjected single strain fermentation experiments. Then, a three-factor, three-level response surface methodology design was used select CP based on degradation histamine content. The optimal included casein at initial viable counts 3.0 × 108, 109 CFU/mL...

Direct land application of conventional compost may cause ecological risks due to the presence heavy metals. To effectively reduce metal bioavailability in compost, a multi-component passivator comprising Candida utilis, sodium humate, zeolite and attapulgite was developed, which showed passivation rates 59.28%, 86.93% 38.95% for zinc (Zn), copper (Cu), ferrum (Fe), respectively, compost. The addition customized not only reduced mobility metals, but also improved quality further increased...

Deoxynivalenol (DON) is the most common mycotoxin that frequently contaminates human food and animal feed, resulting in intestinal diseases systemic immunosuppression. Glycyrrhinic acid (GA) exhibits various pharmacological activities. To investigate protective mechanism of GA for DON-induced inflammation apoptosis IPEC-J2 cells, RNA-seq analysis was used current study. The cells were treated with control group (CON), 0.5 μg/mL DON, 400 GA, GA+0.5 DON (GAD) 6 h. Results showed exposure h...

In order to alleviate the toxic effects of aflatoxins B1 (AFB1) on inflammatory responses in intestine, liver, and kidney broilers, aflatoxin B1-degrading enzyme, montmorillonite, compound probiotics were selected combined make a triple-action mycotoxin detoxifier (CMD). The feeding experiment was divided into two stages. early stage (1–21 day), total 200 one-day-old Ross broilers randomly four groups; later (22–42 160 aged at 22 days assigned groups: Group A: basal diet (4.31 μg/kg AFB1);...

Aflatoxins B1 (AFB1), deoxynivalenol (DON) and zearalenone (ZEA) are the three most prevalent mycotoxins, whose contamination of food feed is a severe worldwide problem. In order to alleviate toxic effects multi-mycotoxins (AFB1 + DON ZEA, ADZ) on inflammation apoptosis in swine jejunal epithelial cells (IPEC-J2), species probiotics (Bacillus subtilis, Saccharomyces cerevisiae Pseudomonas lactis at 1 × 105 CFU/mL, respectively) were mixed together make compound (CP), which further combined...

Five-hundred 1-day-old broilers were randomly assigned to five groups, 100 chickens for each group. From group 1 3, 80, 120 and 200 mg/kg soybean peptides was added the diets respectively; in fourth group, 3.2 genramycin added; fifth control without antibiotics. At age of 28 49 days, number goblet cells (GC), intestine intraepithelial lymphocyte, immunoglobulin A-forming cells, ratio villous height crypt depth (V/C) broiler's duodenum, jejunum cecum observed by application haematoxylin eosin...

Direct land application of conventional compost may cause ecological risks due to the presence heavy metals. To effectively reduce bioavailability metals in compost, a composite biological and physicochemical passivator was developed. It consisted 1.0 × 106 CFU/g Candida utilis 8% (9% sodium humate, 28% zeolite, 63% attapulgite, w/w), which showed 59.28%, 86.93% 38.95% passivation rates for zinc (Zn), copper (Cu) ferrum (Fe) respectively. The addition not only reduced mobility improved...

This study aimed to assess how the bacterial–enzyme co-fermentation of corn straw affects fermentation quality, digestion rate in Hu sheep, and rumen microorganisms. Orthogonal experiments were utilized establish optimal conditions, which subsequently applied trials involving four groups: group A (control), B (enzyme added), C (bacteria D + enzyme). The results show that conditions addition 2% flour, cottonseed meal, 60% moisture. In comparison with A, demonstrated most favorable outcomes,...

Deoxynivalenol (DON) is one of the most prevalent mycotoxin contaminants, which posing a serious health threat to animals and humans. Previous studies have found that individually supplemented probiotic or glycyrrhinic acid (GA) could degrade DON alleviate DON-induced cytotoxicity. The present study investigated effect combining GA with Saccharomyces cerevisiae (S. cerevisiae) Enterococcus faecalis (E. faecalis) using orthogonal design on alleviating IPEC-J2 cell damage induced by DON....

In order to estimate the effect of alpha-lipoic acid (LA) supplementation on relieving ammonia stress broilers, 180 22-day-old male broilers were assigned three groups, six replicates in each group and 10 birds per replicate. The groups were: (1) a control without stress; (2) exposure 70 ppm atmospheric (AM); (3) administration 300 mg/kg LA (AM + LA). experimental period was 3 weeks. Results showed that average daily weight gain increased feed conversion ratio decreased AM group, compared...

Deoxynivalenol (DON) is a widespread mycotoxin that affects the intestinal health of animals and humans. In present study, we performed RNA-sequencing 16S rRNA sequencing in piglets after DON glycyrrhizic acid compound probiotics (GAP) supplementation to determine changes transcriptome microbiota. Transcriptome results indicated exposure altered gene expression involved nutrient transport metabolism. Genes related lipid metabolism, such as PLIN1, PLIN4, ADIPOQ, FABP4 intestine, were...