A5102833552- Intensive Care Unit Cognitive Disorders
- Machine Learning in Bioinformatics
- S100 Proteins and Annexins
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Inflammasome and immune disorders
- Anesthesia and Neurotoxicity Research
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Heme Oxygenase-1 and Carbon Monoxide
- Genomics and Phylogenetic Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- DNA and Nucleic Acid Chemistry
- Anesthesia and Sedative Agents
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Acupuncture Treatment Research Studies
- Cardiac and Coronary Surgery Techniques
Shandong University
2011-2024
Shandong Provincial Hospital
2024
Shandong First Medical University
2019-2024
Shandong Provincial QianFoShan Hospital
2011-2022
Affiliated Hospital of Taishan Medical University
2022
Guangxi Zhuang Autonomous Region Hydraulic Research Institute
2008
Academy of Military Medical Sciences
2008
Nanjing University
2005
Objective: Rosiglitazone, a potent agonist of peroxisome proliferator-activated receptor (PPAR)-γ, exerts anti-inflammatory effects in vitro and vivo. This study was designated to determine the rosiglitazone on endotoxin-induced acute lung injury rats. Design: Prospective, experimental study. Setting: University research laboratory. Subjects: Thirty-six male Wistar Interventions: All animals were randomly assigned one six groups (n = 6 per group) given either lipopolysaccharide (6 mg/kg...
Lung hyperinflation is known to be an important contributing factor in the pathogenesis of ventilator-induced lung injury. Mechanical stretch causes epithelial barrier dysfunction and increase alveolar permeability, although precise mechanisms have not been completely elucidated. p120-catenin adherens junction-associated protein that regulates cell-cell adhesion. In this study, we determined role cyclic stretch-induced dysfunction. Cultured cells (MLE-12) were subjected uniform (0.5 Hz)...
Abstract Background At present, sevoflurane inhalation anesthesia used on infants is well-known. But long-time exposure to anesthetic could cause neurologic disorder, especially nerve degeneration in infant and developing brain. The central nervous system of affect the memory cognitive function. γ-Aminobutyric acid (GABA) a known inhibitory neurotransmitter system. Inhalation may activate GABA A receptor inhibit system, leading apoptosis neural degeneration, dysfunction critical period brain...
Postoperative cognitive dysfunction (POCD) is a common postoperative complication observed in elderly patients. However, the diagnosis of POCD not very satisfactory as no specific biomarkers have been classified. It necessary to identify new diagnostic markers better understand pathogenesis POCD. We performed liquid chromatography with time-of-flight mass spectrometer- (LC/Q-TOF-MS-) based metabolomics study investigate A total 40 metabolites were differentially expressed between and...
To find out risk factors for postoperative cognitive dysfunction (POCD) after coronary artery bypass grafting (CABG), and to provide basis clinical prevention of POCD. A total 88 patients who underwent CABG were surveyed with Telephone Questionnaire (TICS-M) their impairment 3, 7, 21, 90, 180 days post-surgery. The occurrence POCD was diagnosed by Neuropsychological Battery which included Vocabular Learning Test (VLT), Wisconsin Card Sorting (WCST), Trail Making (TMT) Symbol Digit Modalities...
Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value A. palmatum. However, molecular mechanisms responsible for changes remain unclear.We chose palmatum cultivars yellow ('Jinling Huangfeng') red Danfeng') leaves ideal material studying complex metabolic networks variations in coloration. The 24 libraries obtained from four different time points growth 'Jinling...
Some microorganisms can produce pigments such as melanin, which has been associated with virulence in the host and a survival advantage environment. In Vibrio cholerae, studies have shown that pigment-producing mutants are more virulent than parental strain terms of increased UV resistance, production major factors, colonization. To date, almost all pigmented V. cholerae strains investigated induced by chemicals, culture stress, or transposon mutagenesis. However, during our cholera...
The molecular mechanisms underlying sevoflurane (SEVO)-induced impairment of learning and memory remain unclear. Specifically, a role microRNAs (miRNAs) in the control neuron proliferation developing brain exposed to SEVO has not been reported previously. Here, we studied effects exposure on neural cell proliferation, neonatal rats. We found that significantly decreased reduced BDNF levels brain, impaired rats Morris water maze test Plus-Maze discriminative avoidance task (PM-DAT), likely...
Previous experiments revealed phospholipid scramblase 4 (PLSCR4) mRNA to be significantly increased in a lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) model of human pulmonary microvascular endothelial cells (HPMECs); however, the effect PLSCR4 and its mechanism have not been reported date. The PLSCR family is thought mediate transmembrane movement phospholipids (PS), has found involved pyroptosis through combing with gasdermin D (GSDMD). We therefore speculated...
Establishing codon usage biases are crucial for understanding the etiology of central nervous system neurodegenerative diseases (CNSNDD) especially Alzheimers disease (AD) as well genetic factors. G and C ending codons strongly biased in coding sequences these proteins a result genomic GC composition constraints. On other hand, that identified translationally optimal major trend all end or G, suggesting translational selection should also be taken into consideration additional to...
Ventilator-induced lung injury (VILI) is commonly associated with respiratory barrier dysfunction; however, the mechanisms have not been fully elucidated. This study aimed to determine order and components of signalling pathway that mediates degradation adherin junction p120-catenin in VILI.For vivo study, C57BL/6 mice were pre-treated inhibitors for 60 min prior 4 h mechanical ventilation. For vitro mouse epithelial 12 (MLE-12) cells or small interfering RNA (siRNA) 48 cyclic stretch at 20%...
Inflammation may play a role in postoperative cognitive dysfunction. 5′ Adenosine monophosphate-activated protein kinase, nuclear factor-kappa B, interleukin-1β, and tumor necrosis factor-α are involved inflammation. Therefore, these inflammatory mediators be Western immunoblot analysis revealed adenosine kinase B the hippocampus of aged rats were increased 1–7 days after splenectomy. Moreover, interleukin-1β upregulated gradually decreased. participate splenectomy model dysfunction rats.
Abstract Background With the development of experimental techniques and bioinformatics, quantity data available from protein-protein interactions (PPIs) is increasing exponentially. Functional modules can be identified protein interaction networks. It follows that investigation functional will generate a better understanding cellular organization, processes, functions. However, PPI are still limited, no modularity analysis PPIs in pathogens has been published to date. Results In this study,...
Recombinant human annexin A5 (Anx5) is known to protect cardiac function during endotoxemia, although the underlying mechanisms have yet be elucidated. In this study, we demonstrated that Anx5 could repair disrupted cardiomyocyte adherens junctions and improve myocardial contractile in lipopolysaccharide (LPS)-induced endotoxemia. Mechanistic studies revealed antagonize disassociation between p120-catenin (p120) N-cadherin as well dephosphorylation of p120 LPS-treated cardiomyocytes. Small...
Despite over 50 years of clinical and basic studies, acute respiratory distress syndrome (ARDS) is still a critical challenge with high mortality worldwide. The severity neutrophil activation was associated disease severity. However, the detailed pathophysiology circulating polymorphonuclear in ARDS remains unclear. To identify key pathways genes ARDS-specific phenotype distinct from sepsis, datasets blood neutrophils (PMNs) patients (GSE76293) sepsis (GSE49757) were chosen Gene Expression...
Computational identification of transcription factor binding sites (TFBSs) is a rapid, cost-efficient way to locate unknown regulatory elements. With increased potential for high-throughput genome sequencing, the availability accurate computational methods TFBS prediction has never been as important it currently is. To date, identifying TFBSs with high sensitivity and specificity still an open challenge, necessitating development novel models predicting factor-binding DNA elements.Based on...
The SNP -158G>A of KLK3 has been validated as a regulatory (rSNP) by molecular biology assays, but the mechanism how it affects binding an androgen receptor (AR) homodimer with DNA is unclear. In current study, dynamics simulation was adopted to explain its inner cause. Based on recent review), three types intermolecular forces were analyzed, and differences among them compared between complexes containing -158 A:T G:C. Extra hydrophobic contacts caused methyl group mutated thymine most...