A5065827653- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- GABA and Rice Research
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Cancer Research and Treatments
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Biochemical and Structural Characterization
- Microbial bioremediation and biosurfactants
- Chromatography in Natural Products
- Civil and Geotechnical Engineering Research
- Calcium signaling and nucleotide metabolism
Hubei University
2016-2025
Huazhong Agricultural University
2014
Abstract Background Biofuels and value-added biochemicals derived from renewable biomass via biochemical conversion have attracted considerable attention to meet global sustainable energy environmental goals. Isobutanol is a four-carbon alcohol with many advantages that make it attractive as fossil-fuel alternative. Zymomonas mobilis highly efficient, anaerobic, ethanologenic bacterium making promising industrial platform for use in biorefinery. Results In this study, the effect of...
The cyclodipeptide pulcherriminic acid synthesized by Bacillus licheniformis is an iron chelator that antagonizes certain pathogens removing from the environment. But since insoluble iron-pulcherriminic complex cannot act as carrier siderophores do, excessive causes starvation for producer cells. At present, regulation of synthesis and mechanism which B. strikes a balance between biocontrol self-protection removal remain unclear. This study provides insights into regulatory network explains...
Iturin A is a biosurfactant with various applications, and its low synthesis capability limits production application development. Fatty acids play critical role in cellular metabolism target product syntheses, the relationship between fatty acid supplies iturin unclear. In this study, we attempted to increase via strengthening pathways Bacillus amyloliquefaciens. First, acetyl-CoA carboxylase AccAD ACP S-malonyltransferase fabD were overexpressed promoter replacement, yield was increased...
The salt–tolerant yeast Zygosaccharomyces rouxii is a typical aroma–producing used in food brewing, but its mechanism of high temperature tolerance still unclear. In this study, the response Z. to glucose under stress at 40 °C was explored, based on total synthetic lowest–nutrient medium. results growth curves and scanning electron microscopy showed that necessary for restore stress, with biomass 300 g/L (OD600, 120h = 2.44 ± 0.26) being 8.71 times higher than 20 0.28 0.08). transcriptome...
ABSTRACT Streptomyces fradiae is an important bioresource to produce various antibacterial natural products, however, the time-consuming and labor-intensive genome editing toolkits hindered construction application of engineered strains, this study aimed establish efficient CRISPR/Cas9n system in S. . Initially, CRISPR/Cas9-mediated tool was employed replace those awkward tools that relied on homologous recombination, while off-target Cas9 exhibited high toxicity Sf01. Therefore, nickase...
Tobacco specific nitrosamines (TSNA) mainly consisting of N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are a group toxic components threatening human health. To inhibit TSNA formation in tobacco leaves, high nitrite reductive strain with low nitrate reduction ability was isolated applied to leaves an attempt lower the precursor TSNA. By morphology, physiology, biochemistry, 16S rDNA sequence analysis,...
Bacitracin, a type of cyclic dodecapeptide antibiotic mainly produced by Bacillus, is widely used in fields veterinary drug and feed additive. Modularization metabolic pathways based on the concept synthetic biology has been efficient synthesis target products. Here, we want to improve bacitracin production through strengthening aspartic acid (Asp) supply B. licheniformis DW2. First, exogenous Asp addition assays implied that benefited production. Second, were strengthened via overexpressing...
Bacillus licheniformis DW2 is an important industrial strain for bacitracin production, and it also used biochemical however, the lack of effective toolkit precise regulation gene expression hindered its application seriously. Here, a gradient strength promoter library was constructed based on synthetase cluster PbacA. First, different PbacA variants were via coupling with various 5′-UTRs, ranges 32.6–741.8% attained among these promoters. Then, three promoters, PUbay (strong), (middle),...
Nicotinamide riboside (NR) is an important nucleotide, a precursor of nicotinamide adenine dinucleotide, that has attracted great attention as promising nutraceutical to improve various symptoms diabetes, vascular disease, and age-related physiological decline. Here, we systematically engineered Bacillus licheniformis produce NR from glucose (NAM) efficiently. Specifically, selected the pathway using phosphoribosyltransferase (NAMPT) for converting NAM mononucleotide (NMN) in presence...
Owing to the feature of strong α-glucosidase inhibitory activity, 1-deoxynojirimycin (1-DNJ) has broad application prospects in areas functional food, biomedicine, etc., and this research wants construct an efficient strain for 1-DNJ production, basing on Bacillus amyloliquefaciens HZ-12. Firstly, using temperature-sensitive shuttle plasmid T2 (2)-Ori, gene ptsG phosphotransferase system (PTS) was weakened by homologous recombination, non-PTS pathway strengthened deleting its repressor iolR,...
Bacitracin, a new type of cyclic peptide antibiotic, is widely used as the feed additive in industry. Branched chain amino acids (BCAAs) are key precursors for bacitracin synthesis. In this research, soybean meal was served raw material to supply precursor synthesis, and enhanced production attempted by engineering BCAA transporter BrnQ its regulator Lrp industrial strain Bacillus licheniformis DW2. Firstly, our results confirmed that negatively affected synthesis DW2, deletion lrp improved...
Establishment of a green and sustainable process for production the antioxidant hydroxytyrosol using metabolically engineered Bacillus licheniformis .
The cyclodipeptide pulcherriminic acid, produced by Bacillus licheniformis, is derived from cyclo(l-Leu-l-Leu) and possesses excellent antibacterial activities. In this study, we achieved the high-level production of acid via multistep metabolic engineering B. licheniformis DWc9n*. First, increased leucine (Leu) supply overexpressing ilvBHC-leuABCD operon ilvD, involved in Leu biosynthesis, to obtain strain W1, engineered W2 was further attained deletion gene bkdAB, encoding a branched-chain...
Bacitracin is a broad-spectrum cyclic peptide antibiotic mainly produced by Bacillus, precursor amino acid supply served as the critical role during its synthesis. In this study, we systematically engineered branch-chain (BCAA) modules for bacitracin production. Firstly, demonstrated that Ile and Leu acted limiting precursors synthesis, BCAA synthetic pathways were strengthened via simultaneous overexpression of, feedback-resistance acetolactate synthase IlvBNfbr, 2-isopropylmalate...