A5102892563- Concrete and Cement Materials Research
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Innovations in Concrete and Construction Materials
- Innovative concrete reinforcement materials
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Biopolymer Synthesis and Applications
- Enzyme Structure and Function
- Magnesium Oxide Properties and Applications
- Grouting, Rheology, and Soil Mechanics
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Microbial Metabolism and Applications
- GABA and Rice Research
- Enzyme Production and Characterization
- Biological and pharmacological studies of plants
- Mesoporous Materials and Catalysis
- Process Optimization and Integration
- Smart Materials for Construction
- Plant and Biological Electrophysiology Studies
- Endoplasmic Reticulum Stress and Disease
- Chromatography in Natural Products
- thermodynamics and calorimetric analyses
Hubei University
2017-2025
ZheJiang Academy of Agricultural Sciences
2024
Zhejiang University
2024
Nanjing Normal University
2022-2024
University of Jinan
2022-2024
Wuhan University
2006
Pig gastrointestinal tracts harbor a heterogeneous and dynamic ecosystem populated with trillions of microbes, enhancing the ability host to harvest energy from dietary carbohydrates contributing adipogenesis fatness. However, microbial community structure related mechanisms responsible for differences between fatty phenotypes lean pigs remained be comprehensively elucidated. Herein, we first found significant in composition potential functional capacity among different gut locations Jinhua...
Abstract Poly-γ-glutamic acid (γ-PGA) is an important biochemical product with a variety of applications. This work reports novel approach to improve γ-PGA through over expression key enzymes in cofactor NADPH generating process for pool. Six genes encoding the generation were over-expressed producing strain B. licheniformis WX-02. Among various recombinants, over-expressing zwf gene (coding glucose-6-phosphate dehydrogenase), WX-zwf, produced highest concentration (9.13 g/L), 35%...
Primary metabolism plays a key role in the synthesis of secondary metabolite. In this study, main transcription factors carbon, nitrogen, and phosphorus metabolisms (CcpA, CcpC, CcpN, CodY, TnrA, GlnR, PhoP) were engineered to improve bacitracin yield Bacillus licheniformis DW2, an industrial strain for production. First, our results demonstrated that deletions ccpC ccpN improved ATP NADPH supplies, yields respectively increased by 14.02% 16.06% compared with while it was decreased...
ABSTRACT Streptomyces fradiae is an important bioresource to produce various antibacterial natural products, however, the time-consuming and labor-intensive genome editing toolkits hindered construction application of engineered strains, this study aimed establish efficient CRISPR/Cas9n system in S. . Initially, CRISPR/Cas9-mediated tool was employed replace those awkward tools that relied on homologous recombination, while off-target Cas9 exhibited high toxicity Sf01. Therefore, nickase...
Bacitracin, a type of cyclic dodecapeptide antibiotic mainly produced by Bacillus, is widely used in fields veterinary drug and feed additive. Modularization metabolic pathways based on the concept synthetic biology has been efficient synthesis target products. Here, we want to improve bacitracin production through strengthening aspartic acid (Asp) supply B. licheniformis DW2. First, exogenous Asp addition assays implied that benefited production. Second, were strengthened via overexpressing...
Bacitracin, a new type of cyclic peptide antibiotic, is widely used as the feed additive in industry. Branched chain amino acids (BCAAs) are key precursors for bacitracin synthesis. In this research, soybean meal was served raw material to supply precursor synthesis, and enhanced production attempted by engineering BCAA transporter BrnQ its regulator Lrp industrial strain Bacillus licheniformis DW2. Firstly, our results confirmed that negatively affected synthesis DW2, deletion lrp improved...
Bacitracin is a broad-spectrum veterinary antibiotic that widely used in the fields of drug and feed additive. S-Adenosylmethionine (SAM) critical factor involved many biochemical reactions, especially production. However, whether SAM affects bacitracin synthesis still unknown. Here, we want to analyze relationship between supply synthesis, then metabolic engineering synthetic pathway for production Bacillus licheniformis. Firstly, our results implied exogenous addition benefited production,...
The cyclodipeptide pulcherriminic acid, produced by Bacillus licheniformis, is derived from cyclo(l-Leu-l-Leu) and possesses excellent antibacterial activities. In this study, we achieved the high-level production of acid via multistep metabolic engineering B. licheniformis DWc9n*. First, increased leucine (Leu) supply overexpressing ilvBHC-leuABCD operon ilvD, involved in Leu biosynthesis, to obtain strain W1, engineered W2 was further attained deletion gene bkdAB, encoding a branched-chain...
Bacitracin is a broad-spectrum cyclic peptide antibiotic mainly produced by Bacillus, precursor amino acid supply served as the critical role during its synthesis. In this study, we systematically engineered branch-chain (BCAA) modules for bacitracin production. Firstly, demonstrated that Ile and Leu acted limiting precursors synthesis, BCAA synthetic pathways were strengthened via simultaneous overexpression of, feedback-resistance acetolactate synthase IlvBNfbr, 2-isopropylmalate...
Due to low early strength and high shrinkage, ordinary Portland cement (OPC) is difficult meet the actual needs of construction projects, while Belite calcium sulfoaluminate (BCSA-OPC) composite provides a new solution. The mechanical drying shrinkage properties BCSA-OPC mortar were determined, hydration heat was studied, pore size distribution investigated by mercury injection pressurization (MIP). Besides, products analyzed X-ray diffraction (XRD) simultaneous thermal analysis (TG-DSC),...