A5010323145- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Lipid metabolism and biosynthesis
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Gene Regulatory Network Analysis
- Enzyme Structure and Function
- Invertebrate Immune Response Mechanisms
- Microbial Metabolites in Food Biotechnology
- Soybean genetics and cultivation
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Plant Taxonomy and Phylogenetics
- Process Optimization and Integration
- Aquatic life and conservation
- Advanced biosensing and bioanalysis techniques
Nanjing Normal University
2019-2025
Forest Trends
2023
Virginia Tech
2023
Shandong First Medical University
2022
Shandong Tumor Hospital
2022
Nanjing Tech University
2016-2019
Fondation Maison des Sciences de l'Homme
2019
The Synergetic Innovation Center for Advanced Materials
2016
Schizochytrium sp. is a marine microalga with great potential as promising sustainable source of lipids rich in docosahexaenoic acid (DHA). This organism's lipid accumulation machinery can be induced by various stress conditions, but this induction usually comes at the expense lower biomass industrial fermentations. Moreover, oxidative damage environmental stresses result peroxidation lipids, and especially polyunsaturated fatty acids, which causes unstable DHA production, often ignored...
Escherichia coli is the most widely used bacterium in prokaryotic expression system for production of recombinant proteins. In BL21 (DE3), gene encoding T7 RNA polymerase (T7 RNAP) under control strong lacUV5 promoter (PlacUV5), which leakier and more active than wild-type lac (PlacWT) certain growth conditions. These characteristics are not advantageous those proteins with toxic or growth-burdened. On one hand, leakage RNAP leads to rapid target non-inducing period, sucks resources away...
Controlling the copy number of gene expression cassettes is an important strategy to engineer bacterial cells into high-efficiency biocatalysts. Current strategies mostly use plasmid vectors, but multicopy plasmids are often genetically unstable, and their numbers cannot be precisely controlled. The integration a chromosome has advantages, iterative laborious, it challenging obtain library with varied doses for phenotype characterization. Here, we demonstrated that chromosomal using...
Schizochytrium sp. is a promising strain for the production of docosahexaenoic acid (DHA)-rich oil and biodiesel, has been widely used in food additive bioenergy industries. Oxygen particularly important environmental factor cell growth DHA synthesis. In general, higher oxygen supply favors lipid accumulation, but could lead to reduction percentage total fatty acids To tackle this problem, it essential understand mechanisms regulating response oxygen. study, we aimed explore acclimatization...
The sesquiterpene α-humulene has been shown to have anti-inflammatory and anticancer activities, which led its vast application potential in medicine. However, production methods including phytoextraction chemical synthesis currently were limited low yield, high costs, expensive catalysts, cannot meet the increasing market demand. In this study, Yarrowia lipolytica was developed as a robust cell factory for production. peroxisome Y. first engineered boost of α-humulene. By...
ABSTRACT Gastrodin, the principal bioactive component of renowned herb Gastrodia elata , is extensively utilized in medicinal drugs and nutraceuticals. This study seeks to enhance microbial production gastrodin through high‐throughput screening (HTS) transcriptomics‐guided optimization. Initially, atmospheric pressure room temperature plasma (ARTP)‐mediated mutagenesis were employed develop a library mutant strains. Furthermore, transcription factor‐based biosensor solid‐phase extraction...
Presently, the supply of eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) traditionally produced by marine fisheries will be insufficient to meet their market demand in food industry. Thus a sustainable alternative source is urgently required. Schizochytrium sp. an ideal producer DHA; however, its ability co-produce DHA EPA has not yet been proved. Herein, we first described cobalamin-independent methionine synthase-like (MetE-like) complex, which contains independent acyltransferase...
Non-homologous end-joining (NHEJ)-mediated random integration in Yarrowia lipolytica has been demonstrated to be an effective strategy for screening hyperproducer strains. However, there was no multigene assembly method applied NHEJ integration, which made it challenging construct and integrate metabolic pathways. In this study, a Golden Gate modular cloning system (YALIcloneNHEJ) established develop robust DNA platform Y. lipolytica. By optimizing key factors, including the amounts of...
Abstract Background Eicosapentaenoic acid (EPA) is widely used in the functional food and nutraceutical industries due to its important benefits human health. Oleaginous microorganisms are considered a promising alternative resource for production of EPA lipids. However, storage triglyceride (TG) becomes key factor limiting level. Results This study aimed incorporate more into TG through metabolic engineering. Firstly, enzymes synthesis, diacylglycerol acyltransferase ( DGAT )...